Construct: ORF TRCN0000477031
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016644.1_s317c1
- Derived from:
- ccsbBroadEn_10169
- DNA Barcode:
- TATTGCCAAGGCAATTTACTTTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TSPAN11 (441631)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477031
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 441631 | TSPAN11 | tetraspanin 11 | NM_001080509.2 | 99.7% | 99.6% | 468T>C;569T>C |
| 2 | human | 441631 | TSPAN11 | tetraspanin 11 | NM_001370302.1 | 99.7% | 99.6% | 468T>C;569T>C |
| 3 | human | 441631 | TSPAN11 | tetraspanin 11 | NM_001370301.1 | 92% | 83.6% | (many diffs) |
| 4 | human | 441631 | TSPAN11 | tetraspanin 11 | XM_011520679.2 | 81.9% | 78.9% | (many diffs) |
| 5 | human | 441631 | TSPAN11 | tetraspanin 11 | XR_001748710.1 | 24.1% | (many diffs) | |
| 6 | human | 441631 | TSPAN11 | tetraspanin 11 | XR_001748709.1 | 23.7% | (many diffs) | |
| 7 | human | 441631 | TSPAN11 | tetraspanin 11 | XR_001748711.1 | 12.7% | (many diffs) | |
| 8 | mouse | 68498 | Tspan11 | tetraspanin 11 | NM_026743.3 | 84.1% | 87.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 828
- ORF length:
- 759
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcccactat aagactgagc aggacgactg gctgatcatc tacttgaagt 121 atttactctt tgtcttcaac ttcttcttct gggtcggggg agcagccgtc ctggctgtgg 181 gcatctggac cctggtggag aagagtggct acctcagcgt cctggcctcc agcacctttg 241 ccgcctccgc ctacatcctc atctttgcgg gcgtacttgt catggtgacc ggcttcctgg 301 gcttcggtgc catcctctgg gagcggaagg gctgcctctc cacgtatttc tgcctgttgc 361 tcgtcatctt cctggttgag ctggtggcgg gagtcctggc ccatgtgtat taccagaggc 421 tgagtgatga actgaagcag cacttgaacc ggactctggc tgagaactac gggcagcccg 481 gagccacgca gatcaccgcc TCAGTGGACC GACTCCAGCA GGATTTCAAG TGCTGCGGAA 541 GCAACAGCTC AGCCGACTGG CAGCACAGCA CGTACATCCT GTTGCGGGAG GCCGAGGGCC 601 GCCAGGTGCC CGACAGCTGC TGCAAGACAG TGGTGGCGCG CTGCGGCCAG CGGGCCCACC 661 CCTCCAACAT CTATAAGGTG GAGGGAGGCT GCCTCACCAA GCTGGAGCAG TTCCTGGCCG 721 ACCACCTGCT GCTTATGGGG GCAGTGGGCA TCGGGGTGGC CTGCCTGCAG ATCTGCGGGA 781 TGGTTCTCAC CTGCTGCTTG CACCAGAGGC TCCAGCGGCA TTTTTACTTG CCAACTTTCT 841 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 901 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 961 GTGGAAAGGA CGATATTGCC AAGGCAATTT ACTTTAAACG CGTTAAGTCg acaatcaacc 1021 tctggattac aaaatttgtg aaagatt