Construct: ORF TRCN0000477093
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009039.1_s317c1
- Derived from:
- ccsbBroadEn_12146
- DNA Barcode:
- AAAGCAGGGGAGCTGCTTGTAGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZWILCH (55055)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477093
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55055 | ZWILCH | zwilch kinetochore protein | NM_001287821.2 | 100% | 100% | |
| 2 | human | 55055 | ZWILCH | zwilch kinetochore protein | NM_001287822.1 | 100% | 100% | |
| 3 | human | 55055 | ZWILCH | zwilch kinetochore protein | NM_001287823.2 | 100% | 100% | |
| 4 | human | 55055 | ZWILCH | zwilch kinetochore protein | NM_017975.5 | 80.7% | 80.7% | 1_342del |
| 5 | human | 55055 | ZWILCH | zwilch kinetochore protein | XR_001751344.2 | 44.1% | 1_550del;1982_3243del | |
| 6 | human | 55055 | ZWILCH | zwilch kinetochore protein | XR_001751345.2 | 42.8% | 1_650del;2082_3343del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1500
- ORF length:
- 1431
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctcacaat cctaatatga cccatttgaa gattaatctg ccagttactg 121 cccttcctcc cctttgggta agatgtgaca gttcagatcc tgaaggtact tgttggctag 181 gagctgagct tatcacaaca aacaacagca ttacaggaat tgtcttatat gtggtcagtt 241 gtaaagctga taaaaattat tctgtaaatc ttgaaaacct aaaaaattta cacaagaaaa 301 gacatcactt gtctactgta acatccaaag gctttgccca gtatgagctc tttaagtcct 361 ctgccttgga tgatacaatc acagcatcac aaactgcgat cgctttggat atttcctgga 421 gtcctgtgga tgagattctt caaatccctc cactctcttc aactgcaact ctgaatatta 481 aagtggaatc aggagagccc agaggtcctt tgaatcatct ctacagagaa ctgaaatttc 541 ttcttgtttt ggctgatggt ttgaggactg gtgtcactga atggctcgag cccctggaag 601 caaaatctgc tgttgaactt gttcaggaat ttctgaatga cttaaataag ctggatggat 661 ttggtgattc tacaaaaaaa gacactgagg ttgagacctt gaagcatgac actgctgcag 721 tcgatcgttc cgtcaagcgt cttttcaaag ttcggagtga tcttgatttt gctgagcaac 781 tgtggtgcaa aatgagcagt agtgtgattt cataccaaga cttggtgaag tgtttcacat 841 tgatcatcca gagtctacaa cgtggtgata tacagccatg gctccatagt ggaagtaaca 901 gtttactaag taagctcatt catcagtctt atcatggaac catggacaca gtttctctca 961 gtgggactat tccagttcaa atgcttttgg aaattggttt ggacaaacta aagaaagatt 1021 atatcagttt tttcataggt caggaacttg catctttgaa tcatttggaa tacttcattg 1081 ctccatcagt agatataCAA GAACAGGTTT ATCGTGTCCA AAAACTCCAC CATATTCTAG 1141 AAATATTAGT CAGTTGCATG CCTTTCATTA AATCTCAACA TGAACTCCTC TTTTCTTTAA 1201 CACAGATCTG CATAAAGTAT TACAAACAAA ATCCTCTTGA TGAGCAACAC ATTTTTCAGC 1261 TGCCAGTCAG ACCAACTGCT GTAAAGAACT TATATCAAAG TGAGAAGCCA CAGAAATGGA 1321 GAGTGGAAAT ATATAGTGGT CAAAAGAAGA TTAAGACAGT TTGGCAACTG AGTGACAGCT 1381 CACCCATAGA CCATCTGAAT TTTCACAAAC CTGATTTTTC GGAATTAACA CTAAACGGTA 1441 GCCTGGAAGA AAGGATATTC TTTACTAACA TGGTTACCTG CAGCCAGGTG CATTTCAAGT 1501 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1561 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1621 TTTATATATC TTGTGGAAAG GACGAAAAGC AGGGGAGCTG CTTGTAGGCA CGCGTTAAGT 1681 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt