Construct: ORF TRCN0000476801
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009737.1_s317c1
- Derived from:
- ccsbBroadEn_00764
- DNA Barcode:
- TTGGTATAAGCAGGCAATCCGTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ONECUT1 (3175)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476801
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3175 | ONECUT1 | one cut homeobox 1 | NM_004498.4 | 100% | 100% | |
| 2 | human | 3175 | ONECUT1 | one cut homeobox 1 | XM_011521487.2 | 83.4% | 79.4% | (many diffs) |
| 3 | human | 3175 | ONECUT1 | one cut homeobox 1 | XR_001751239.1 | 54.1% | (many diffs) | |
| 4 | human | 3175 | ONECUT1 | one cut homeobox 1 | XR_931800.2 | 50.1% | (many diffs) | |
| 5 | mouse | 15379 | Onecut1 | one cut domain, family memb... | NM_008262.3 | 93.8% | 98.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1464
- ORF length:
- 1395
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaacgcgcag ctgaccatgg aagcgatcgg cgagctgcac ggggtgagcc 121 atgagccggt gcccgcccct gccgacctgc tgggcggcag cccccacgcg cgcagctccg 181 tggcgcaccg cggcagccac ctgccccccg cgcacccgcg ctccatgggc atggcgtccc 241 tgctggacgg cggcagcggc ggcggagatt accaccacca ccaccgggcc cctgagcaca 301 gcctggccgg ccccctgcat cccaccatga ccatggcctg cgagactccc ccaggtatga 361 gcatgcccac cacctacacc accttgaccc ctctgcagcc gctgcctccc atctccacag 421 tctcggacaa gttcccccac catcaccacc accaccatca ccaccaccac ccgcaccacc 481 accagcgcct ggcgggcaac gtgagcggta gcttcacgct catgcgggat gagcgcgggc 541 tggcctccat gaataacctc tataccccct accacaagga cgtggccggc atgggccaga 601 gcctctcgcc cctctccagc tccggtctgg gcagcatcca caactcccag caagggctcc 661 cccactatgc ccacccgggg gccgccatgc ccaccgacaa gatgctcacc cccaacggct 721 tcgaagccca ccacccggcc atgctcggcc gccacgggga gcagcacctc acgcccacct 781 cggccggcat ggtgcccatc aacggccttc ctccgcacca tccccacgcc cacctgaacg 841 cccagggcca cgggcaactc ctgggcacag cccgggagcc caacccttcg gtgaccggcg 901 cgcaggtcag caatggaagt aattcagggc agatggaaga gatcaatacc aaagaggtgg 961 cgcagcgtat caccaccgag ctcaagcgct acagcatccc acaggccatc ttcgcgcaga 1021 gggtgctctg ccgctcccag gggaccctct cggacctgct gcgcaacccc aaaccctgga 1081 gcaaactcaa atccggccgg gagaccTTCC GGAGGATGTG GAAGTGGCTG CAGGAGCCGG 1141 AGTTCCAGCG CATGTCCGCG CTCCGCTTAG CAGCATGCAA AAGGAAAGAA CAAGAACATG 1201 GGAAGGATAG AGGCAACACA CCCAAAAAGC CCAGGTTGGT CTTCACAGAT GTCCAGCGTC 1261 GAACTCTACA TGCAATATTC AAGGAAAATA AGCGTCCATC CAAAGAATTG CAAATCACCA 1321 TTTCCCAGCA GCTGGGGTTG GAGCTGAGCA CTGTCAGCAA CTTCTTCATG AACGCAAGAA 1381 GGAGGAGTCT GGACAAGTGG CAGGACGAGG GCAGCTCCAA TTCAGGCAAC TCATCTTCTT 1441 CATCAAGCAC TTGTACCAAA GCATTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1501 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1561 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT TGGTATAAGC 1621 AGGCAATCCG TTAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1681 att