Construct: ORF TRCN0000476914
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014994.1_s317c1
- Derived from:
- ccsbBroadEn_14448
- DNA Barcode:
- CTTGCTTTATGTCATTGCACTATC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- LCLAT1 (253558)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476914
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 253558 | LCLAT1 | lysocardiolipin acyltransfe... | NM_001002257.3 | 99.6% | 2.1% | (many diffs) |
| 2 | human | 253558 | LCLAT1 | lysocardiolipin acyltransfe... | NM_001304445.2 | 99.6% | 2.1% | (many diffs) |
| 3 | human | 253558 | LCLAT1 | lysocardiolipin acyltransfe... | XM_005264245.3 | 99.6% | 2.1% | (many diffs) |
| 4 | human | 253558 | LCLAT1 | lysocardiolipin acyltransfe... | XM_011532742.3 | 99.6% | 2.1% | (many diffs) |
| 5 | human | 253558 | LCLAT1 | lysocardiolipin acyltransfe... | XM_017003746.1 | 99.6% | 2.1% | (many diffs) |
| 6 | human | 253558 | LCLAT1 | lysocardiolipin acyltransfe... | NM_182551.5 | 90.4% | 1.9% | (many diffs) |
| 7 | human | 253558 | LCLAT1 | lysocardiolipin acyltransfe... | XM_005264244.1 | 90.4% | 1.9% | (many diffs) |
| 8 | human | 253558 | LCLAT1 | lysocardiolipin acyltransfe... | XM_011532741.2 | 90.4% | 1.9% | (many diffs) |
| 9 | human | 253558 | LCLAT1 | lysocardiolipin acyltransfe... | NM_001304446.2 | 66.9% | 1.1% | 0_1ins371;390C>A;693C>T |
| 10 | human | 253558 | LCLAT1 | lysocardiolipin acyltransfe... | XM_011532744.3 | 66.9% | 1.1% | 0_1ins371;390C>A;693C>T |
| 11 | human | 253558 | LCLAT1 | lysocardiolipin acyltransfe... | XM_017003747.2 | 50.9% | 3.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 99
- ORF length:
- 30
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtgtcatgg aaaggattta ctttatactg actctgtttt ggggaagctt 121 ttttggaagc attttcatgc tgagtccctt tttacctttg atgtttgtaa acccatcttg 181 gtatcgctgg atcaacaacc gccttgtgtc aacatggctc accctacctg tggcattatt 241 ggagaccatg tttggtgtaa aagtgattat aactggggat gcatttgttc ctggagaaag 301 aagtgtcatt atcatgaacc atcggacaag aatggactgg atgttcctgt ggaattgcct 361 gatgcgatat agctacctca gattggagaa aatttgcctc aaagcgagtc tcaaaggtgt 421 tcctggattt ggttgggcca tgcaggctgc tgcctatatc ttcattcata ggaaatggaa 481 ggatgacaag agccatttcg aagacatgat tgattacttt tgtgatattc acgaaccact 541 tcaactcctc atattcccag aagggactga tctcacagaa aacagcaagt ctcgaagtaa 601 tgcatttgct gaaaaaaatg gacttcagaa atatgaatat gttttacatc caagaactac 661 aggctttact tttgtggtag accgtctaag agaaggtaag aaccttgatg ctgtccatga 721 tatcactgtg gcgtatcctc acaacattcc tcaatcagag aagcacctcc tccaaggaga 781 ctttcccagg gaaatccact ttcacgtcca ccggtatcca atagacacac tCCCCACATC 841 CAAGGAGGAC CTTCAACTCT GGTGCCACAA ACGGTGGGAA GAGAAAGAAG AGAGGCTGCG 901 TTCCTTCTAT CAAGGGGAGA AGAATTTTTA TTTTACCGGA CAGAGTGTCA TTCCACCTTG 961 CAAGTCTGAA CTCAGGGTCC TTGTGGTCAA ATTGCTCTCT ATACTGTATT GGACCCTGTT 1021 CAGCCCTGCA ATGTGCCTAC TCATATATTT GTACAGTCTT GTTAAGTGGT ATTTTATAAT 1081 CACCATTGTA ATCTTTGTGC TGCAAGAGAG AATATTTGGT GGACTGGAGA TTATAGAACT 1141 TGCATGTTAC CGACTTTTAC ACAAACAGCC ACATTTAAAT TCAAAGAAAA ATGAGTTGCC 1201 AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT 1261 CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA 1321 TATATCTTGT GGAAAGGACG ACTTGCTTTA TGTCATTGCA CTATCACGCG TTAAGTCgac 1381 aatcaacctc tggattacaa aatttgtgaa agatt