Construct: ORF TRCN0000477048
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005050.1_s317c1
- Derived from:
- ccsbBroadEn_03822
- DNA Barcode:
- CTCTTTCTGGGGTGAGCAGCATGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GATAD2B (57459)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477048
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57459 | GATAD2B | GATA zinc finger domain con... | NM_020699.4 | 100% | 100% | |
| 2 | human | 57459 | GATAD2B | GATA zinc finger domain con... | XM_005245364.4 | 100% | 100% | |
| 3 | human | 57459 | GATAD2B | GATA zinc finger domain con... | XM_024448621.1 | 100% | 100% | |
| 4 | mouse | 229542 | Gatad2b | GATA zinc finger domain con... | XM_006501359.3 | 92.8% | 98.3% | (many diffs) |
| 5 | mouse | 229542 | Gatad2b | GATA zinc finger domain con... | XM_006501361.2 | 92.8% | 98.3% | (many diffs) |
| 6 | mouse | 229542 | Gatad2b | GATA zinc finger domain con... | NM_139304.1 | 92.8% | 98.3% | (many diffs) |
| 7 | mouse | 229542 | Gatad2b | GATA zinc finger domain con... | XM_006501360.3 | 92.7% | 98.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1845
- ORF length:
- 1779
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tagaatgaca gaagatgctc ttcgcttgaa tctgttgaag cggagcttgg 121 acccagcaga tgagcgagat gatgtcctgg caaagcgact caaaatggag gggcatgagg 181 ccatggaacg tctgaaaatg ttggcattgc tcaaaaggaa ggatttggca aatcttgagg 241 tgccacatga gttacccacc aaacaggatg gcagtggtgt caagggctat gaagaaaaac 301 ttaacgggaa tctcaggcct catggagaca acaggactgc tggaaggcca ggcaaagaaa 361 acatcaatga tgagcctgtg gatatgagtg ctagacggag tgagccagag cgaggaaggc 421 taactccctc accagacatc attgttttgt ctgacaatga ggcttccagt ccccgttcca 481 gttccagaat ggaagaaaga ctcaaagcag ccaacttaga gatgtttaag gggaaaggca 541 ttgaggagcg gcagcagctt atcaagcagc tgagggatga gctacgattg gaagaagccc 601 gactggtcct gttaaagaaa ctgagacaga gtcagctaca gaaagagaat gtggtccaga 661 agactccagt tgtacagaat gcagcatcta ttgttcagcc atctcctgcc catgtgggac 721 agcagggcct atctaagctt ccctctcggc ctggggccca aggggttgaa cctcaaaatt 781 tgagaacatt acagggtcac agtgtcatcc gttcagctac caataccacc cttccacaca 841 tgttgatgtc tcaacgtgtt attgcaccaa acccagccca gctacagggt cagcggggcc 901 cgcctaagcc tggccttgta cgcaccacaa cacccaacat gaatcccgcc atcaattatc 961 aaccgcagtc aagttcttct gttccatgtc agcgtacaac atcctctgcc atctatatga 1021 accttgcttc tcatatccag ccagggacgg tgaacagagt gtcctcgcca cttcctagcc 1081 ccagcgccat gactgatgct gccaactcac aggctgcagc caaattggct cttcgcaaac 1141 agctggaaaa gacactcctg gagatcccac cccctaaacc tcctgctccc ttacttcatt 1201 tcttgcctag tgcagccaat agcgagttca tctacatggt aggcttggaa gaagtcgtac 1261 agagtgtcat tgacagccaa ggcaaaagct gtgcctcact tctgcgggtt gaaccctttg 1321 tatgtgccca gtgccgcaca gatttcaccc ctcactggaa gcaagaaaag aatggTAAGA 1381 TTCTATGTGA GCAGTGTATG ACCTCCAACC AGAAAAAGGC TCTAAAAGCT GAACACACCA 1441 ACCGGCTGAA AAATGCATTT GTGAAAGCCC TACAGCAGGA ACAGGAAATT GAACAGCGAT 1501 TACAGCAGCA GGCAGCCCTC TCCCCCACTA CGGCTCCAGC TGTGTCCAGT GTCAGTAAAC 1561 AAGAGACCAT CATGAGACAT CATACGCTTC GGCAGGCTCC ACAGCCCCAG AGCAGCCTCC 1621 AGCGTGGCAT ACCCACATCT GCCCGCTCCA TGCTTTCAAA CTTTGCACAG GCACCCCAGT 1681 TGTCTGTGCC AGGTGGCCTC CTTGGTATGC CAGGTGTCAA CATTGCATAC TTGAATACTG 1741 GCATCGGAGG ACACAAAGGC CCCAGTTTGG CAGACCGACA GCGTGAATAC CTTTTAGACA 1801 TGATCCCTCC CCGGTCTATA TCGCAGTCCA TCAGTGGACA GAAATACCCA ACTTTCTTGT 1861 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1921 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1981 GAAAGGACGA CTCTTTCTGG GGTGAGCAGC ATGTACGCGT TAAGTCgaca atcaacctct 2041 ggattacaaa atttgtgaaa gatt