Construct: ORF TRCN0000476809
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000136.1_s317c1
- Derived from:
- ccsbBroadEn_07271
- DNA Barcode:
- TCCAACACAGCGAGCCTCTTGGCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TNFSF11 (8600)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476809
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8600 | TNFSF11 | TNF superfamily member 11 | NM_003701.4 | 99.8% | 100% | 126T>C |
| 2 | human | 8600 | TNFSF11 | TNF superfamily member 11 | XM_017020802.1 | 78.4% | 74.7% | (many diffs) |
| 3 | human | 8600 | TNFSF11 | TNF superfamily member 11 | NM_033012.3 | 76.9% | 76.9% | 0_1ins219 |
| 4 | human | 8600 | TNFSF11 | TNF superfamily member 11 | XM_011535280.2 | 76.9% | 76.9% | 0_1ins219 |
| 5 | human | 8600 | TNFSF11 | TNF superfamily member 11 | XM_017020803.2 | 76.9% | 76.9% | 0_1ins219 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1020
- ORF length:
- 951
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcgccgcgcc agcagagact acaccaagta cctgcgtggc tcggaggaga 121 tgggcggcgg ccccggagcc ccgcacgagg gccccctgca cgccccgccg ccgcctgcgc 181 cgcaccagcc ccccgccgcc tcccgctcca tgttcgtggc cctcctgggg ctggggctgg 241 gccaggttgt ctgcagcgtc gccctgttct tctatttcag agcgcagatg gatcctaata 301 gaatatcaga agatggcact cactgcattt atagaatttt gagactccat gaaaatgcag 361 attttcaaga cacaactctg gagagtcaag atacaaaatt aatacctgat tcatgtagga 421 gaattaaaca ggcctttcaa ggagctgtgc aaaaggaatt acaacatatc gttggatcac 481 agcacatcag agcagagaaa gcgatggtgg atggctcatg gttagatctg gccaagagga 541 gcaagcttga agctcagcct tttgctcatc tcactattaa tgccaccgac atcccatctg 601 gttcccataa agtgagtctg tcctcttggt accatgatcg gggttgggcc aagaTCTCCA 661 ACATGACTTT TAGCAATGGA AAACTAATAG TTAATCAGGA TGGCTTTTAT TACCTGTATG 721 CCAACATTTG CTTTCGACAT CATGAAACTT CAGGAGACCT AGCTACAGAG TATCTTCAAC 781 TAATGGTGTA CGTCACTAAA ACCAGCATCA AAATCCCAAG TTCTCATACC CTGATGAAAG 841 GAGGAAGCAC CAAGTATTGG TCAGGGAATT CTGAATTCCA TTTTTATTCC ATAAACGTTG 901 GTGGATTTTT TAAGTTACGG TCTGGAGAGG AAATCAGCAT CGAGGTCTCC AACCCCTCCT 961 TACTGGATCC GGATCAGGAT GCAACATACT TTGGGGCTTT TAAAGTTCGA GATATAGATT 1021 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1081 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1141 TTTATATATC TTGTGGAAAG GACGATCCAA CACAGCGAGC CTCTTGGCTA CGCGTTAAGT 1201 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt