Construct: ORF TRCN0000476907
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003864.1_s317c1
- Derived from:
- ccsbBroadEn_08666
- DNA Barcode:
- CTGTAACATGGATGGCACTTAAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPPL2B (56928)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476907
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56928 | SPPL2B | signal peptide peptidase li... | NM_152988.3 | 99.8% | 100% | 726T>C;735T>C |
| 2 | human | 56928 | SPPL2B | signal peptide peptidase li... | XM_011528138.2 | 95.3% | 95.4% | (many diffs) |
| 3 | human | 56928 | SPPL2B | signal peptide peptidase li... | XR_001753719.1 | 85.8% | (many diffs) | |
| 4 | human | 56928 | SPPL2B | signal peptide peptidase li... | NM_001077238.2 | 85.3% | 85% | (many diffs) |
| 5 | human | 56928 | SPPL2B | signal peptide peptidase li... | XM_011528139.2 | 77.3% | 72.3% | (many diffs) |
| 6 | human | 56928 | SPPL2B | signal peptide peptidase li... | XM_017027017.1 | 77.3% | 72.3% | (many diffs) |
| 7 | human | 56928 | SPPL2B | signal peptide peptidase li... | XM_017027018.2 | 77.1% | 72.2% | (many diffs) |
| 8 | human | 56928 | SPPL2B | signal peptide peptidase li... | XM_017027019.1 | 74.8% | 75% | (many diffs) |
| 9 | human | 56928 | SPPL2B | signal peptide peptidase li... | XR_001753722.1 | 70.7% | (many diffs) | |
| 10 | human | 56928 | SPPL2B | signal peptide peptidase li... | XM_017027016.1 | 66.9% | 43.2% | (many diffs) |
| 11 | human | 56928 | SPPL2B | signal peptide peptidase li... | XM_011528140.1 | 65.7% | 65.8% | 0_1ins606;120T>C;129T>C |
| 12 | human | 56928 | SPPL2B | signal peptide peptidase li... | XM_024451619.1 | 64.8% | 51.4% | (many diffs) |
| 13 | human | 56928 | SPPL2B | signal peptide peptidase li... | XR_001753720.2 | 49.5% | (many diffs) | |
| 14 | human | 56928 | SPPL2B | signal peptide peptidase li... | XR_001753721.1 | 48.6% | (many diffs) | |
| 15 | human | 56928 | SPPL2B | signal peptide peptidase li... | XR_002958334.1 | 48% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1845
- ORF length:
- 1776
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggcagcg gtggcggctg cgctggcgcg gcttttggcg gcctttctgc 121 tcctcgcggc ccaggtggcc tgtgagtacg gcatggtgca cgtggtctcc caggccgggg 181 gccccgaagg caaagactac tgcatcctct acaacccgca gtgggcccat cttccgcacg 241 acctcagcaa ggcatctttc ctgcagctgc gcaactggac ggcctccctg ctctgctccg 301 cagccgacct ccccgcccgt ggcttcagca accagatccc gctggtggcg cgggggaact 361 gcaccttcta tgagaaagtg aggctggccc agggcagcgg agcacgcggg ctgctcatcg 421 tcagcaggga gaggctggtc cccccggggg gtaataagac gcagtatgat gagattggca 481 ttcccgtggc cctgctcagc tacaaagaca tgctggacat cttcacgcgt ttcggccgca 541 cggtgagggc ggcgctgtat gcgcctaagg agccggtgct ggactacaac atggtcatca 601 tcttcatcat ggctgtgggc accgtcgcca tcggcggcta ctgggccggg agtcgggacg 661 tgaagaaaag gtacatgaag cacaagcgcg acgatgggcc cgagaagcag gaggacgagg 721 cggtggacgt gacgccggtg atgacctgcg tgtttgtggt gatgtgctgc tccatgctgg 781 tgctgctcta ctacttctac gacctcctcg tgtacgtggt catcgggatc ttctgcctgg 841 cctccgccac cggcctctac agctgcctgg cgccctgtgt gcggcggctg cccttcggca 901 agtgcaggat ccccaacaac agcctgccct acttccacaa gcgcccgcag gcccgtatgc 961 tgctcctggc gctcttctgc gtggccgtca gcgtggtgtg gggcgtcttc cgcaacgagg 1021 accagtgggc ctgggtcctc caggatgccc tgggcatcgc cttctgcctc tacatgctga 1081 agaccatccg tctgcccacc ttcaaggcct gcacgctgct gctgctggtg ctgttcctct 1141 acgacatctt cttcgtgttc atcacgccct tcctgaccaa gagtgggagc agcatcatgg 1201 tggaggtggc cactgggccc tcggactcag ccacccgtga gaagctgccc atggtcctga 1261 aggtgcccag gctgaactcc tcacctctgg ccctgtgtga ccggcccttc tccctcctgg 1321 gtttcggaga cattttggtg ccagggctgc tggtggccta ctgccacagg tttgacatcc 1381 aggtacagtc ctccagggta tacttcgtgg cctgcaccat cgcctatggc gttggcctcc 1441 ttgtgacatt cgtggcactg gccctgatgc agcgtggcca gcccgctctc ctctacctgg 1501 tgcccTGCAC GCTGGTGACG AGCTGCGCTG TGGCGCTCTG GCGCCGGGAG CTGGGCGTGT 1561 TCTGGACGGG CAGCGGCTTT GCGAAAGTCC TACCTCCATC TCCGTGGGCC CCAGCACCAG 1621 CCGACGGCCC GCAGCCTCCC AAAGACTCTG CCACGCCACT CTCCCCGCAG CCGCCCAGCG 1681 AAGAACCAGC CACATCCCCC TGGCCTGCTG AGCAGTCCCC AAAATCACGC ACGTCCGAGG 1741 AGATGGGGGC TGGAGCCCCC ATGCGGGAGC CTGGGAGCCC AGCTGAATCC GAGGGCCGGG 1801 ACCAGGCCCA GCCGTCCCCG GTAACCCAGC CTGGCGCCTC GGCCTTGCCA ACTTTCTTGT 1861 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1921 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1981 GAAAGGACGA CTGTAACATG GATGGCACTT AAATACGCGT TAAGTCgaca atcaacctct 2041 ggattacaaa atttgtgaaa gatt