Construct: ORF TRCN0000476896
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009362.1_s317c1
- Derived from:
- ccsbBroadEn_10021
- DNA Barcode:
- CTGGACAGAACCAAAGTATGAAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM170A (340069)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476896
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 340069 | FAM170A | family with sequence simila... | NM_001367956.1 | 100% | 100% | |
| 2 | human | 340069 | FAM170A | family with sequence simila... | NM_182761.3 | 99.6% | 99.6% | 987_988insAGC |
| 3 | human | 340069 | FAM170A | family with sequence simila... | NM_001163991.1 | 85.4% | 85.4% | 69_70ins141;846_847insAGC |
| 4 | mouse | 225497 | Fam170a | family with sequence simila... | XM_017317888.1 | 52% | 49.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1059
- ORF length:
- 990
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaaacgacga caaaagagga aacatttgga aaatgaagag tcccaggaaa 121 ccgctgagaa gggaggagga atgtcaaagt cccaagagga tgccctgcag cctggatcca 181 ctagagtggc caaaggctgg agccaagggg tgggagaagt tacttctacc tccgaatact 241 gctcctgcgt ttcttcttca cgcaagctca tccacagtgg aatccagaga atacatcgag 301 acagccccca gcctcaatca cccctggccc aggttcagga acgaggagag actcctcccc 361 gctcacaaca tgtctccttg tcgtcctatt catcctataa gacttgtgtg tcctctctgt 421 gtgtaaacaa agaggaaagg ggcatgaaaa tatactacat gcaggtacaa atgaacaaag 481 gtgtggctgt ctcctgggag acagaggaaa ctttggagtc cttagaaaag cagccaagga 541 tggaagaagt gaccctttct gaggttgtga gggtaggtac tcccccctct gatgtgtcca 601 ccagaaacct cctgtctgac agtgagccca gtggggagga gaaagagcat gaggaaagga 661 cagaatcaga cagccTGCCA GGCTCACCCA CCGTTGAGGA CACACCCAGA GCCAAGACTC 721 CTGACTGGCT GGTGACCATG GAGAACGGCT TCAGGTGCAT GGCCTGCTGC CGGGTGTTCA 781 CCACCATGGA AGCCCTCCAG GAGCATGTGC AGTTTGGGAT CAGAGAGGGC TTCAGCTGCC 841 ACGTCTTTCA TCTCACCATG GCTCAGCTGA CAGGCAACAT GGAATCAGAG AGCACCCAAG 901 ATGAGCAGGA GGAGGAAAAT GGAAATGAGA AGGAGGAGGA AGAGAAACCA GAAGCAAAGG 961 AGGAGGAGGG GCAGCCCACA GAAGAAGACC TTGGCCTGAG GAGATCCTGG AGCCAATGTC 1021 CAGGCTGTGT GTTTCATTCT CCAAAGGACA GGAACAGCTT GCCAACTTTC TTGTACAAAG 1081 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1141 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1201 ACGACTGGAC AGAACCAAAG TATGAAGCAC GCGTTAAGTC gacaatcaac ctctggatta 1261 caaaatttgt gaaagatt