Construct: ORF TRCN0000476723
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001939.1_s317c1
- Derived from:
- ccsbBroadEn_13818
- DNA Barcode:
- TGCATGGCATGCCTTTTAGTAAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATP5F1E (514)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476723
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 514 | ATP5F1E | ATP synthase F1 subunit eps... | NM_006886.4 | 100% | 100% | |
| 2 | human | 100533975 | SLMO2-ATP5E | SLMO2-ATP5E readthrough | NR_037930.1 | 18.7% | (many diffs) | |
| 3 | human | 100533975 | SLMO2-ATP5E | SLMO2-ATP5E readthrough | NR_037929.1 | 14% | (many diffs) | |
| 4 | mouse | 67126 | Atp5e | ATP synthase, H+ transporti... | NM_025983.3 | 87.8% | 88.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 222
- ORF end:
- 375
- ORF length:
- 153
- Sequence:
-
1 GGAGGGGTTT TATGCGATGG AGTTTCCCCA CACTGAGTGG GTGGAGACTG AAGTTAGGCC 61 AGCTTGGCAC TTGATGTAAT TCTCCTTGGA ATTTGCCCTT TTTGAGTTTG GATCTTGGTT 121 CATTCTCAAG CCTCAGACAG TGGTTCAAAG TTTTTTTCTT CCATTTCAGG TGTCGTGAGG 181 CTAGCATCGA TTGATCAACA AGTTTGTACA AAAAAGTTGG CATGGTGGCC TACTGGAGAC 241 AGGCTGGACT CAGCTACATC CGATACTCCC AGATCTGTGC AAAAGCAGTG AGAGATGCAC 301 TGAAGACAGA ATTCAAAGCA AATGCTGAGA AGACTTCTGG CAGCAACGTA AAAATTGTGA 361 AAGTAAAGAA GGAATACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 421 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 481 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TGCATGGCAT GCCTTTTAGT 541 AAATACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt