Construct: ORF TRCN0000476940
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009817.1_s317c1
- Derived from:
- ccsbBroadEn_05057
- DNA Barcode:
- CTAAAATAGCACTTGACAGATAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WDR49 (151790)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476940
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 151790 | WDR49 | WD repeat domain 49 | NM_001366158.1 | 100% | 100% | |
| 2 | human | 151790 | WDR49 | WD repeat domain 49 | NM_001348951.2 | 67.1% | 67.1% | 1_1023del |
| 3 | human | 151790 | WDR49 | WD repeat domain 49 | NM_001348952.2 | 67.1% | 67.1% | 1_1023del |
| 4 | human | 151790 | WDR49 | WD repeat domain 49 | NM_001366157.1 | 66.4% | 66.4% | 1_1023del;1789_1821del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2157
- ORF length:
- 2091
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ttggagagag aaatcaaaaa agcgtcttaa tatgacatcc ttcaacattg 121 cccagggcat tcatgctttt gattatcact ctcggctcaa tttaattgca actgctggca 181 ttaacaataa agtttgcctt tggaatccct atgttgtctc taaaccagtg ggtgtccttt 241 ggggccactc agccagtgta atagccgtcc aattctttgt ggaaagaaaa caacttttca 301 gcttctccaa ggataaagtt ttgagactct gggatattca acaccagctg tccatccaga 361 ggatagcttg ttctttcccc aaaagtcagg acttcagatg tctcttccac tttgatgaag 421 cccatggacg acttttcatc tcgtttaata accagctagc attgttggca atgaaaagtg 481 aagccagcaa gagggtgaaa agccatgaga aagcagtcac ttgtgttctt tacaattcta 541 tcttgaagca ggtaatcagc tctgatacag ggtctactgt ttccttctgg atgatagaca 601 ctgggcagaa aatcaaacag tttactggtt gccacggcaa cgcagaaatc agcactatgg 661 cccttgatgc aaatgagact cggcttttga ctggcagcac agatgggact gtaaagatat 721 gggacttcaa tggatattgt caccatacac taaatgttgg gcaagatgga gctgtggata 781 tttcacaaat cctcattctt aagaagaaaa tactggttac aggctgggag agggctatta 841 ctgtgtttcg accccaaaac ttcaatcaat ttttcatcca gcctgaagaa tggaaaggag 901 gtatacagca ccatgatgac atcttgtgtg ctgcgttttt acctccacaa actcttgtta 961 cggggagtta tgatggagaa attgttctat ggaacaatag cacagagaat gctcaccatg 1021 ttcttcaccc tgattaccag aggttgctaa agtcaaaatt agatacaaaa cctcaaaaac 1081 ttctcagtgc tgggagaagc caaccctccc accccatggc agaccattct accacgggag 1141 tccgcaactt tgagattgac actgagggca aaaatgctgt tatgagactt tgctttctta 1201 aagcaagaaa aaacactgca gtgacaggag gagctaacct ggtatcatgt ggaggatctg 1261 gttatgtcag attttgggat atatataaga agcaacttct ggctgaattt ttggctcata 1321 gtggagttgg atcgattatt atgtctactg ataagatgaa tcgatacctt accacaggag 1381 atcttgatgg atggttgaaa atctggaata tagaggagta ctgtcttaac tccagtaaga 1441 acaaaatcac caaggcccca actctgataa gatcattcca acctcatgag gaccgaataa 1501 gttccttaga gatgtgtgag ccaggtggtc agttactgat tatctcctcc tctgcagact 1561 gcagtatttg tgtgactggt gtctgcaatg ctcctgtttg gatctttggt caggcaaagc 1621 actggcatat tgaaaactgc cttttccttc ctaaaagaga tactaattta gtggaaagtg 1681 agattcaaaa ggaaatttct ttattttcta aggaggaatc ttgtttagac ccaacagaac 1741 attctctact taataagaaa aacaaagatg actcaacata caatgtcaga ccatcagaag 1801 atataaattt AGATATAAAA TATAAGGAAA GAAGTACCTG CATGAAAGAA ACACAAAAAC 1861 CTTATTATGG TGAAGTTATA AAAAAATCAT TCAGTACATT TAGGTCATTA AACATTGGAG 1921 CCCTGGAAGA GCTGCCTGAA GTGAATAAAC CTGCTTTCCT TCTAGACCCT GAGAAATACT 1981 TTAGGAAAGA ACCAGAGGAA GAGCGTCCCC AAATTCTGGA GGCCCCGTCA CTTTTTAAAA 2041 CTTTGAAAGC TGTATTTGAT GAGAAAAACC TGTTTCCCAA GGAAATTCTG CATCATGAAC 2101 GAAAAGCCAA GCAATTATGC CAAGAAAAAA GTTGTGAAGT GAAGAAAAAT AAGAAGTACC 2161 CAACTTCCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 2221 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2281 ATATATCTTG TGGAAAGGAC GACTAAAATA GCACTTGACA GATAGCACGC GTTAAGTCga 2341 caatcaacct ctggattaca aaatttgtga aagatt