Construct: ORF TRCN0000476824
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014991.1_s317c1
- Derived from:
- ccsbBroadEn_09362
- DNA Barcode:
- GGTGGCCTATATCCGCTTCCATTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARHGAP18 (93663)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476824
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 93663 | ARHGAP18 | Rho GTPase activating prote... | NM_033515.3 | 99.9% | 99.8% | 67A>G |
| 2 | human | 93663 | ARHGAP18 | Rho GTPase activating prote... | XM_005267212.2 | 90.6% | 88.1% | (many diffs) |
| 3 | human | 93663 | ARHGAP18 | Rho GTPase activating prote... | XM_005267213.1 | 88.1% | 85.9% | 67A>G;1713_1714ins125;1755_1756ins109 |
| 4 | human | 93663 | ARHGAP18 | Rho GTPase activating prote... | XM_017011494.1 | 79.8% | 75.2% | (many diffs) |
| 5 | mouse | 73910 | Arhgap18 | Rho GTPase activating prote... | NM_176837.2 | 84.1% | 84.5% | (many diffs) |
| 6 | mouse | 73910 | Arhgap18 | Rho GTPase activating prote... | XR_001779622.1 | 40.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2058
- ORF length:
- 1989
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagctggctc tccagttccc agggagtggt actaacagcc taccacccca 121 gcggcaagga ccaggccgtc gggaacagcc atgcaaaggc aggggaggaa gccacctcga 181 gtcgcagata tggccagtac actatgaacc aggaaagcac caccatcaaa gttatggaga 241 agcctccatt tgatcgatca atttcccagg attctttgga tgaactatct atggaagact 301 attggataga actagaaaac atcaagaaat ctagtgaaaa cagccaagaa gatcaagagg 361 tggttgttgt caaagagcct gatgagggag aattggaaga agagtggctt aaagaggccg 421 gtttatccaa tctcttcgga gagtctgctg gagatccaca ggaaagcatt gtgtttttat 481 caacattgac gcggacccag gcagcagcag ttcagaagcg agtagagacg gtctcccaga 541 ccttgaggaa aaaaaacaaa cagtaccaga ttcctgacgt cagagacata tttgctcaac 601 agagagaatc aaaagaaaca gctccaggtg gcactgaatc gcagtcactt agaacaaatg 661 aaaacaaata ccaaggaaga gatgacgagg catctaacct tgttggtgaa gagaagctga 721 tcccacctga ggagacgcct gcccctgaaa cagacatcaa cctggaggta tcatttgccg 781 agcaagcact caatcagaaa gagagctcca aggagaaaat ccagaagagc aaaggcgatg 841 atgccacatt acctagtttc agattgccaa aagacaaaac gggtaccaca aggattggtg 901 acctcgcacc ccaggacatg aagaaagttt gccatttagc cctaattgag ctgactgccc 961 tctatgatgt attgggtatt gagctgaaac aacaaaaagc tgtgaaaatc aaaacaaaag 1021 attctggtct tttttgcgtt ccattgacag cgctattaga acaagatcag aggaaagtac 1081 caggaatgcg aatacccttg atctttcaaa aactgatttc tcgaattgaa gagagaggtt 1141 tggaaacaga aggcctctta cggatccctg gagctgccat tagaatcaag aatctttgcc 1201 aagaactaga agcaaagttt tatgaaggga cttttaattg ggaaagtgtc aaacagcatg 1261 atgccgccag cctgctgaag ctcttcattc gggagttgcc ccagccactg ctcagtgtgg 1321 agtatctcaa agcctttcag gctgtccaga atcttccaac caagaagcag caactacagg 1381 ctttgaacct tcttgtcatc ctcctacctg atgcaaacag ggacacactg aaggcccttc 1441 ttgaatttct ccaaagagta atagataata aagaaaaaaa taaaatgaca gtcatgaatg 1501 tagcaatggt catggccccg aatctcttta tgtgtcatgc attgggattg aagtccagtg 1561 aacagcgaga atttgtaatg gcagctggga cagcaaatac catgcactta ttgattaagt 1621 accaaaaact tctgtgGACA ATTCCCAAGT TTATTGTAAA CCAAGTGAGG AAGCAAAACA 1681 CGGAAAATCA TAAAAAGGAT AAAAGAGCCA TGAAGAAATT GCTGAAGAAA ATGGCTTATG 1741 ACCGAGAAAA ATATGAAAAG CAAGATAAGA GTACAAATGA TGCTGACGTT CCTCAGGGAG 1801 TGATTCGAGT GCAAGCTCCC CATCTTTCGA AAGTTTCCAT GGCAATACAG CTAACTGAAG 1861 AACTAAAAGC CAGTGATGTA CTTGCCAGGT TTCTCAGCCA AGAAAGTGGG GTTGCCCAGA 1921 CTCTCAAGAA AGGAGAAGTT TTTTTGTATG AAATTGGAGG AAATATTGGG GAACGCTGCC 1981 TTGATGATGA CACTTACATG AAGGATTTAT ATCAGCTTAA CCCAAATGCT GAGTGGGTTA 2041 TAAAGTCAAA GCCATTGTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 2101 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 2161 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGGTGGCC TATATCCGCT 2221 TCCATTGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt