Construct: ORF TRCN0000477004
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014264.1_s317c1
- Derived from:
- ccsbBroadEn_13229
- DNA Barcode:
- ACAAACGTTAACATACGGCACACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LOC145757 (145757)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477004
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 105369201 | LOC105369201 | uncharacterized LOC105369201 | NR_160707.1 | 21.6% | 0_1ins208;49A>G;264_1001del | |
| 2 | human | 105369201 | LOC105369201 | uncharacterized LOC105369201 | NR_160708.1 | 17.5% | 0_1ins208;49A>G;264_1282del | |
| 3 | human | 105369201 | LOC105369201 | uncharacterized LOC105369201 | NR_160709.1 | 9.9% | 0_1ins208;49A>G;264_2429del | |
| 4 | human | 105369201 | LOC105369201 | uncharacterized LOC105369201 | NR_160706.1 | 7% | 0_1ins208;49A>G;263_3516delinsG | |
| 5 | human | 105369201 | LOC105369201 | uncharacterized LOC105369201 | NR_160705.1 | 6.8% | 0_1ins208;49A>G;264_3638del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 540
- ORF length:
- 471
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagctgccc cagagtcgat tttcttggaa gcaacgcaaa gcacagcttc 121 ctgggagaag acacaatgcc tcctattgtc tctgccactt ccccTCCAGC CTCCCAGCAT 181 TTCTGCGTCT CCTCGGGAGC CACTGCCCTG TGCAAGAGCT GTTTGCTGAT GCCCAGCCGC 241 GGCTTCCAAA ACTCCCGCGA GAAGTCGTTT CTGTCCACAG TCATTGTTTC CAGACCAGCC 301 GAGCCAACTG GGGAGGAGAG GCCAGGCCCA GGGAGAGCAG TAAGAGGCCC AGCTTGCAAC 361 ACAGGCCCCC GGCGGCTGGA GTCGGCTCAT GGGTTGCTGG GGGGCAGCTG GTGCCAGCCC 421 TGCTAGACAT GTCCAGGGTC GGGGCAGAGG CCAGGGTGGC TCTCATCCAC AACGAGGCTC 481 CAGGAAGAGG CTCCAGAGGG CCCAGGTCCC TGGAGGAAGA CAGAGGGTGG ATTATCCAGT 541 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 601 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 661 TTTATATATC TTGTGGAAAG GACGAACAAA CGTTAACATA CGGCACACTA CGCGTTAAGT 721 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt