Construct: ORF TRCN0000476980
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008772.1_s317c1
- Derived from:
- ccsbBroadEn_11001
- DNA Barcode:
- ACTACTCGCACCGTTAGCAGCTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NOTCH2 (4853)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476980
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4853 | NOTCH2 | notch receptor 2 | NM_001200001.1 | 99.9% | 99.9% | 2295A>G;2837G>A |
| 2 | human | 4853 | NOTCH2 | notch receptor 2 | NM_024408.4 | 49.7% | 49.4% | (many diffs) |
| 3 | human | 100996763 | NOTCH2NLB | notch 2 N-terminal like B | NM_001364007.2 | 21.3% | 20.3% | (many diffs) |
| 4 | human | 101929796 | NOTCH2NLR | notch 2 N-terminal like R | NR_157049.1 | 21.1% | (many diffs) | |
| 5 | human | 100996717 | NOTCH2NLC | notch 2 N-terminal like C | NM_001364013.1 | 20.7% | 18.6% | (many diffs) |
| 6 | human | 100996763 | NOTCH2NLB | notch 2 N-terminal like B | XM_006711700.4 | 20.2% | 18% | (many diffs) |
| 7 | human | 388677 | NOTCH2NLA | notch 2 N-terminal like A | NM_001364006.1 | 19.3% | 18.3% | (many diffs) |
| 8 | human | 100996763 | NOTCH2NLB | notch 2 N-terminal like B | NM_001364008.1 | 19.2% | 18.2% | (many diffs) |
| 9 | human | 100996763 | NOTCH2NLB | notch 2 N-terminal like B | XM_011510256.2 | 19.2% | 18.3% | (many diffs) |
| 10 | human | 100996763 | NOTCH2NLB | notch 2 N-terminal like B | XM_024452030.1 | 18.9% | 17.9% | (many diffs) |
| 11 | human | 100996763 | NOTCH2NLB | notch 2 N-terminal like B | XM_024452031.1 | 18.9% | 17.9% | (many diffs) |
| 12 | human | 100996763 | NOTCH2NLB | notch 2 N-terminal like B | XM_024452032.1 | 18.9% | 17.9% | (many diffs) |
| 13 | human | 100996763 | NOTCH2NLB | notch 2 N-terminal like B | XM_024452035.1 | 18.9% | 17.9% | (many diffs) |
| 14 | human | 100996717 | NOTCH2NLC | notch 2 N-terminal like C | NM_001364012.2 | 18.2% | 17.3% | (many diffs) |
| 15 | human | 388677 | NOTCH2NLA | notch 2 N-terminal like A | NM_203458.4 | 18.2% | 17.3% | (many diffs) |
| 16 | mouse | 18129 | Notch2 | notch 2 | NM_010928.2 | 43.4% | 46% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3774
- ORF length:
- 3705
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcccgccctg cgccccgctc tgctgtgggc gctgctggcg ctctggctgt 121 gctgcgcggc ccccgcgcat gcattgcagt gtcgagatgg ctatgaaccc tgtgtaaatg 181 aaggaatgtg tgttacctac cacaatggca caggatactg caaatgtcca gaaggcttct 241 tgggggaata ttgtcaacat cgagacccct gtgagaagaa ccgctgccag aatggtggga 301 cttgtgtggc ccaggccatg ctggggaaag ccacgtgccg atgtgcctca gggtttacag 361 gagaggactg ccagtactca acatctcatc catgctttgt gtctcgaccc tgcctgaatg 421 gcggcacatg ccatatgctc agccgggata cctatgagtg cacctgtcaa gtcgggttta 481 caggtaagga gtgccaatgg acggatgcct gcctgtctca tccctgtgca aatggaagta 541 cctgtaccac tgtggccaac cagttctcct gcaaatgcct cacaggcttc acagggcaga 601 aatgtgagac tgatgtcaat gagtgtgaca ttccaggaca ctgccagcat ggtggcacct 661 gcctcaacct gcctggttcc taccagtgcc agtgccctca gggcttcaca ggccagtact 721 gtgacagcct gtatgtgccc tgtgcaccct caccttgtgt caatggaggc acctgtcggc 781 agactggtga cttcactttt gagtgcaact gccttccagg ttttgaaggg agcacctgtg 841 agaggaatat tgatgactgc cctaaccaca ggtgtcagaa tggaggggtt tgtgtggatg 901 gggtcaacac ttacaactgc cgctgtcccc cacaatggac aggacagttc tgcacagagg 961 atgtggatga atgcctgctg cagcccaatg cctgtcaaaa tgggggcacc tgtgccaacc 1021 gcaatggagg ctatggctgt gtatgtgtca acggctggag tggagatgac tgcagtgaga 1081 acattgatga ttgtgccttc gcctcctgta ctccaggctc cacctgcatc gaccgtgtgg 1141 cctccttctc ttgcatgtgc ccagagggga aggcaggtct cctgtgtcat ctggatgatg 1201 catgcatcag caatccttgc cacaaggggg cactgtgtga caccaacccc ctaaatgggc 1261 aatatatttg cacctgccca caaggctaca aaggggctga ctgcacagaa gatgtggatg 1321 aatgtgccat ggccaatagc aatccttgtg agcatgcagg aaaatgtgtg aacacggatg 1381 gcgccttcca ctgtgagtgt ctgaagggtt atgcaggacc tcgttgtgag atggacatca 1441 atgagtgcca ttcagacccc tgccagaatg atgctacctg tctggataag attggaggct 1501 tcacatgtct gtgcatgcca ggtttcaaag gtgtgcattg tgaattagaa ataaatgaat 1561 gtcagagcaa cccttgtgtg aacaatgggc agtgtgtgga taaagtcaat cgtttccagt 1621 gcctgtgtcc tcctggtttc actgggccag tttgccagat tgatattgat gactgttcca 1681 gtactccgtg tctgaatggg gcaaagtgta tcgatcaccc gaatggctat gaatgccagt 1741 gtgccacagg tttcactggt gtgttgtgtg aggagaacat tgacaactgt gaccccgatc 1801 cttgccacca tggtcagtgt caggatggta ttgattccta cacctgcatc tgcaatcccg 1861 ggtacatggg cgccatctgc agtgaccaga ttgatgaatg ttacagcagc ccttgcctga 1921 acgatggtcg ctgcattgac ctggtcaatg gctaccagtg caactgccag ccaggcacgt 1981 caggggttaa ttgtgaaatt aattttgatg actgtgcaag taacccttgt atccatggaa 2041 tctgtatgga tggcattaat cgctacagtt gtgtctgctc accaggattc acagggcaga 2101 gatgtaacat tgacattgat gagtgtgcct ccaatccctg tcgcaagggt gcaacatgta 2161 tcaacggtgt gaatggtttc cgctgtatat gccccgaggg accccatcac cccagctgct 2221 actcacaggt gaacgaatgc ctgagcaatc cctgcatcca tggaaactgt actggaggtc 2281 tcagtggata taagtgtctc tgtgatgcag gctgggttgg catcaactgt gaagtggaca 2341 aaaatgaatg cctttcgaat ccgtgccaga atggaggaac ttgtgacaat ctggtgaatg 2401 gatacaggtg tacttgcaag aagggcttta aaggctataa ctgccaggtg aatattgatg 2461 aatgtgcctc aaatccatgc ctgaaccaag gaacctgctt tgatgacata agtggctaca 2521 cttgccactg tgtgctgcca tacacaggca agaattgtca gacagtattg gctccctgtt 2581 ccccaaaccc ttgtgagaat gctgctgttt gcaaagagtc accaaatttt gagagttata 2641 cttgcttgtg tgctcctggc tggcaaggtc agcggtgtac cattgacatt gacgagtgta 2701 tctccaagcc ctgcatgaac catggtctct gccataacac ccagggcagc tacatgtgtg 2761 aatgtccacc aggcttcagt ggtatggact gtgaggagga cattgatgac tgccttgcca 2821 atccttgcca gaatggaggt tcctgtatgg atggagtgaa tactttctcc tgcctctgcc 2881 ttccgggttt cactggggat aagtaccaga cagacatgaa tgagtgtctg agtgaaccct 2941 gtaagaatgg agggacctgc tctgactacg tcaacagtta cacttgcaag tgccaggcag 3001 gatttgatgg agtccattgt gagaacaaca tcaatgagtg cactgagagc tcctgtttca 3061 atggtggcac atgtgttgat gggattaact ccttctcttg cttgtgccct gtgggtttca 3121 ctggatcctt ctgcctccat gagatcaatg aatgcagctc tcatccatgc ctgaatgagg 3181 gaacgtgtgt tgatggcctg ggtacctacc gctgcagctg ccccctgggc tacactggga 3241 aaaactgtca gaccctggtg aatctctgca gtcggtctcc atgtaaaaac aaaggtactt 3301 gcgttcagaa aaaagcagag tcccagtgcc tatgtccatc tggatgggct ggtgccTATT 3361 GTGACGTGCC CAATGTCTCT TGTGACATAG CAGCCTCCAG GAGAGGTGTG CTTGTTGAAC 3421 ACTTGTGCCA GCACTCAGGT GTCTGCATCA ATGCTGGCAA CACGCATTAC TGTCAGTGCC 3481 CCCTGGGCTA TACTGGGAGC TACTGTGAGG AGCAACTCGA TGAGTGTGCG TCCAACCCCT 3541 GCCAGCACGG GGCAACATGC AGTGACTTCA TTGGTGGATA CAGATGCGAG TGTGTCCCAG 3601 GCTATCAGGG TGTCAACTGT GAGTATGAAG TGGATGAGTG CCAGAATCAG CCCTGCCAGA 3661 ATGGAGGCAC CTGTATTGAC CTTGTGAACC ATTTCAAGTG CTCTTGCCCA CCAGGCACTC 3721 GGGGTATGAA ATCATCCTTA TCCATTTTCC ATCCAGGGCA TTGTCTTAAG TTATTGCCAA 3781 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 3841 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 3901 TATCTTGTGG AAAGGACGAA CTACTCGCAC CGTTAGCAGC TCTACGCGTT AAGTCgacaa 3961 tcaacctctg gattacaaaa tttgtgaaag att