Construct: ORF TRCN0000476964
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000072.1_s317c1
- Derived from:
- ccsbBroadEn_14465
- DNA Barcode:
- CTACCTTGACCGTGTAACTAGACT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- ARL10 (285598)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476964
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 285598 | ARL10 | ADP ribosylation factor lik... | NM_001317948.1 | 99.5% | 11.2% | (many diffs) |
| 2 | human | 285598 | ARL10 | ADP ribosylation factor lik... | XM_011534530.3 | 78.2% | 12.9% | (many diffs) |
| 3 | human | 285598 | ARL10 | ADP ribosylation factor lik... | NM_173664.6 | 74.3% | 12.5% | (many diffs) |
| 4 | human | 285598 | ARL10 | ADP ribosylation factor lik... | XM_011534529.3 | 71% | 11.1% | (many diffs) |
| 5 | human | 285598 | ARL10 | ADP ribosylation factor lik... | XM_011534531.3 | 69.5% | 15.2% | (many diffs) |
| 6 | human | 285598 | ARL10 | ADP ribosylation factor lik... | XM_017009372.1 | 46.8% | 21.2% | (many diffs) |
| 7 | mouse | 56795 | Arl10 | ADP-ribosylation factor-lik... | XM_006517306.3 | 58.7% | 16.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 432
- ORF length:
- 366
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gccgcggccg ctgggcccct tggtgctggc gctcggcgtg cgccgcggcg 121 gngctgggct cggtgctctt catcctctgg aagacctact tcggccgcgg ccgagagcgg 181 cgctgggacc ggggagaggc ctggtggggc gcggaggctg cccgcctccc cgagtgggac 241 gagtgggacc ccgaggacga ggaggacgag gagccggcgc tggaggagct ggaacagcgc 301 gaggtgctgg tgctggggct ggatggcgca ggcaagagca cgttcctgcg cgtgttgtcg 361 gggaagccac cgctggaagg ccacatcccc acctggggct tcaactccgt gcgtctgccc 421 accaaggact ttgaggtgga cctgctagaa attgggggca gccagaacct gcgcttctac 481 tggaaggagt ttgtgagcga ggtggatgtg ctggtgtttg tggtggacTC GGCTGACCGA 541 CTGCGGCTGC CCTGGGCCCG ACAGGAGCTG CACAAGCTGC TGGACAAGGA CCCTGACCTG 601 CCTGTCGTCG TGGTGGCCAA CAAGCAGGTG AGGGCTGTGA GAGGGCAGCT CGGTCCAGGT 661 GACATCCACT CAGAGATGCT TGAGCAGGGG CAAGGAGCGC TGCCTGGGCC CATGGCCTGG 721 AGAGGGTGGC TGAGATGCTG CCCCCACCTC TTCTACCTAT GCCCTGTCCT GATCCCTGCC 781 TCTGTCTTCC TTCCTCTTTG CCTCCCTATC ATCTCGTACT CTGGAGAGAT GAGAAAAATA 841 ATTATTAAGT GCCTCCTATA TGCCAGGCAT GGTGTTTTGT TTTTGTTTTT TTTTTGCCCA 901 ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC 961 GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT 1021 ATATCTTGTG GAAAGGACGA CTACCTTGAC CGTGTAACTA GACTACGCGT TAAGTCgaca 1081 atcaacctct ggattacaaa atttgtgaaa gatt