Construct: ORF TRCN0000477080
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003752.1_s317c1
- Derived from:
- ccsbBroadEn_03227
- DNA Barcode:
- GGCTCATTTAAAATACTGGTCCAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DYNC1LI1 (51143)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477080
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51143 | DYNC1LI1 | dynein cytoplasmic 1 light ... | NM_016141.4 | 100% | 100% | |
| 2 | human | 51143 | DYNC1LI1 | dynein cytoplasmic 1 light ... | NM_001329135.1 | 77.8% | 77.6% | 220_221ins348 |
| 3 | mouse | 235661 | Dync1li1 | dynein cytoplasmic 1 light ... | NM_146229.2 | 86.9% | 94.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1638
- ORF length:
- 1569
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggccgtg gggcgagtcg gctccttcgg ttcttctccg ccgggattat 121 cctcgactta cactggcggc cccttgggca acgagatagc gtcgggcaac ggtggcgccg 181 cggcaggcga cgacgaggac gggcagaacc tttggtcctg catcctcagc gaggtctcca 241 cccgctcgcg ctccaagctc cctgcgggga agaacgtgct actgctgggt gaagatggag 301 ctggaaaaac aagcttaata agaaaaattc agggaataga ggagtataag aaaggaagag 361 gattggaata tttgtactta aatgtgcatg atgaagacag ggatgatcaa acaagatgta 421 atgtttggat cttagatgga gacctatatc acaaaggcct ccttaaattt tcactggatg 481 ccgtatctct gaaggatact ctagttatgc tggttgttga catgtcaaag ccttggactg 541 ctttggattc tttacagaaa tgggcaagtg ttgttagaga acatgttgac aaactgaaaa 601 tccctcctga agaaatgaaa caaatggaac aaaagttgat tagagacttc caagaatatg 661 tagagccagg agaagacttc ccggcttctc cccagagaag aaatactgcg tcacaagaag 721 acaaagatga cagtgtagtt ttacctctgg gtgcggatac acttacacat aacttgggca 781 ttccagtact agtagtttgc acaaagtgtg atgccattag tgtattggag aaagaacatg 841 actacagaga tgaacatttt gattttattc agtcacatat ccggaagttt tgtttacagt 901 atggtgcagc acttatttac acttcagtaa aagaaaacaa aaatatagac ttagtatata 961 aatacatcgt tcagaaacta tatggatttc cctataagat tcctgctgtt gttgtggaaa 1021 aggatgcagt atttattcca gcagggtggg ataatgataa gaaaatagga atattacatg 1081 aaaattttca aacattaaaa gcagaagata attttgaaga catcataact aaaccacctg 1141 ttcgaaagtt tgtacatgag aaggaaatta tggcagaaga tgatcaggtg tttcttatga 1201 agctacagtc ccttttagca aagcaaCCAC CAACTGCAGC TGGAAGGCCT GTGGATGCCT 1261 CACCAAGAGT CCCAGGAGGC TCCCCACGAA CACCAAATAG ATCTGTATCA TCTAATGTTG 1321 CCAGCGTGTC ACCCATTCCT GCTGGGTCAA AAAAAATTGA TCCAAACATG AAAGCTGGAG 1381 CTACAAGTGA AGGCGTTCTG GCAAATTTCT TCAACAGTTT GTTGAGTAAA AAGACTGGCT 1441 CTCCAGGAGG CCCTGGTGTG AGTGGTGGTA GCCCTGCAGG TGGGGCTGGA GGTGGAAGCA 1501 GTGGTTTACC ACCATCCACC AAAAAGTCAG GCCAGAAGCC TGTCTTAGAT GTTCATGCAG 1561 AACTAGACAG AATTACACGA AAACCAGTTA CAGTTTCTCC CACAACACCT ACATCTCCTA 1621 CGGAAGGAGA AGCTTCTTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1681 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1741 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGGCTCAT TTAAAATACT 1801 GGTCCAGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt