Construct: ORF TRCN0000476836
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012137.1_s317c1
- Derived from:
- ccsbBroadEn_15318
- DNA Barcode:
- GCGCCCCAGACGGGGGGTCTCTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KCTD21-AS1 (100289388)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476836
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 100289388 | KCTD21-AS1 | KCTD21 antisense RNA 1 | NR_102281.1 | 14.8% | 1_400del;555_556ins490 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 711
- ORF length:
- 645
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg ggtgagaaga atcgatgcag aagccatcgc tcagagcgat tttcagtacc 121 tcttccacga agacctggaa gctgttgatc tgcttgttgg cgggcaccac ccagagcctc 181 ttgaggttct gcttggtgta ctcctcctct ggcaggccct ccacattggc cacccagtcc 241 agagtcaggt ggttggggtc ctgcaagtgg ctggtgatgg aggagagatt gccattggag 301 cagtagaaga gcttagagcc aaggagcttg aggaagaggc aggaggtgct gaagatgttg 361 gcgttgaaga ccTCCATGCT GGAAGAGGAG AGGCTGTAGA TCTGGGGTGC CTCGCGCACA 421 GTGAAGTGGA CCGTCTGCAC ACGCTGGTTC AGTGTGATGT TGAGCATGGC ATTCTTCTCG 481 GCCTTGGAGA GCTCCACTTC CTTCTCCTGC AGGGCCTCAA TCAGGGGCTG CACCTGGTAG 541 AAGTCGGCCT CCCTGCGGAG CAGCCCCATC TCCTGGAAGT CCTCAGGCAG GTCAAGGTGG 601 GAGGTCCGCA GGAAGTTGAG GATATAGCGG AACACTTTGC CGTCACGGTC AATGAAGCAG 661 TTGCCCTGGC TGTCCCTCTT GGTGGGCATC TTCCCGCTGA ACATGGCGCC TACCCAACTT 721 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 781 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 841 CTTGTGGAAA GGACGAGCGC CCCAGACGGG GGGTCTCTCT ACGCGTTAAG TCgacaatca 901 acctctggat tacaaaattt gtgaaagatt