Construct: ORF TRCN0000476952
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015967.1_s317c1
- Derived from:
- ccsbBroadEn_04405
- DNA Barcode:
- ACTACAGTCCAACGCATTTGATCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KISS1R (84634)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476952
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84634 | KISS1R | KISS1 receptor | NM_032551.5 | 100% | 100% | |
| 2 | human | 84634 | KISS1R | KISS1 receptor | XM_017027382.1 | 56.7% | 44% | 503_504ins233;678_679ins283 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1263
- ORF length:
- 1194
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcacaccgtg gctacgtccg gacccaacgc gtcctggggg gcaccggcca 121 acgcctccgg ctgcccgggc tgtggcgcca acgcctcgga cggcccagtc ccttcgccgc 181 gggccgtgga cgcctggctc gtgccgctct tcttcgcggc gctgatgctg ctgggcctgg 241 tggggaactc gctggtcatc tacgtcatct gccgccacaa gccgatgcgg accgtgacca 301 acttctacat cgccaacctg gcggccacgg acgtgacctt cctcctgtgc tgcgtcccct 361 tcacggccct gctgtacccg ctgcccggct gggtgctggg cgacttcatg tgcaagttcg 421 tcaactacat ccagcaggtc tcggtgcagg ccacgtgtgc cactctgacc gccatgagtg 481 tggaccgctg gtacgtgacg gtgttcccgt tgcgcgccct gcaccgccgc acgccccgcc 541 tggcgctggc tgtcagcctc agcatctggg taggctctgc ggcggtgtct gcgccggtgc 601 tcgccctgca ccgcctgtca cccgggccgc gcgcctactg cagtgaggcc ttccccagcc 661 gcgccctgga gcgcgccttc gcactgtaca acctgctggc gctgtacctg ctgccgctgc 721 tcgccacctg cgcctgctat gcggccatgc tgcgccacct gggccgggtc gccgtgcgcc 781 ccgcgcccgc cgatagcgcc ctgcaggggc aggtgctggc agagcgcgca ggcgccgtgc 841 gggccaaggt ctcgcggctg gtggcggccg tggtcctgct cttcgccgcc tgctggggcc 901 ccatCCAGCT GTTCCTGGTG CTGCAGGCGC TGGGCCCCGC GGGCTCCTGG CACCCACGCA 961 GCTACGCCGC CTACGCGCTT AAGACCTGGG CTCACTGCAT GTCCTACAGC AACTCCGCGC 1021 TGAACCCGCT GCTCTACGCC TTCCTGGGCT CGCACTTCCG ACAGGCCTTC CGCCGCGTCT 1081 GCCCCTGCGC GCCGCGCCGC CCCCGCCGCC CCCGCCGGCC CGGACCCTCG GACCCCGCAG 1141 CCCCACACGC GGAGCTGCTC CGCCTGGGGT CCCACCCGGC CCCCGCCAGG GCGCAGAAGC 1201 CAGGGAGCAG TGGGCTGGCC GCGCGCGGGC TGTGCGTCCT GGGGGAGGAC AACGCCCCTC 1261 TCTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1321 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1381 GGCTTTATAT ATCTTGTGGA AAGGACGAAC TACAGTCCAA CGCATTTGAT CAACGCGTTA 1441 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt