Construct: ORF TRCN0000476718
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006835.1_s317c1
- Derived from:
- ccsbBroadEn_08864
- DNA Barcode:
- TAATATAACGCCTCGTATCTGATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MTARC1 (64757)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476718
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64757 | MTARC1 | mitochondrial amidoxime red... | NM_022746.4 | 99.8% | 99.4% | 493A>G;560T>A |
| 2 | human | 64757 | MTARC1 | mitochondrial amidoxime red... | XM_011509900.3 | 95% | 94.6% | 493A>G;560T>A;752_802del |
| 3 | human | 64757 | MTARC1 | mitochondrial amidoxime red... | XM_017002096.2 | 72.2% | 72.4% | (many diffs) |
| 4 | human | 64757 | MTARC1 | mitochondrial amidoxime red... | XM_017002097.2 | 69.5% | 69.1% | 0_1ins306;187A>G;254T>A |
| 5 | human | 64757 | MTARC1 | mitochondrial amidoxime red... | XM_011509903.3 | 68.8% | 68.9% | (many diffs) |
| 6 | human | 64757 | MTARC1 | mitochondrial amidoxime red... | XR_001737362.1 | 67.3% | (many diffs) | |
| 7 | human | 64757 | MTARC1 | mitochondrial amidoxime red... | XM_011509904.3 | 66.1% | 65.8% | (many diffs) |
| 8 | human | 64757 | MTARC1 | mitochondrial amidoxime red... | XR_921908.1 | 58.2% | (many diffs) | |
| 9 | human | 64757 | MTARC1 | mitochondrial amidoxime red... | XR_002957377.1 | 35.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1077
- ORF length:
- 1011
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg cgccgccggc tcctccgcgc tggcgcgctt tgtcctcctc gcgcaatccc 121 ggcccgggtg gctcggggtt gccgcgctgg gcctgaccgc ggtggcgctg ggggctgtcg 181 cctggcgccg cgcatggccc acgcggcgcc ggcggctgct gcagcaggtg ggcacagtgg 241 cgcagctctg gatctaccct gtgaaatcct gcaagggggt gccggtgagc gaggcggagt 301 gcacggccat ggggctgcgc agcggcaacc tgcgggacag gttttggctt gtgatcaacc 361 aggagggaaa catggttact gctcgccagg aacctcgcct ggtcctgatt tccctgacct 421 gcgatggtga caccctgact ctcagtgcag cctacacaaa ggacctacta ctgcctatca 481 aaacgcccac cacaaatgca gtgcacaagt gcagagtgca cggcctggag atagagggca 541 gggactgtgg cgaggccgcc gcccagtgga taaccagctt cctgaagtca cagccctacc 601 gcctggtgca cttcgagcct cacaagcgac cgagacgtcc tcatcaaata gcagacttgt 661 tccgacccaa ggaccagatt gcttactcag acaccagccc attcttgatc ctttcTGAGG 721 CGTCGCTGGC GGATCTCAAC TCCAGGCTAG AGAAGAAAGT TAAAGCAACC AACTTCAGGC 781 CCAATATTGT AATTTCAGGA TGCGATGTCT ATGCAGAGGA TTCTTGGGAT GAGCTTCTTA 841 TTGGTGACGT GGAACTGAAA AGGGTGATGG CTTGTTCCAG ATGCATTTTA ACCACAGTGG 901 ACCCAGACAC CGGTGTCATG AGCAGGAAGG AACCGCTGGA AACACTGAAG AGTTATCGCC 961 AGTGTGACCC TTCAGAACGA AAGTTATATG GAAAATCACC ACTCTTTGGG CAGTATTTTG 1021 TGCTGGAAAA CCCAGGGACC ATCAAAGTGG GAGACCCTGT GTACCTGCTG GGCCAGTACC 1081 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1141 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1201 ATATATCTTG TGGAAAGGAC GATAATATAA CGCCTCGTAT CTGATTACGC GTTAAGTCga 1261 caatcaacct ctggattaca aaatttgtga aagatt