Construct: ORF TRCN0000476864
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013078.1_s317c1
- Derived from:
- ccsbBroadEn_08146
- DNA Barcode:
- TGTTTCCGTTCTGCTCTACGGATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PDZRN4 (29951)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476864
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29951 | PDZRN4 | PDZ domain containing ring ... | NM_013377.4 | 99.9% | 99.8% | 2329A>C |
| 2 | human | 29951 | PDZRN4 | PDZ domain containing ring ... | NM_001164595.2 | 74.6% | 72.3% | (many diffs) |
| 3 | mouse | 239618 | Pdzrn4 | PDZ domain containing RING ... | NM_001164594.1 | 85.1% | 88.4% | (many diffs) |
| 4 | mouse | 239618 | Pdzrn4 | PDZ domain containing RING ... | NM_001164593.1 | 64.2% | 65.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2403
- ORF length:
- 2334
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggctgcaat ttgtgcactt ttcagaaaag agaggaacac tacaaactgc 121 tgtatgaagt ttcccaggtc aatgggaagg atctttcaaa ggccactcat gaagaggcag 181 tggaagcttt tcgcaatgcc aaggagccca ttgtggtgca ggtgttaagg cgaacacctc 241 ttagtagacc agcctatggg atggcttcag aagtgcagct tatgaatgcc agcactcaga 301 cggacatcac cttcgaacac atcatggctc tggccaagct tcgtccacct acccctccag 361 tgccagacat ctgtccattc ctgctctcag acagctgcca ttctctacat ccaatggagc 421 atgaatttta tgaggacaat gagtatattt ccagcttgcc tgctgatgca gacagaacag 481 aagactttga atatgaggag gtcgagttgt gtcgtgttag cagtcaagag aagctgggcc 541 tgacagtctg ttaccgaaca gatgatgaag aagacaccgg catttatgtc agcgaggttg 601 acccaaatag cattgctgcc aaagacggcc ggattcgaga aggggatcgg attttgcaaa 661 taaatgggga agatgtccag aatcgagaag aagcagtggc cttgctgtct aacgatgagt 721 gtaagagaat cgtgctgctt gttgcaaggc cagagattca gctggatgaa ggctggctgg 781 aagatgaaag gaatgaattc ttagaggagt taaacttgga gatgttggaa gaagagcata 841 atgaagcaat gcagcccact gccaatgagg tggagcagcc aaaaaagcaa gaagaagaag 901 aaggcacaac agacactgca acatcctcat ccaacaacca tgagaaggac agtggagtag 961 gacgtacaga tgaaagcttg cgaaatgatg agagctcaga gcaggagaat gcagccgagg 1021 accccaatag cacatctttg aagagcaaga gagacctggg gcagagccaa gacactctgg 1081 gaagtgttga acttcagtac aatgagagcc tcgtatctgg tgaatacatt gactcagact 1141 gcattggcaa cccagatgag gactgtgaaa gattcaggca gctcttggag ctcaaatgca 1201 agattcgaaa tcatggagag tatgacctgt attactcaag cagcacaatt gaatgcaatc 1261 aaggggagca agagggagtg gagcatgagc tacagttgct taatgaagaa ctgagaaaca 1321 ttgagcttga gtgtcagaat atcatgcagg ctcacaggct ccagaaagtg acagaccagt 1381 atggagacat ctggacattg catgatggag gattccggaa ttataacacc agcatagata 1441 tgcaaagggg aaagctagat gacatcatgg agcatccaga aaagtctgac aaggacagtt 1501 ctagtgctta caacacagct gagagctgca gaagtactcc gctcactgta gaccgttccc 1561 ctgacagttc ccttccaagg gtgatcaacc tcaccaataa gaaaaacctg agaagcacaa 1621 tggcagccac ccagtcctct tccggacaga gcagtaaaga gtcgacctcc accaaagcca 1681 aaaccactga gcaaggttgt agcgctgaaa gcaaggagaa ggttttagaa ggcagcaagc 1741 ttcctgatca agagaaggca gtcagcgaac acatccctta cctctctcct taccacagct 1801 cctcatatag atatgcaaac atcccagcac acgcccggca ttatcaaagc tacatgcagt 1861 taattcaaca gaaatctgca gtcgagtatg ctcagagtca gctcagcttg gtgagcatgt 1921 gcaaggagtc tcagaagtgt tcagagccca agatggaatg gaaggtgaaa attaggagcg 1981 acgggacacg gtacatcaca aagagacccg tgcgagaccg aatcctgaag gaacgtgccT 2041 TAAAGATCAA GGAAGAGCGG AGTGGCATGA CCACAGACGA TGACACCATG AGCGAGATGA 2101 AAATGGGGCG CTACTGGAGC AAAGAGGAGA GAAAGCAGCA CCTGGTTAGG GCCAAAGAGC 2161 AGCGCCGTCG CCGTGAGTTC ATGATGCGAA GCAGGTTAGA GTGTCTCAAG GAGAGCCCTC 2221 AGAGCGGCAG TGAGGGCAAG AAGGAGATCA ATATCATTGA ACTGAGTCAC AAAAAGATGA 2281 TGAAAAAGAG AAACAAGAAA ATTTTGGACA ACTGGATGAC AATCCAAGAA CTGATGACCC 2341 ATGGGGCCAA GTCTCCAGAT GGCACGAGAG TCCATAATGC CTTCTTGTCG GTGACCCCTG 2401 TATTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 2461 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 2521 GGCTTTATAT ATCTTGTGGA AAGGACGATG TTTCCGTTCT GCTCTACGGA TAACGCGTTA 2581 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt