Construct: ORF TRCN0000476982
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009023.1_s317c1
- Derived from:
- ccsbBroadEn_07075
- DNA Barcode:
- GCACTGAGCGGCTTCCAGTCGAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TLR5 (7100)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476982
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7100 | TLR5 | toll like receptor 5 | NM_003268.6 | 99.9% | 100% | 981T>C;2523A>G |
| 2 | human | 7100 | TLR5 | toll like receptor 5 | XM_005273241.4 | 99.9% | 100% | 981T>C;2523A>G |
| 3 | human | 7100 | TLR5 | toll like receptor 5 | XM_005273242.4 | 99.9% | 100% | 981T>C;2523A>G |
| 4 | human | 7100 | TLR5 | toll like receptor 5 | XM_005273243.4 | 99.9% | 100% | 981T>C;2523A>G |
| 5 | human | 7100 | TLR5 | toll like receptor 5 | XM_006711504.3 | 99.9% | 100% | 981T>C;2523A>G |
| 6 | human | 7100 | TLR5 | toll like receptor 5 | XM_006711505.3 | 99.9% | 100% | 981T>C;2523A>G |
| 7 | human | 7100 | TLR5 | toll like receptor 5 | XM_006711506.3 | 99.9% | 100% | 981T>C;2523A>G |
| 8 | human | 7100 | TLR5 | toll like receptor 5 | XM_011509937.2 | 99.9% | 100% | 981T>C;2523A>G |
| 9 | human | 7100 | TLR5 | toll like receptor 5 | XM_017002208.1 | 99.9% | 100% | 981T>C;2523A>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2643
- ORF length:
- 2574
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggagaccac ctggaccttc tcctaggagt ggtgctcatg gccggtcctg 121 tgtttggaat tccttcctgc tcctttgatg gccgaatagc cttttatcgt ttctgcaacc 181 tcacccaggt cccccaggtc ctcaacacca ctgagaggct cctgctgagc ttcaactata 241 tcaggacagt cactgcttca tccttcccct ttctggaaca gctgcagctg ctggagctcg 301 ggagccagta tacccccttg actattgaca aggaggcctt cagaaacctg cccaacctta 361 gaatcttgga cctgggaagt agtaagatat acttcttgca tccagatgct tttcagggac 421 tgttccatct gtttgaactt agactgtatt tctgtggtct ctctgatgct gtattgaaag 481 atggttattt cagaaattta aaggctttaa ctcgcttgga tctatccaaa aatcagattc 541 gtagccttta ccttcatcct tcatttggga agttgaattc cttaaagtcc atagattttt 601 cctccaacca aatattcctt gtatgtgaac atgagctcga gcccctacaa gggaaaacgc 661 tctccttttt tagcctcgca gctaatagct tgtatagcag agtctcagtg gactggggaa 721 aatgtatgaa cccattcaga aacatggtgc tggagatact agatgtttct ggaaatggct 781 ggacagtgga catcacagga aactttagca atgccatcag caaaagccag gccttctctt 841 tgattcttgc ccaccacatc atgggtgccg ggtttggctt ccataacatc aaagatcctg 901 accagaacac atttgctggc ctggccagaa gttcagtgag acacctggat ctttcacatg 961 ggtttgtctt ctccctgaac tcacgagtct ttgagacact caaggatttg aaggttctga 1021 accttgccta caacaagata aataagatcg cagatgaagc attttacgga cttgacaacc 1081 tccaagttct caatttgtca tataaccttc tgggggaact ttacagttcg aatttctatg 1141 gactacctaa ggtagcctac attgatttgc aaaagaatca cattgcaata attcaagacc 1201 aaacattcaa attcctggaa aaattacaga ccttggatct ccgagacaat gctcttacaa 1261 ccattcattt tattccaagc atacccgata tcttcttgag tggcaataaa ctagtgactt 1321 tgccaaagat caaccttaca gcgaacctca tccacttatc agaaaacagg ctagaaaatc 1381 tagatattct ctactttctc ctacgggtac ctcatctcca gattctcatt ttaaatcaaa 1441 atcgcttctc ctcctgtagt ggagatcaaa ccccttcaga gaatcccagc ttagaacagc 1501 ttttccttgg agaaaatatg ttgcaacttg cctgggaaac tgagctctgt tgggatgttt 1561 ttgagggact ttctcatctt caagttctgt atttgaatca taactatctt aattcccttc 1621 caccaggagt atttagccat ctgactgcat taaggggact aagcctcaac tccaacaggc 1681 tgacagttct ttctcacaat gatttacctg ctaatttaga gatcctggac atatccagga 1741 accagctcct agctcctaat cctgatgtat ttgtatcact tagtgtcttg gatataactc 1801 ataacaagtt catttgtgaa tgtgaactta gcacttttat caattggctt aatcacacca 1861 atgtcactat agctgggcct cctgcagaca tatattgtgt gtaccctgac tcgttctctg 1921 gggtttccct cttctctctt tccacggaag gttgtgatga agaggaagtc ttaaagtccc 1981 taaagttctc ccttttcatt gtatgcactg tcactctgac tctgttcctc atgaccatcc 2041 tcacagtcac aaagttccgg ggcttctgtt ttatctgtta taagacagcc cagagactgg 2101 tgttcaagga ccatccccag ggcacagaac ctgatatgta caaatatgat gcctatttgt 2161 gcttcagcag caaagacttc acatgggtgc agaatgcttt gctcaaacac ctggacactc 2221 aatacagtga ccaaaacaga ttcaaccTGT GCTTTGAAGA AAGAGACTTT GTCCCAGGAG 2281 AAAACCGCAT TGCCAATATC CAGGATGCCA TCTGGAACAG TAGAAAGATC GTTTGTCTTG 2341 TGAGCAGACA CTTCCTTAGA GATGGCTGGT GCCTTGAAGC CTTCAGTTAT GCCCAGGGCA 2401 GGTGCTTATC TGACCTTAAC AGTGCTCTCA TCATGGTGGT GGTTGGGTCC TTGTCCCAGT 2461 ACCAGTTGAT GAAACATCAA TCCATCAGAG GCTTTGTACA GAAACAGCAG TATTTGAGGT 2521 GGCCTGAGGA TCTCCAGGAT GTTGGCTGGT TTCTTCATAA ACTCTCTCAA CAGATACTAA 2581 AGAAAGAAAA GGAAAAGAAG AAAGACAATA ACATTCCGTT GCAAACTGTA GCAACCATCT 2641 CCTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 2701 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT CGATTTCTTG 2761 GCTTTATATA TCTTGTGGAA AGGACGAGCA CTGAGCGGCT TCCAGTCGAA CACGCGTTAA 2821 GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t