Construct: ORF TRCN0000476876
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017998.1_s317c1
- Derived from:
- ccsbBroadEn_03859
- DNA Barcode:
- ATCCCTTATATTTGTTTGCCTTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ENOPH1 (58478)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476876
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 58478 | ENOPH1 | enolase-phosphatase 1 | NM_021204.5 | 100% | 100% | |
| 2 | human | 58478 | ENOPH1 | enolase-phosphatase 1 | NM_001292017.2 | 66.2% | 66.2% | 0_1ins264 |
| 3 | human | 58478 | ENOPH1 | enolase-phosphatase 1 | NR_120457.2 | 26.4% | (many diffs) | |
| 4 | mouse | 67870 | Enoph1 | enolase-phosphatase 1 | NM_001163035.1 | 84.4% | 93.1% | (many diffs) |
| 5 | mouse | 67870 | Enoph1 | enolase-phosphatase 1 | NM_026421.3 | 84.4% | 93.1% | (many diffs) |
| 6 | mouse | 67870 | Enoph1 | enolase-phosphatase 1 | XM_006535189.2 | 80.9% | 88.1% | (many diffs) |
| 7 | mouse | 67870 | Enoph1 | enolase-phosphatase 1 | XM_006535190.2 | 80.9% | 88.1% | (many diffs) |
| 8 | mouse | 67870 | Enoph1 | enolase-phosphatase 1 | XM_011249550.2 | 80.9% | 88.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 852
- ORF length:
- 783
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtcgtgctt tcggtccccg ccgaagtcac cgtgatcctg ttagatatcg 121 aaggtaccac aaccccgatt gctttcgtga aggacatttt atttccttac atcgaagaaa 181 atgttaaaga gtatctgcag acacattggg aagaagagga gtgccagcag gatgtcagtc 241 ttttgaggaa acaggctgaa gaggacgccc acctggatgg ggctgttcct atccctgcag 301 catctgggaa tggagtggat gatctgcaac agatgatcca ggccgtggta gataatgtgt 361 gctggcagat gtccctggat cgaaagacca ctgcactcaa acagctgcag ggccacatgt 421 ggagggcggc attcacagct gggcgcatga aagcagagtt ctttGCAGAT GTAGTTCCAG 481 CAGTCAGGAA GTGGAGAGAG GCCGGAATGA AGGTGTACAT CTATTCCTCA GGGAGTGTGG 541 AGGCACAGAA ACTGTTATTC GGGCATTCTA CGGAGGGAGA TATTCTTGAG CTTGTTGATG 601 GTCACTTTGA TACCAAGATT GGACACAAAG TAGAGAGTGA AAGTTACCGA AAGATTGCAG 661 ACAGCATTGG GTGCTCAACC AACAACATTT TGTTTCTGAC AGATGTTACT CGAGAGGCCA 721 GTGCTGCTGA GGAAGCAGAT GTGCACGTAG CTGTGGTGGT GAGACCAGGC AACGCAGGAT 781 TAACAGATGA TGAGAAGACT TACTACAGCC TCATCACATC CTTCAGTGAA CTATACCTGC 841 CTTCCTCAAC CTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 901 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 961 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAATC CCTTATATTT GTTTGCCTTC 1021 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t