Construct: ORF TRCN0000476734
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015047.1_s317c1
- Derived from:
- ccsbBroadEn_03693
- DNA Barcode:
- TTTCGGCATCGACTTGCTGAAGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NXF5 (55998)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476734
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55998 | NXF5 | nuclear RNA export factor 5 | NM_032946.2 | 100% | 100% | |
| 2 | human | 728343 | NXF2B | nuclear RNA export factor 2B | NM_001099686.2 | 54.7% | 50.9% | (many diffs) |
| 3 | human | 56001 | NXF2 | nuclear RNA export factor 2 | NM_022053.3 | 54.7% | 50.9% | (many diffs) |
| 4 | human | 55998 | NXF5 | nuclear RNA export factor 5 | NR_159739.1 | 53.4% | (many diffs) | |
| 5 | human | 55998 | NXF5 | nuclear RNA export factor 5 | NR_159737.1 | 53.2% | (many diffs) | |
| 6 | human | 55998 | NXF5 | nuclear RNA export factor 5 | NR_028089.1 | 52.8% | (many diffs) | |
| 7 | human | 55998 | NXF5 | nuclear RNA export factor 5 | NR_159738.1 | 50.2% | (many diffs) | |
| 8 | human | 55998 | NXF5 | nuclear RNA export factor 5 | NR_159736.1 | 50.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1164
- ORF length:
- 1095
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaggcggaac acacaagatg aaaacatgag gaaatggttc aaggtcacaa 121 ttccttatgg gataaagtat gacaaggcat ggctaatgaa ttcaatccag agcaattgca 181 gtgtcccctt cactccggtt gatttccact acatccgaaa tcgggcatgc ttctttgtcc 241 aggttgctag tgctgcctct gcattgaagg atgtcagtta taagatttat gatgatgaga 301 accaaaagat atgtatattt gtcagtcatt ttactgcgcc ctactctgtg aagaataagt 361 tgaagccagg ccaaatggag atgctaaagc tgaccatgaa caaacggtac aatgtctccc 421 agcaagctct tgatctccag aatctccgct ttgacccaga cttgatgggc cgtgacattg 481 atataatcct gaatcgaaga aactgcatgg ctgccaccct gaagatcact gaaagaaatt 541 tccctgagct gttgtctttg aacttgtgca acaacaaact gtaccagctg gatggccttt 601 ctgacattac agagaaggct cccaaagtca agaccctgaa tctctccaaa aataagctgg 661 agtcggcgtg ggagttgggc aaggtgaaag ggctgaagct cgaagagcta tggctagaag 721 GGAACCCGTT GTGCAGCACC TTCTCGGACC AGTCTGCCTA TGTAAGTGCC ATCCGGGATT 781 GTTTCCCCAA GTTGTTACGC CTGGACGGCC GAGAGTTATC CGCACCAGTG ATTGTTGACA 841 TTGACAGCTC TGAGACAATG AAACCCTGCA AGGAAAACTT TACTGGATCT GAGACCCTAA 901 AGCATTTAGT CCTGCAATTC CTGCAGCAGA GCAACTTGTG CAAGTACTTC AAGGATAGCA 961 GGAATATAAA AATTCTCAAA GACCCCTACC TGCAGAGGAA GCTGCTAAAG CACACAAAAT 1021 GTCCACGAAA CGTGGACTCC CTCAGTGCAT TGCCTGAAAC TCAGCATGAC TTCACCTCCA 1081 TCCTGGTGGA CATGTGGTAC CAGACCGTGA ACACCTGCTT CCTCCCTCGG GCAGGCCCAG 1141 AGAGCCAGAG CCTAAGACCA CTCTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1201 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1261 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT TTCGGCATCG 1321 ACTTGCTGAA GGCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1381 att