Construct: ORF TRCN0000476800

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000994.1_s317c1
Derived from:
ccsbBroadEn_00805
DNA Barcode:
TGTACCCGCGAGTCTCCACACCGT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
HTR2C (3358)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000476800
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3358 HTR2C 5-hydroxytryptamine recepto... NM_000868.3 100% 100%
2 human 3358 HTR2C 5-hydroxytryptamine recepto... NM_001256760.2 100% 100%
3 human 3358 HTR2C 5-hydroxytryptamine recepto... NM_001256761.2 54.1% 37.7% 454_455ins95;744_745ins535
4 mouse 15560 Htr2c 5-hydroxytryptamine (seroto... NM_008312.4 86.2% 90% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1443
ORF length:
1374
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggtgaacctg aggaatgcgg tgcattcatt ccttgtgcac ctaattggcc
121tattggtttg gcaatgtgat atttctgtga gcccagtagc agctatagta actgacattt
181tcaatacctc cgatggtgga cgcttcaaat tcccagacgg ggtacaaaac tggccagcac
241tttcaatcgt catcataata atcatgacaa taggtggcaa catccttgtg atcatggcag
301taagcatgga aaagaaactg cacaatgcca ccaattactt cttaatgtcc ctagccattg
361ctgatatgct agtgggacta cttgtcatgc ccctgtctct cctggcaatc ctttatgatt
421atgtctggcc actacctaga tatttgtgcc ccgtctggat ttctttagat gttttatttt
481caacagcgtc catcatgcac ctctgcgcta tatcgctgga tcggtatgta gcaatacgta
541atcctattga gcatagccgt ttcaattcgc ggactaaggc catcatgaag attgctattg
601tttgggcaat ttctataggt gtatcagttc ctatccctgt gattggactg agggacgaag
661aaaaggtgtt cgtgaacaac acgacgtgcg tgctcaacga cccaaatttc gttcttattg
721ggtccttcgt agctttcttc ataccgctga cgattatggt gattacgtat tgcctgacca
781tctacgttct gcgccgacaa gctttgatgt tactgcacgg ccacaccgag gaaccgcctg
841gactaagtct ggatttcctg aagtgctgca agaggaatac ggccgaggaa gagaactctg
901caaaccctaa ccaagaccag aacgcacgcc gaagaaagaa gaaggagaga cgtcctaggg
961gcaccatgca ggctatcaac aatgaaagaa aagcttcgaa agtccttggg attgttttct
1021ttgtgtttct gatcatgtgg tgcccatttt tcattaccaa tattctgtct gttctttgtg
1081agaagtcctg taaccaaaag ctcatggaaa agcttctgaa tgtgtttgtt tggattGGCT
1141ATGTTTGTTC AGGAATCAAT CCTCTGGTGT ATACTCTGTT CAACAAAATT TACCGAAGGG
1201CATTCTCCAA CTATTTGCGT TGCAATTATA AGGTAGAGAA AAAGCCTCCT GTCAGGCAGA
1261TTCCAAGAGT TGCCGCCACT GCTTTGTCTG GGAGGGAGCT TAATGTTAAC ATTTATCGGC
1321ATACCAATGA ACCGGTGATC GAGAAAGCCA GTGACAATGA GCCCGGTATA GAGATGCAAG
1381TTGAGAATTT AGAGTTACCA GTAAATCCCT CCAGTGTGGT TAGCGAAAGG ATTAGCAGTG
1441TGTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC
1501TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT
1561GGCTTTATAT ATCTTGTGGA AAGGACGATG TACCCGCGAG TCTCCACACC GTACGCGTTA
1621AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt

Download FASTA (ORF) (Full)