Construct: ORF TRCN0000476836

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012137.1_s317c1
Derived from:
ccsbBroadEn_15318
DNA Barcode:
GCGCCCCAGACGGGGGGTCTCTCT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
KCTD21-AS1 (100289388)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000476836
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 100289388 KCTD21-AS1 KCTD21 antisense RNA 1 NR_102281.1 14.8% 1_400del;555_556ins490
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
711
ORF length:
645
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtg ggtgagaaga atcgatgcag aagccatcgc tcagagcgat tttcagtacc
121tcttccacga agacctggaa gctgttgatc tgcttgttgg cgggcaccac ccagagcctc
181ttgaggttct gcttggtgta ctcctcctct ggcaggccct ccacattggc cacccagtcc
241agagtcaggt ggttggggtc ctgcaagtgg ctggtgatgg aggagagatt gccattggag
301cagtagaaga gcttagagcc aaggagcttg aggaagaggc aggaggtgct gaagatgttg
361gcgttgaaga ccTCCATGCT GGAAGAGGAG AGGCTGTAGA TCTGGGGTGC CTCGCGCACA
421GTGAAGTGGA CCGTCTGCAC ACGCTGGTTC AGTGTGATGT TGAGCATGGC ATTCTTCTCG
481GCCTTGGAGA GCTCCACTTC CTTCTCCTGC AGGGCCTCAA TCAGGGGCTG CACCTGGTAG
541AAGTCGGCCT CCCTGCGGAG CAGCCCCATC TCCTGGAAGT CCTCAGGCAG GTCAAGGTGG
601GAGGTCCGCA GGAAGTTGAG GATATAGCGG AACACTTTGC CGTCACGGTC AATGAAGCAG
661TTGCCCTGGC TGTCCCTCTT GGTGGGCATC TTCCCGCTGA ACATGGCGCC TACCCAACTT
721TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
781CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
841CTTGTGGAAA GGACGAGCGC CCCAGACGGG GGGTCTCTCT ACGCGTTAAG TCgacaatca
901acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)