Construct: ORF TRCN0000476940

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009817.1_s317c1
Derived from:
ccsbBroadEn_05057
DNA Barcode:
CTAAAATAGCACTTGACAGATAGC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
WDR49 (151790)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000476940
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 151790 WDR49 WD repeat domain 49 NM_001366158.1 100% 100%
2 human 151790 WDR49 WD repeat domain 49 NM_001348951.2 67.1% 67.1% 1_1023del
3 human 151790 WDR49 WD repeat domain 49 NM_001348952.2 67.1% 67.1% 1_1023del
4 human 151790 WDR49 WD repeat domain 49 NM_001366157.1 66.4% 66.4% 1_1023del;1789_1821del
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2157
ORF length:
2091
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc ttggagagag aaatcaaaaa agcgtcttaa tatgacatcc ttcaacattg
121cccagggcat tcatgctttt gattatcact ctcggctcaa tttaattgca actgctggca
181ttaacaataa agtttgcctt tggaatccct atgttgtctc taaaccagtg ggtgtccttt
241ggggccactc agccagtgta atagccgtcc aattctttgt ggaaagaaaa caacttttca
301gcttctccaa ggataaagtt ttgagactct gggatattca acaccagctg tccatccaga
361ggatagcttg ttctttcccc aaaagtcagg acttcagatg tctcttccac tttgatgaag
421cccatggacg acttttcatc tcgtttaata accagctagc attgttggca atgaaaagtg
481aagccagcaa gagggtgaaa agccatgaga aagcagtcac ttgtgttctt tacaattcta
541tcttgaagca ggtaatcagc tctgatacag ggtctactgt ttccttctgg atgatagaca
601ctgggcagaa aatcaaacag tttactggtt gccacggcaa cgcagaaatc agcactatgg
661cccttgatgc aaatgagact cggcttttga ctggcagcac agatgggact gtaaagatat
721gggacttcaa tggatattgt caccatacac taaatgttgg gcaagatgga gctgtggata
781tttcacaaat cctcattctt aagaagaaaa tactggttac aggctgggag agggctatta
841ctgtgtttcg accccaaaac ttcaatcaat ttttcatcca gcctgaagaa tggaaaggag
901gtatacagca ccatgatgac atcttgtgtg ctgcgttttt acctccacaa actcttgtta
961cggggagtta tgatggagaa attgttctat ggaacaatag cacagagaat gctcaccatg
1021ttcttcaccc tgattaccag aggttgctaa agtcaaaatt agatacaaaa cctcaaaaac
1081ttctcagtgc tgggagaagc caaccctccc accccatggc agaccattct accacgggag
1141tccgcaactt tgagattgac actgagggca aaaatgctgt tatgagactt tgctttctta
1201aagcaagaaa aaacactgca gtgacaggag gagctaacct ggtatcatgt ggaggatctg
1261gttatgtcag attttgggat atatataaga agcaacttct ggctgaattt ttggctcata
1321gtggagttgg atcgattatt atgtctactg ataagatgaa tcgatacctt accacaggag
1381atcttgatgg atggttgaaa atctggaata tagaggagta ctgtcttaac tccagtaaga
1441acaaaatcac caaggcccca actctgataa gatcattcca acctcatgag gaccgaataa
1501gttccttaga gatgtgtgag ccaggtggtc agttactgat tatctcctcc tctgcagact
1561gcagtatttg tgtgactggt gtctgcaatg ctcctgtttg gatctttggt caggcaaagc
1621actggcatat tgaaaactgc cttttccttc ctaaaagaga tactaattta gtggaaagtg
1681agattcaaaa ggaaatttct ttattttcta aggaggaatc ttgtttagac ccaacagaac
1741attctctact taataagaaa aacaaagatg actcaacata caatgtcaga ccatcagaag
1801atataaattt AGATATAAAA TATAAGGAAA GAAGTACCTG CATGAAAGAA ACACAAAAAC
1861CTTATTATGG TGAAGTTATA AAAAAATCAT TCAGTACATT TAGGTCATTA AACATTGGAG
1921CCCTGGAAGA GCTGCCTGAA GTGAATAAAC CTGCTTTCCT TCTAGACCCT GAGAAATACT
1981TTAGGAAAGA ACCAGAGGAA GAGCGTCCCC AAATTCTGGA GGCCCCGTCA CTTTTTAAAA
2041CTTTGAAAGC TGTATTTGAT GAGAAAAACC TGTTTCCCAA GGAAATTCTG CATCATGAAC
2101GAAAAGCCAA GCAATTATGC CAAGAAAAAA GTTGTGAAGT GAAGAAAAAT AAGAAGTACC
2161CAACTTCCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC
2221TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT
2281ATATATCTTG TGGAAAGGAC GACTAAAATA GCACTTGACA GATAGCACGC GTTAAGTCga
2341caatcaacct ctggattaca aaatttgtga aagatt

Download FASTA (ORF) (Full)