Construct: ORF TRCN0000476949
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008504.1_s317c1
- Derived from:
- ccsbBroadEn_06948
- DNA Barcode:
- CCCATCCGAACCTCTTAAATTAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ST6GAL1 (6480)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476949
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6480 | ST6GAL1 | ST6 beta-galactoside alpha-... | NM_001353916.2 | 99.9% | 100% | 837C>T |
| 2 | human | 6480 | ST6GAL1 | ST6 beta-galactoside alpha-... | NM_003032.3 | 99.9% | 100% | 837C>T |
| 3 | human | 6480 | ST6GAL1 | ST6 beta-galactoside alpha-... | NM_173216.2 | 99.9% | 100% | 837C>T |
| 4 | human | 6480 | ST6GAL1 | ST6 beta-galactoside alpha-... | NM_173217.2 | 43% | 43.1% | 0_1ins693;144C>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1284
- ORF length:
- 1218
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat tcacaccaac ctgaagaaaa agttcagctg ctgcgtcctg gtctttcttc 121 tgtttgcagt catctgtgtg tggaaggaaa agaagaaagg gagttactat gattccttta 181 aattgcaaac caaggaattc caggtgttaa agagtctggg gaaattggcc atggggtctg 241 attcccagtc tgtatcctca agcagcaccc aggaccccca caggggccgc cagaccctcg 301 gcagtctcag aggcctagcc aaggccaaac cagaggcctc cttccaggtg tggaacaagg 361 acagctcttc caaaaacctt atccctaggc tgcaaaagat ctggaagaat tacctaagca 421 tgaacaagta caaagtgtcc tacaaggggc caggaccagg catcaagttc agtgcagagg 481 ccctgcgctg ccacctccgg gaccatgtga atgtatccat ggtagaggtc acagattttc 541 ccttcaatac ctctgaatgg gagggttatc tgcccaagga gagcattagg accaaggctg 601 ggccttgggg caggtgtgct gttgtgtcgt cagcgggatc tctgaagtcc tcccaactag 661 gcagagaaat cgatgatcat gacgcagtcc tgaggtttaa tggggcaccc acagccaact 721 tccaacaaga tgtgggcaca aaaactacca ttcgcctgat gaactctcag ttggttacca 781 cagagaagcg cttcctcaaa gacagtttgt acaatgaagg aatcctaatt gtatgggacc 841 catctgtata ccactcagat atcccaaagt ggtaccagaa tccggattat aatttcttta 901 ataactacaa gacttatcgt aagctgcacc ccaatcagcc cTTTTACATC CTCAAGCCCC 961 AGATGCCTTG GGAGCTATGG GACATTCTTC AAGAAATCTC CCCAGAAGAG ATTCAGCCAA 1021 ACCCCCCATC CTCTGGGATG CTTGGTATCA TCATCATGAT GACGCTGTGT GACCAGGTGG 1081 ATATTTATGA GTTCCTCCCA TCCAAGCGCA AGACTGACGT GTGCTACTAC TACCAGAAGT 1141 TCTTCGATAG TGCCTGCACG ATGGGTGCCT ACCACCCGCT GCTCTATGAG AAGAATTTGG 1201 TGAAGCATCT CAACCAGGGC ACAGATGAGG ACATCTACCT GCTTGGAAAA GCCACACTGC 1261 CTGGCTTCCG GACCATTCAC TGCTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1321 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1381 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC CCATCCGAAC 1441 CTCTTAAATT AAAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1501 att