Construct: ORF TRCN0000476762
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012693.1_s317c1
- Derived from:
- ccsbBroadEn_05483
- DNA Barcode:
- CTTCCTGCAACTATTTCTCAGGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NCCRP1 (342897)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476762
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 342897 | NCCRP1 | NCCRP1, F-box associated do... | NM_001001414.2 | 100% | 100% | |
2 | human | 342897 | NCCRP1 | NCCRP1, F-box associated do... | XM_011526906.3 | 69.6% | 68.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 891
- ORF length:
- 825
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggaggtgcgt gagggacacg cgctcggtgg cgggatggaa gccgatgggc 121 ccgcgagcct ccaggagctg cctccctcgc cacggtcgcc ttcaccgccg ccgtcgccgc 181 caccactgcc ctcgccgccg tcgctgccat cgcccgcagc cccggaggcc cccgagctcc 241 ccgagccggc gcagccgtcc gaggctcacg cccggcagct gctgctggag gagtgggggc 301 cgctgagcgg gggcctggag ctgccccagc gcctcacctg gaagctgctc ctgttgcggc 361 ggccgctcta ccgcaacctg ctgcgctcgc ccaaccccga aggcatcaac atttatgagc 421 cagcaccccc tactggtccc acccagcgac ccctggaaac tctgggcaat ttccgtggct 481 ggtacattag aactgaaaag ctccagcaga accaaagctg gacagtgaag cagcagtgtg 541 tggaccTTCT GGCCGAGGGC CTGTGGGAGG AGCTGCTGGA TGACGAACAA CCAGCCATTA 601 CGGTCATGGA CTGGTTCGAG GACAGCCGGC TGGATGCGTG CGTCTATGAG CTGCATGTCT 661 GGCTGCTGGC GGCCGACCGC CGCACGGTCA TTGCTCAGCA CCACGTGGCC CCCCGAACTT 721 CTGGGAGAGG ACCCCCTGGC CGCTGGGTCC AGGTGTCCCA CGTATTCCGC CATTATGGTC 781 CCGGTGTGCG CTTTATCCAC TTCCTGCACA AGGCCAAGAA CCGCATGGAG CCTGGTGGGC 841 TGCGGCGGAC ACGGGTGACC GACTCCTCCG TGTCTGTGCA GCTCCGGGAG TGCCCAACTT 901 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 961 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1021 CTTGTGGAAA GGACGACTTC CTGCAACTAT TTCTCAGGCG ACGCGTTAAG TCgacaatca 1081 acctctggat tacaaaattt gtgaaagatt