Transcript: Human NM_000868.3

Homo sapiens 5-hydroxytryptamine receptor 2C (HTR2C), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-08-06
Taxon:
Homo sapiens (human)
Gene:
HTR2C (3358)
Length:
4774
CDS:
729..2105

Additional Resources:

NCBI RefSeq record:
NM_000868.3
NBCI Gene record:
HTR2C (3358)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_000868.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000378358 CTATTTGCGTTGCAATTATAA pLKO_005 1871 CDS 100% 15.000 21.000 N HTR2C n/a
2 TRCN0000357445 CTTCATACCGCTGACGATTAT pLKO_005 1397 CDS 100% 13.200 18.480 N HTR2C n/a
3 TRCN0000009099 CCCGGTATAGAGATGCAAGTT pLKO.1 2022 CDS 100% 4.950 6.930 N HTR2C n/a
4 TRCN0000009102 CCGCTGACGATTATGGTGATT pLKO.1 1404 CDS 100% 4.950 6.930 N HTR2C n/a
5 TRCN0000009101 CCGTTTCAATTCGCGGACTAA pLKO.1 1217 CDS 100% 4.950 6.930 N HTR2C n/a
6 TRCN0000009100 GCACTTTCAATCGTCATCATA pLKO.1 897 CDS 100% 5.625 3.938 N HTR2C n/a
7 TRCN0000009098 GCAGTTATTTACCAGAAGTTT pLKO.1 3359 3UTR 100% 5.625 3.938 N HTR2C n/a
8 TRCN0000378251 TCATGCACCTCTGCGCTATAT pLKO_005 1153 CDS 100% 13.200 7.920 N HTR2C n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_000868.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00805 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_00805 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000476800 TGTACCCGCGAGTCTCCACACCGT pLX_317 22.6% 100% 100% V5 n/a
4 TRCN0000488530 GGTTCAATCAACCTAATCCATCAT pLX_317 22.8% 100% 100% V5 (not translated due to prior stop codon) n/a
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