Construct: ORF TRCN0000476988
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017681.1_s317c1
- Derived from:
- ccsbBroadEn_13210
- DNA Barcode:
- CCGTTTACTGCCCTCCGTCTTCAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STK35 (140901)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476988
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 140901 | STK35 | serine/threonine kinase 35 | NM_080836.4 | 75% | 75% | 1_399del |
| 2 | human | 140901 | STK35 | serine/threonine kinase 35 | XM_011529174.3 | 30.6% | 27.5% | (many diffs) |
| 3 | mouse | 67333 | Stk35 | serine/threonine kinase 35 | NM_183262.3 | 68.7% | 70.6% | (many diffs) |
| 4 | mouse | 67333 | Stk35 | serine/threonine kinase 35 | NM_001038635.2 | 29.9% | 26.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1272
- ORF length:
- 1203
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaaacgggg aaggacggcg cccgcagagg tacacaaagc ccggagcgga 121 aaaggcgaag cccagtgccg cgggcgccca gcacgaagct gaggccggcg gcggcggccc 181 gggccatgga tccggtggcg gccgaggccc cgggcgaggc cttcctggcg cggcgacggc 241 ctgagggcgg tggcgggtcc gcgcggccgc gttacagcct gttggcggag atcgggcgcg 301 gcagctacgg cgtggtttat gaggcagtgg ccgggcgcag cggggcccgg gtggcggtca 361 agaagatccg ctgcgacgcc cccgagaacg tggagctggc gctggctgaa ttctgggccc 421 tcaccagcct caagcggcgc caccagaacg tcgtgcagtt tgaggagtgc gtcctgcagc 481 gcaatgggtt agcccagcgc atgagtcacg gcaacaagag ctcgcagctt tacctgcgcc 541 tggtggagac ctcgctgaaa ggagaaagga tcctgggtta tgctgaggag ccctgctatc 601 tctggtttgt catggagttc tgtgaaggtg gagacctgaa tcagtatgtc ctgtcccgga 661 ggccagaccc agccaccaac aaaagtttca tgctacagct gacgagcgcc attgccttcc 721 tgcacaaaaa ccatattgtg cacagggacc tgaagccaga caacatcctc atcacagagc 781 ggtctggcac ccccatcctc aaagtggccg actttggact aagcaaggtc tgtgctgggc 841 tggcaccccg aggcaaagag ggcaatcaag acaacaaaaa tgtgaatgtg aataagtact 901 ggctgtcctc agccTGCGGT TCGGACTTCT ACATGGCTCC TGAAGTCTGG GAGGGACACT 961 ACACAGCCAA GGCGGACATC TTTGCCCTGG GCATTATCAT CTGGGCAATG ATAGAAAGAA 1021 TCACTTTTAT TGACTCTGAG ACCAAGAAGG AGCTCCTGGG GACCTACATT AAACAGGGGA 1081 CTGAGATCGT CCCTGTTGGT GAGGCGCTGC TAGAAAACCC AAAGATGGAG TTGCACATCC 1141 CCCAAAAACG CAGGACTTCC ATGTCTGAGG GGATCAAGCA GCTCTTGAAA GATATGTTAG 1201 CTGCTAACCC ACAGGACCGG CCTGATGCCT TTGAACTTGA AACCAGAATG GACCAGGTCA 1261 CATGTGCTGC TTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1321 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1381 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACCG TTTACTGCCC TCCGTCTTCA 1441 TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t