Construct: ORF TRCN0000476891
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006281.1_s317c1
- Derived from:
- ccsbBroadEn_02895
- DNA Barcode:
- TCCTCGACTCAAAGTGTCATTTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EGFL6 (25975)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476891
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25975 | EGFL6 | EGF like domain multiple 6 | NM_015507.4 | 99.9% | 99.8% | 1648T>C |
| 2 | human | 25975 | EGFL6 | EGF like domain multiple 6 | NM_001167890.2 | 99.7% | 99.6% | 1182_1184delAGC;1651T>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1725
- ORF length:
- 1659
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc tctgccctgg agccttgcgc tcccgctgct gctctcctgg gtggcaggtg 121 gtttcgggaa cgcggccagt gcaaggcatc acgggttgtt agcatcggca cgtcagcctg 181 gggtctgtca ctatggaact aaactggcct gctgctacgg ctggagaaga aacagcaagg 241 gagtctgtga agctacatgc gaacctggat gtaagtttgg tgagtgcgtg ggaccaaaca 301 aatgcagatg ctttccagga tacaccggga aaacctgcag tcaagatgtg aatgagtgtg 361 gaatgaaacc ccggccatgc caacacagat gtgtgaatac acacggaagc tacaagtgct 421 tttgcctcag tggccacatg ctcatgccag atgctacgtg tgtgaactct aggacatgtg 481 ccatgataaa ctgtcagtac agctgtgaag acacagaaga agggccacag tgcctgtgtc 541 catcctcagg actccgcctg gccccaaatg gaagagactg tctagatatt gatgaatgtg 601 cctctggtaa agtcatctgt ccctacaatc gaagatgtgt gaacacattt ggaagctact 661 actgcaaatg tcacattggt ttcgaactgc aatatatcag tggacgatat gactgtatag 721 atataaatga atgtactatg gatagccata cgtgcagcca ccatgccaat tgcttcaata 781 cccaagggtc cttcaagtgt aaatgcaagc agggatataa aggcaatgga cttcggtgtt 841 ctgctatccc tgaaaattct gtgaaggaag tcctcagagc acctggtacc atcaaagaca 901 gaatcaagaa gttgcttgct cacaaaaaca gcatgaaaaa gaaggcaaaa attaaaaatg 961 ttaccccaga acccaccagg actcctaccc ctaaggtgaa cttgcagccc ttcaactatg 1021 aagagatagt ttccagaggc gggaactctc atggaggtaa aaaagggaat gaagagaaaa 1081 tgaaagaggg gcttgaggat gagaaaagag aagagaaagc cctgaagaat gacatagagg 1141 agcgaagcct gcgaggagat gtgtttttcc ctaaggtgaa tgaagcaggt gaattcggcc 1201 tgattctggt ccaaaggaaa gcgctaactt ccaaactgga acataaagat ttaaatatct 1261 cggttgactg cagcttcaat catgggatct gtgactggaa acaggataga gaagatgatt 1321 ttgactggaa tccTGCTGAT CGAGATAATG CTATTGGCTT CTATATGGCA GTTCCGGCCT 1381 TGGCAGGTCA CAAGAAAGAC ATTGGCCGAT TGAAACTTCT CCTACCTGAC CTGCAACCCC 1441 AAAGCAACTT CTGTTTGCTC TTTGATTACC GGCTGGCCGG AGACAAAGTC GGGAAACTTC 1501 GAGTGTTTGT GAAAAACAGT AACAATGCCC TGGCATGGGA GAAGACCACG AGTGAGGATG 1561 AAAAGTGGAA GACAGGGAAA ATTCAGTTGT ATCAAGGAAC TGATGCTACC AAAAGCATCA 1621 TTTTTGAAGC AGAACGTGGC AAGGGCAAAA CCGGCGAAAT CGCAGTGGAT GGCGTCTTGC 1681 TTGTTTCAGG CTTATGTCCA GATAGCCTTT TACCTGTGGA TGACTGCCCA ACTTTCTTGT 1741 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1801 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1861 GAAAGGACGA TCCTCGACTC AAAGTGTCAT TTACACGCGT TAAGTCgaca atcaacctct 1921 ggattacaaa atttgtgaaa gatt