Construct: ORF TRCN0000477094
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005059.1_s317c1
- Derived from:
- ccsbBroadEn_07833
- DNA Barcode:
- GGAGTTACTATTCACATACCCATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ENDOD1 (23052)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477094
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23052 | ENDOD1 | endonuclease domain contain... | NM_015036.3 | 99.9% | 99.8% | 1483G>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1566
- ORF length:
- 1500
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg caccgcgcgc tggctcgcgc tgggcagcct cttcgccctg gctgggctgc 121 tggaaggccg gctcgtgggc gaggaggaag ccggctttgg cgaatgtgac aagttcttct 181 acgccgggac cccgcctgcg gggctggcgg ccgattccca cgtgaagatc tgtcagcgcg 241 cggagggtgc tgagcgcttc gccaccctct acagcacccg ggaccgcatc cccgtgtact 301 ccgcgttccg cgccccgcgc cctgcgcccg gcggcgccga gcagcgatgg ctggtggagc 361 cgcagatcga tgaccccaac agcaaccttg aggaggcgat taatgaggca gaggccatca 421 cctctgtgaa cagcctggga agcaagcaag ccttgaatac agattacctt gattctgatt 481 accaaagagg acagctttac ccattctccc ttagcagtga tgtccaggtg gccacattta 541 ctctcacaaa ttcagcccca atgactcagt ccttccagga acggtggtat gtgaatctcc 601 acagcctaat ggaccgggct ttgaccccac agtgtggcag tggggaagac ctatatatcc 661 tcacaggcac agtgccctca gactacagag ttaaagacaa agtggcagtc cctgagtttg 721 tttggctggc agcctgttgt gctgtccctg gaggaggctg ggccatgggc tttgtcaagc 781 acacccggga cagtgacatc atagaagatg tgatggtaaa agatcttcag aaactgcttc 841 catttaaccc tcagctgttt cagaacaact gtggtgaaac tgagcaagac acagagaaaa 901 tgaaaaaaat cctggaagtg gttaaccaaa tccaggatga agaacgaatg gtacaatctc 961 aaaagagttc tagtcccctt tctagcacca ggagcaagag gtctactctg ttgcctccag 1021 aggcatctga gggaagtagt agctttttgg gaaaactcat gggcttcatt gctaccccat 1081 tcatcaagct ttttcaatta atttattacc ttgtggtagc aatcctgaag aacattgtct 1141 atttcctgtg gtgtgttacc aagcaggtga ttaatggcat agaaagttgc ctttaccgcc 1201 tgggctcagc caccatctca tacttcatgg ccattgggga agagttggtg agcattccct 1261 ggaaggtgct caaggtcgtg gccaaagtca tcagggctct ccTCCGGATC CTTTGTTGTC 1321 TGCTGAAGGC CATTTGCCGA GTTCTGAGCA TCCCTGTCCG TGTCCTTGTG GATGTGGCCA 1381 CTTTCCCTGT GTACACCATG GGCGCTATTC CAATTGTTTG CAAGGACATT GCACTGGGCC 1441 TTGGTGGCAC TGTCTCACTG CTCTTTGACA CTGCTTTTGG TACCCTGGGT GGCCTATTTC 1501 AGGTGGTTTT TAGTGTCTGC AAGCGGATTG GCTACAAGGT TACTTTTTAC AATTCTGGGG 1561 AGTTATACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1621 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1681 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGGAGTTACT ATTCACATAC CCATTACGCG 1741 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt