Construct: ORF TRCN0000476906
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003378.1_s317c1
- Derived from:
- ccsbBroadEn_08549
- DNA Barcode:
- GGACTTCTGGAAAGAATTGCAAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZCCHC8 (55596)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476906
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55596 | ZCCHC8 | zinc finger CCHC-type conta... | NM_017612.5 | 99.9% | 99.7% | 977C>T;1498T>C |
| 2 | human | 55596 | ZCCHC8 | zinc finger CCHC-type conta... | NM_001350935.2 | 89% | 88.8% | 501_502ins231;746C>T;1267T>C |
| 3 | human | 55596 | ZCCHC8 | zinc finger CCHC-type conta... | XM_017019606.1 | 86.1% | 85% | (many diffs) |
| 4 | human | 55596 | ZCCHC8 | zinc finger CCHC-type conta... | NM_001350936.2 | 85.9% | 85.7% | 0_1ins297;680C>T;1201T>C |
| 5 | human | 55596 | ZCCHC8 | zinc finger CCHC-type conta... | NM_001350937.2 | 66.2% | 66% | 0_1ins714;263C>T;784T>C |
| 6 | human | 55596 | ZCCHC8 | zinc finger CCHC-type conta... | NM_001350938.2 | 66.2% | 66% | 0_1ins714;263C>T;784T>C |
| 7 | human | 55596 | ZCCHC8 | zinc finger CCHC-type conta... | XR_944614.3 | 66.1% | (many diffs) | |
| 8 | human | 55596 | ZCCHC8 | zinc finger CCHC-type conta... | XM_024449044.1 | 63.4% | 63.2% | 0_1ins774;203C>T;724T>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2187
- ORF length:
- 2121
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cgcagaggtg tattttggcg atctagagct cttcgagccg ttcgaccacc 121 cagaggagtc gattccgaag cccgttcaca ctcgcttcaa ggacgacgac ggcgacgagg 181 aggacgaaaa tggggtcggc gacgcggagc tacgggagcg gcttcggcag tgcgaggaga 241 ccatcgagca gctccgcgcc gagaatcaag aacttaaacg aaaattgaac attctgactc 301 gaccgagtgg aatattggtg aacgatacta agttagatgg acctatatta cagattctat 361 tcatgaacaa tgctatttca aagcaatatc atcaagaaat agaggaattt gtatcaaatt 421 tagtaaaaag atttgaggaa cagcagaaaa atgatgtgga aaagacttcc tttaatcttt 481 tgccccagcc atccagtatt gtgctagagg aggaccacaa agtggaagag tcctgtgcca 541 ttaaaaacaa caaggaagct ttcagtgttg taggaagtgt cctgtatttt actaattttt 601 gccttgataa attggggcaa ccgcttctaa atgaaaaccc tcagctttcc gaaggatggg 661 aaatacccaa gtaccatcaa gtcttcagcc acattgtttc tctagaaggg caagaaatac 721 aagtaaaggc aaaaaggcca aagcctcact gtttcaattg tggttctgaa gaacaccaaa 781 tgaaagattg cccaatgcct cggaatgctg ctcgaataag tgaaaagaga aaagagtata 841 tggatgcctg tggagaagca aacaatcaga atttccagca gcgataccac gcagaagaag 901 tagaagaaag atttggaaga ttcaagccag gagttattag tgaggaactt caagatgcac 961 taggtgtgac agacaagagt cttccacctt ttatctatcg gatgcgccag ctagggtacc 1021 caccagggtg gctcaaagag gttgaattgg agaattcggg gcttgcactc tatgatggaa 1081 aagatggcac tgatggggaa acagaagttg gagaaataca acagaataaa agtgtcactt 1141 acgatctctc aaaattggtc aactatcctg gttttaatat atctactccc agaggaattc 1201 cagacgaatg gaggatcttt ggttccatac caatgcaggc atgtcagcag aaggatgtgt 1261 ttgccaatta ccttacttct aacttccaag cgccaggtgt gaagtctggc aacaagaggt 1321 cttcatctca ctctagccca ggtagtccaa agaagcagaa gaatgaaagc aactcagcgg 1381 gatctcccgc cgacatggag ctcgattcag atatggaggt accacatggt tctcagagca 1441 gcgaaagttt tcagtttcaa ccaccattac ctcctgacac tcctccactc ccccggggaa 1501 ctcctccacc cgtcttcacc cctccactcc caaagggcac cccgccgctg actcccagtg 1561 acccacccca gaccagaaca gcatctggag ctgtggatga ggacgcactg actctagaag 1621 aacttgaaga acagcagagg cggatctggg cagctcttga gcaggccgag agcgtaaaca 1681 gcgactccga cgttcctgtg gacacacctt taactggcaa ttccgttgcc tcatcacctt 1741 gtccaaatga gctagacctc cctgtcccgg agggaaaaac atctgaaaag cagacgctgg 1801 atgagcctga ggtaccagag atttttacaa agaaatcaga agctggacat gcctccagtc 1861 cagactcTGA GGTGACATCA CTTTGTCAGA AGGAAAAAGC AGAGTTGGCT CCGGTAAACA 1921 CTGAAGGTGC CCTTCTTGAT AATGGCAGTG TCGTACCAAA CTGTGACATC AGCAATGGGG 1981 GCAGCCAGAA GCTCTTTCCT GCAGACACCA GTCCTTCAAC GGCCACTAAA ATTCATAGCC 2041 CTATACCTGA CATGAGCAAA TTTGCAACTG GAATCACGCC ATTTGAATTT GAGAATATGG 2101 CAGAATCTAC TGGAATGTAC CTCAGGATAA GAAGCTTGTT AAAGAACTCA CCCCGAAACC 2161 AGCAGAAAAA CAAAAAGGCC TCTGAATACC CAACTTTCTT GTACAAAGTG GTTGATATCG 2221 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 2281 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGGACTTCT 2341 GGAAAGAATT GCAAATACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 2401 aagatt