Construct: ORF TRCN0000476987
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016876.1_s317c1
- Derived from:
- ccsbBroadEn_01196
- DNA Barcode:
- ATATTTTTTCCTGCATAGAGCCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SERPINB13 (5275)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476987
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5275 | SERPINB13 | serpin family B member 13 | NM_012397.4 | 100% | 100% | |
| 2 | human | 5275 | SERPINB13 | serpin family B member 13 | XM_011526029.3 | 99.7% | 99.7% | 221_222insGGT |
| 3 | human | 5275 | SERPINB13 | serpin family B member 13 | NM_001307923.1 | 97.7% | 97.7% | 225_251del |
| 4 | human | 5275 | SERPINB13 | serpin family B member 13 | NM_001348267.2 | 62.7% | 58.8% | (many diffs) |
| 5 | human | 5275 | SERPINB13 | serpin family B member 13 | NM_001348268.2 | 62.7% | 58.8% | (many diffs) |
| 6 | human | 5275 | SERPINB13 | serpin family B member 13 | NM_001348269.2 | 54.2% | 54.2% | 0_1ins537 |
| 7 | human | 5275 | SERPINB13 | serpin family B member 13 | NM_001348270.2 | 54.2% | 54.2% | 0_1ins537 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1242
- ORF length:
- 1173
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggattcactt ggcgccgtca gcactcgact tgggtttgat cttttcaaag 121 agctgaagaa aacaaatgat ggcaacatct tcttttcccc tgtgggcatc ttgactgcaa 181 ttggcatggt cctcctgggg acccgaggag ccaccgcttc ccagttggag gaggtgtttc 241 actctgaaaa agagacgaag agctcaagaa taaaggctga agaaaaagag gtgattgaga 301 acacagaagc agtacatcaa caattccaaa agtttttgac tgaaataagc aaactcacta 361 atgattatga actgaacata accaacaggc tgtttggaga aaaaacatac ctcttccttc 421 aaaaatactt agattatgtt gaaaaatatt atcatgcatc tctggaacct gttgattttg 481 taaatgcagc cgatgaaagt cgaaagaaga ttaattcctg ggttgaaagc aaaacaaatg 541 aaaaaatcaa ggacttgttc ccagatggct ctattagtag ctctaccaag ctggtgctgg 601 tgaacatggt ttattttaaa gggcaatggg acagggagtt taagaaagaa aatactaagg 661 aagagaaatt ttggatgaat aagagcacaa gtaaatctgt acagatgatg acacagagcc 721 attcctttag cttcactttc ctggaggact tgcaggccaa aattctaggg attccatata 781 aaaacaacga cctaagcatg tttgtgcttc tgcccaacga catcgatggc ctggagaaga 841 taatagataa aataagtcct gagaaattgg tagagtggac tagtccaggg catatggaag 901 aaagaaaggt gaatctgcac ttgccccggt tTGAGGTGGA GGACGGTTAC GATCTAGAGG 961 CGGTCCTGGC TGCCATGGGG ATGGGCGATG CCTTCAGTGA GCACAAAGCC GACTACTCGG 1021 GAATGTCGTC AGGCTCCGGG TTGTACGCCC AGAAGTTCCT GCACAGTTCC TTTGTGGCAG 1081 TAACTGAGGA AGGCACCGAG GCTGCAGCTG CCACCGGCAT AGGCTTTACT GTCACATCCG 1141 CCCCAGGTCA TGAAAATGTT CACTGCAATC ATCCCTTCCT GTTCTTCATC AGGCACAATG 1201 AATCCAACAG CATCCTCTTC TTCGGCAGAT TTTCTTCTCC TTTGCCAACT TTCTTGTACA 1261 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1321 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1381 AGGACGAATA TTTTTTCCTG CATAGAGCCC AACGCGTTAA GTCgacaatc aacctctgga 1441 ttacaaaatt tgtgaaagat t