Construct: ORF TRCN0000476869
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017991.1_s317c1
- Derived from:
- ccsbBroadEn_14402
- DNA Barcode:
- AGCCTGGATCTCGCGAGAATCGCG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- SAMD11 (148398)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476869
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 148398 | SAMD11 | sterile alpha motif domain ... | NM_152486.3 | 72.7% | 39% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 870
- ORF length:
- 804
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cccggaggac cattaccgcc ggcttgtgtc agcactgagc gaggccagca 121 cctttgagga ccctcagcgc ctctaccacc tgggcctccc cagccacggt gaggacccac 181 cctggcatga tccccctcat cacctcccca gccacgatct cctgagggtc cggcaggagg 241 tggcggctgc agctctgagg ggccccagtg gcctggaagc ccacctgccc tcctccacgg 301 caggtcagcg tcggaagcag ggcctggctc agcaccggga gggcgccgcc ccagctgccg 361 ccccgtcctt ctcggagagg gagctgcctc agccgccccc cttgctgtcg ccgcagaatg 421 cccctcacgt cgccctgggc ccccatctca ggcccccctt cctgggggtg ccctcggctc 481 tgtgccagac cccaggctac ggcttcctgc cccccgcgca ggcggagatg ttcgcccggc 541 agcaggagct cctgcggaag cagaacctgg cccggctgga gctgcccgcc gacctcctgc 601 ggcagaagga gctggagagc gcgcgcccac agctgctggc gcccgagacc gccctgcgcc 661 ccaacgacgg cgccgaggag ctgcagcggc gcggggccct gctggtgctg aaccacggcg 721 cggcgccact gctggccctg cccccccagg ggcccccggg ctccggaccc cccaccccgt 781 cccgggactc tgcccggcga gccccccgga aggggggtcc cggccctgcc tcagcgcggc 841 ccagcgagtc caaggagatg gacgggggct aggctctggg cacaagatgg ctcggaagac 901 gagccccccc aaagactcgg acggagagcg accccgagac ggcagctgtt gggtgcaggg 961 ggcccactcc gggccaagct ccagctggag gggccggcgc cgaggggaag gggcttttcc 1021 cagggtccac actgcccctg ggcttccctt atgccgtcag cccctacttc cacacaggcg 1081 cggtaggggg actctccatg gatggggagg aggccccagc ccctgaggac gtcaccaagt 1141 ggaccgtgga tgacgtctgc agcttcgtgg ggggcctgtc tggctgtgga gagtacactc 1201 gggtcttcag ggagcagggg atcgacgggg agaccctgcc actgctgacg gaggagcacc 1261 TGCTGACCAA CATGGGGCTG AAGCTGGGGC CCGCCCTCAA GATCCGGGCC CAGGTGGCCA 1321 GGCGCCTGGG CCGAGTTTTC TACGTGGCCA GCTTCCCCGT GGCTCTGCCA CTGCAGCCAC 1381 CAACCCTGCG GGCCCCGGAG CGAGAACTCG GCACAGGAGA GCAGCCCTTG TCCCCCACGA 1441 CGGCCACGTC CCCCTATGGA GGGGGCCACG CCCTTGCCGG TCAAACTTCA CCCAAGCAGG 1501 AGAATGGGAC CTTGGCTCTA CTTCCAGGGG CCCCCGACCC TTCCCAGCCT CTGTGTTGCC 1561 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1621 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1681 ATATATCTTG TGGAAAGGAC GAAGCCTGGA TCTCGCGAGA ATCGCGACGC GTTAAGTCga 1741 caatcaacct ctggattaca aaatttgtga aagatt