Construct: ORF TRCN0000476800
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000994.1_s317c1
- Derived from:
- ccsbBroadEn_00805
- DNA Barcode:
- TGTACCCGCGAGTCTCCACACCGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HTR2C (3358)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476800
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3358 | HTR2C | 5-hydroxytryptamine recepto... | NM_000868.3 | 100% | 100% | |
| 2 | human | 3358 | HTR2C | 5-hydroxytryptamine recepto... | NM_001256760.2 | 100% | 100% | |
| 3 | human | 3358 | HTR2C | 5-hydroxytryptamine recepto... | NM_001256761.2 | 54.1% | 37.7% | 454_455ins95;744_745ins535 |
| 4 | mouse | 15560 | Htr2c | 5-hydroxytryptamine (seroto... | NM_008312.4 | 86.2% | 90% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1443
- ORF length:
- 1374
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtgaacctg aggaatgcgg tgcattcatt ccttgtgcac ctaattggcc 121 tattggtttg gcaatgtgat atttctgtga gcccagtagc agctatagta actgacattt 181 tcaatacctc cgatggtgga cgcttcaaat tcccagacgg ggtacaaaac tggccagcac 241 tttcaatcgt catcataata atcatgacaa taggtggcaa catccttgtg atcatggcag 301 taagcatgga aaagaaactg cacaatgcca ccaattactt cttaatgtcc ctagccattg 361 ctgatatgct agtgggacta cttgtcatgc ccctgtctct cctggcaatc ctttatgatt 421 atgtctggcc actacctaga tatttgtgcc ccgtctggat ttctttagat gttttatttt 481 caacagcgtc catcatgcac ctctgcgcta tatcgctgga tcggtatgta gcaatacgta 541 atcctattga gcatagccgt ttcaattcgc ggactaaggc catcatgaag attgctattg 601 tttgggcaat ttctataggt gtatcagttc ctatccctgt gattggactg agggacgaag 661 aaaaggtgtt cgtgaacaac acgacgtgcg tgctcaacga cccaaatttc gttcttattg 721 ggtccttcgt agctttcttc ataccgctga cgattatggt gattacgtat tgcctgacca 781 tctacgttct gcgccgacaa gctttgatgt tactgcacgg ccacaccgag gaaccgcctg 841 gactaagtct ggatttcctg aagtgctgca agaggaatac ggccgaggaa gagaactctg 901 caaaccctaa ccaagaccag aacgcacgcc gaagaaagaa gaaggagaga cgtcctaggg 961 gcaccatgca ggctatcaac aatgaaagaa aagcttcgaa agtccttggg attgttttct 1021 ttgtgtttct gatcatgtgg tgcccatttt tcattaccaa tattctgtct gttctttgtg 1081 agaagtcctg taaccaaaag ctcatggaaa agcttctgaa tgtgtttgtt tggattGGCT 1141 ATGTTTGTTC AGGAATCAAT CCTCTGGTGT ATACTCTGTT CAACAAAATT TACCGAAGGG 1201 CATTCTCCAA CTATTTGCGT TGCAATTATA AGGTAGAGAA AAAGCCTCCT GTCAGGCAGA 1261 TTCCAAGAGT TGCCGCCACT GCTTTGTCTG GGAGGGAGCT TAATGTTAAC ATTTATCGGC 1321 ATACCAATGA ACCGGTGATC GAGAAAGCCA GTGACAATGA GCCCGGTATA GAGATGCAAG 1381 TTGAGAATTT AGAGTTACCA GTAAATCCCT CCAGTGTGGT TAGCGAAAGG ATTAGCAGTG 1441 TGTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1501 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1561 GGCTTTATAT ATCTTGTGGA AAGGACGATG TACCCGCGAG TCTCCACACC GTACGCGTTA 1621 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt