Construct: ORF TRCN0000476713
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014699.1_s317c1
- Derived from:
- ccsbBroadEn_00433
- DNA Barcode:
- CTACCTCTGGCCATGAGCAATTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DECR1 (1666)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476713
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1666 | DECR1 | 2,4-dienoyl-CoA reductase 1 | NM_001359.2 | 100% | 100% | |
| 2 | human | 1666 | DECR1 | 2,4-dienoyl-CoA reductase 1 | NM_001330575.1 | 94.9% | 92% | (many diffs) |
| 3 | human | 1666 | DECR1 | 2,4-dienoyl-CoA reductase 1 | XM_017013148.1 | 94.9% | 92% | (many diffs) |
| 4 | human | 1666 | DECR1 | 2,4-dienoyl-CoA reductase 1 | XM_011516880.3 | 94.3% | 93.7% | (many diffs) |
| 5 | human | 1666 | DECR1 | 2,4-dienoyl-CoA reductase 1 | XM_005250808.5 | 72.5% | 72.5% | 0_1ins276 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1074
- ORF length:
- 1005
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaagctaccg gccagggttt tctttactct ggggtcccgg ctgccctgtg 121 gcctcgctcc tcggaggttt ttcagttatg ggacaaaaat attatatcaa aacactgaag 181 ctttgcaatc taaattcttt tcacctcttc aaaaagcgat gctaccacct aatagttttc 241 aaggaaaagt ggcattcatt actgggggag gtactggcct tggtaaagga atgacaactc 301 ttctgtccag cctaggtgct cagtgcgtga tagccagccg gaagatggat gttttgaaag 361 ctaccgcaga acaaatttct tctcaaactg gaaataaggt tcatgcaatt cagtgtgatg 421 tgagggatcc tgatatggtt caaaacactg tgtcagaact gatcaaagtt gcaggacatc 481 ctaatattgt gataaacaat gcagcaggga attttatttc tcctactgaa agactttctc 541 ctaatgcttg gaaaaccata actgacatag ttctaaatgg cacagccttc gtgacactag 601 aaattggaaa acaactaatt aaagcacaga aaggagcagc atttctttct attactacta 661 tctatgctga gactggttca ggttttGTAG TACCAAGTGC TTCTGCCAAA GCAGGTGTGG 721 AAGCCATGAG CAAGTCTCTT GCAGCTGAAT GGGGTAAATA TGGAATGCGA TTCAATGTGA 781 TTCAACCAGG GCCTATAAAA ACCAAAGGTG CCTTTAGCCG TCTGGACCCA ACTGGAACAT 841 TTGAGAAAGA AATGATTGGC AGAATTCCCT GTGGTCGCCT GGGGACTGTA GAAGAACTCG 901 CAAATCTTGC TGCTTTCCTT TGTAGTGATT ATGCTTCTTG GATTAATGGA GCAGTCATTA 961 AATTTGACGG TGGAGAGGAA GTACTTATTT CAGGGGAATT CAACGACCTG AGAAAGGTCA 1021 CCAAGGAGCA GTGGGACACC ATAGAAGAAC TCATCAGGAA GACAAAAGGT TCCTTGCCAA 1081 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1141 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1201 TATCTTGTGG AAAGGACGAC TACCTCTGGC CATGAGCAAT TTTACGCGTT AAGTCgacaa 1261 tcaacctctg gattacaaaa tttgtgaaag att