Construct: ORF TRCN0000477012
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011303.1_s317c1
- Derived from:
- ccsbBroadEn_09632
- DNA Barcode:
- CCAGTATTGGACAGCTTTCAGGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RTN4RL1 (146760)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477012
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 146760 | RTN4RL1 | reticulon 4 receptor like 1 | NM_178568.4 | 99.8% | 100% | 648G>A;759C>T |
| 2 | mouse | 237847 | Rtn4rl1 | reticulon 4 receptor-like 1 | NM_177708.5 | 85.6% | 87.8% | (many diffs) |
| 3 | mouse | 237847 | Rtn4rl1 | reticulon 4 receptor-like 1 | XM_006533225.2 | 76.3% | 77.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1392
- ORF length:
- 1323
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcttcgcaaa gggtgctgtg tggagttgct gctgctgttg gtagctgcgg 121 agctgcccct gggtggtggc tgcccacggg actgtgtgtg ctacccggcg cccatgacgg 181 tcagctgcca ggcgcacaac tttgcagcca tcccggaggg catccccgtg gacagcgagc 241 gcgtcttcct gcagaacaac cgcatcggcc tcctccagcc cggccacttc agccccgcca 301 tggtcaccct gtggatctac tcgaacaaca tcacctacat ccaccccagc accttcgagg 361 gcttcgtgca cctggaggag ctggacctcg gcgacaaccg gcagctgcgg acgctggcac 421 ccgagacctt ccagggcctg gtgaagcttc acgccctcta cctctacaag tgtgggctca 481 gcgccttgcc ggccggcgtc tttggcggcc tgcacagcct gcagtacctc tacctgcagg 541 acaaccacat cgagtacctc caggacgaca tcttcgtgga cctggtcaac ctcagccacc 601 tgtttctcca cggcaacaag ctgtggagtc tgggcccggg caccttccgg ggcctggtga 661 acctggaccg tcttttgctg cacgagaacc agctgcagtg ggtccaccac aaggcattcc 721 acgacctccg caggctgacc accctcttcc tcttcaacaa cagcctctcg gagctgcagg 781 gtgagtgcct ggccccgctg ggggccctgg agttcctccg cctcaatggc aacccctggg 841 actgtggttg tcgcgcgcgc tccctgtggg aatggctgca gaggttccgg ggctccagct 901 ccgctgtccc ctgtgtgtcc cctgggctgc ggcacggcca ggacctgaag ctgctgaggg 961 ccgaggactt ccggaactgc acgggaccAG CGTCCCCGCA CCAGATCAAG TCACACACGC 1021 TCACCACCAC CGACAGGGCC GCCCGCAAGG AACACCACTC ACCCCACGGC CCCACCAGGA 1081 GCAAGGGCCA CCCGCACGGC CCCCGGCCCG GCCACAGGAA GCCGGGGAAG AACTGCACCA 1141 ACCCCAGGAA CCGCAATCAG ATCTCTAAGG CGGGCGCCGG GAAACAGGCC CCCGAGCTGC 1201 CAGACTATGC CCCAGACTAC CAGCACAAGT TCAGTTTTGA CATCATGCCT ACGGCCCGGC 1261 CCAAGAGGAA GGGCAAGTGT GCCCGCAGGA CCCCCATCCG TGCCCCCAGC GGGGTGCAGC 1321 AGGCCTCCTC GGCCAGTTCC CTGGGGGCCT CCCTCCTGGC CTGGACACTG GGGCTGGCGG 1381 TCACTCTCCG CTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1441 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1501 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACCA GTATTGGACA GCTTTCAGGA 1561 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t