Construct: ORF TRCN0000477018
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007176.1_s317c1
- Derived from:
- ccsbBroadEn_08934
- DNA Barcode:
- AGATCCGCAGCTCATCGGATAACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHPF (79586)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477018
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79586 | CHPF | chondroitin polymerizing fa... | NM_024536.6 | 99.8% | 99.8% | (many diffs) |
| 2 | human | 79586 | CHPF | chondroitin polymerizing fa... | NM_001195731.1 | 79% | 79% | 0_1ins486;18A>G |
| 3 | human | 79586 | CHPF | chondroitin polymerizing fa... | XM_011511838.3 | 62.2% | 61.6% | (many diffs) |
| 4 | mouse | 74241 | Chpf | chondroitin polymerizing fa... | NM_001001566.3 | 86.9% | 94.3% | (many diffs) |
| 5 | mouse | 74241 | Chpf | chondroitin polymerizing fa... | NM_001001565.2 | 68.4% | 74.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2394
- ORF length:
- 2325
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcgggcatcg ctgctgctgt cggtgctgcg gcccgcaggg cccgtggccg 121 tgggcatctc cctgggcttc accctgagcc tgctcagcgt cacctgggtg gaggagccgt 181 gcggcccagg cccgccccaa cctggagact ctgagctgcc gccgcgcggc aacaccaacg 241 cggcgcgccg gcccaactcg gtgcagcccg gagcggagcg cgagaagccc ggggccggcg 301 aaggcgccgg ggagaattgg gagccgcgcg tcttgcccta ccaccctgca cagcccggcc 361 aggccgccaa aaaggccgtc aggacccgct acatcagcac ggagctgggc atcaggcaga 421 ggctgctggt ggcggtgctt acctctcaga ccacgctgcc cacgctgggc gtggccgtga 481 accgcacgct ggggcaccgg ctggagcgtg tggtgttcct gacgggcgca cggggccgcc 541 gggccccacc tggcatggca gtggtgacgc tgggcgagga gcgacccatt ggacacctgc 601 acctggcgct gcgccacctg ctggagcagc acggcgacga ctttgactgg ttcttcctgg 661 tgcctgacac cacctacacc gaggcgcacg gcctggcacg cctaactggc cacctcagcc 721 tggcctccgc cgcccacctg tacctgggcc ggccccagga cttcatcggc ggagagccca 781 cccccggccg ctactgccac ggaggctttg gggtgctgct gtcgcgcatg ctgctgcaac 841 aactgcgccc ccacctggaa ggctgccgca acgacatcgt cagtgcgcgc cctgacgagt 901 ggctgggtcg ctgcattctc gatgccaccg gggtgggctg cactggtgac cacgaggggg 961 tgcactatag ccatctggag ctgagccctg gggagccagt gcaggagggg gaccctcatt 1021 tccgaagtgc cctgacagcc caccctgtgc gtgaccctgt gcacatgtac cagctgcaca 1081 aagctttcgc ccgagctgaa ctggaacgca cgtaccagga gatccaggag ttacagtggg 1141 agatccagaa taccagccat ctggccgttg atggggaccg ggcagctgct tggcccgtgg 1201 gtattccagc accatcccgc ccggcctccc gctttgaggt gctgcgctgg gactacttca 1261 cggagcagca cgctttctcc tgcgccgatg gctcaccccg ctgcccactg cgtggggctg 1321 accgggctga tgtggccgat gttctgggga cagctctaga ggagctgaac cgccgctacc 1381 acccggcctt gcggctccag aagcagcagc tggtgaatgg ctaccgacgc tttgatccgg 1441 cccggggtat ggaatacacg ctggacttgc agctggaggc actgaccccc cagggaggcc 1501 gccggcccct cactcgccga gtgcagctgc tccggccgct gagccgcgtg gagatcttgc 1561 ctgtgcccta tgtcactgag gcctcacgtc tcactgtgct gctgcctcta gctgcggctg 1621 agcgtgacct ggcccctggc ttcttggagg cctttgccac tgcagcactg gagcctggtg 1681 atgctgcggc agccctgacc ctgctgctac tgtatgagcc gcgccaggcc cagcgcgtgg 1741 cccatgcaga tgtcttcgca cctgtcaagg cccacgtggc agagctggag cggcgtttcc 1801 ccggtgcccg ggtgccatgg ctcagtgtgc agacagccgc accctcacca ctgcgcctca 1861 tggatctact ctccaagaag cacccgctgg acacactgtt cctgctggcc gggccagaca 1921 cggtgctcac gcctgacttc ctgaaccgct gccgcatgca tgccatctcc ggctggcagg 1981 ccttctttcc catgcatttc caagccttcc acccagctgt ggccccacca caagggcctg 2041 ggcccccaga gctgggccgt gacactggcc gctttgatcg ccaggcagcc agcgaggcct 2101 gcttctacaa ctccgactac gtggcagccc gtgggcgcct ggcggcagcc tcagaacaag 2161 aagaggagct gctggagagc ctGGATGTGT ACGAGCTGTT CCTCCACTTC TCCAGTCTGC 2221 ATGTGCTGCG GGCGGTGGAG CCGGCGCTGC TGCAGCGCTA CCGGGCCCAG ACGTGCAGCG 2281 CGAGGCTCAG TGAGGACCTG TACCACCGCT GCCTCCAGAG CGTGCTTGAG GGCCTCGGCT 2341 CCCGAACCCA GCTGGCCATG CTACTCTTTG AACAGGAGCA GGGCAACAGC ACCTTGCCAA 2401 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 2461 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 2521 TATCTTGTGG AAAGGACGAA GATCCGCAGC TCATCGGATA ACAACGCGTT AAGTCgacaa 2581 tcaacctctg gattacaaaa tttgtgaaag att