Construct: ORF TRCN0000477096
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016139.1_s317c1
- Derived from:
- ccsbBroadEn_00625
- DNA Barcode:
- CAAATGAGACTATAAGTTACACGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GCNT1 (2650)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477096
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2650 | GCNT1 | glucosaminyl (N-acetyl) tra... | NM_001097633.1 | 100% | 100% | |
| 2 | human | 2650 | GCNT1 | glucosaminyl (N-acetyl) tra... | NM_001097634.1 | 100% | 100% | |
| 3 | human | 2650 | GCNT1 | glucosaminyl (N-acetyl) tra... | NM_001097635.1 | 100% | 100% | |
| 4 | human | 2650 | GCNT1 | glucosaminyl (N-acetyl) tra... | NM_001097636.1 | 100% | 100% | |
| 5 | human | 2650 | GCNT1 | glucosaminyl (N-acetyl) tra... | NM_001490.5 | 100% | 100% | |
| 6 | mouse | 14537 | Gcnt1 | glucosaminyl (N-acetyl) tra... | NM_001136484.3 | 83.5% | 85% | (many diffs) |
| 7 | mouse | 14537 | Gcnt1 | glucosaminyl (N-acetyl) tra... | NM_010265.5 | 83.5% | 85% | (many diffs) |
| 8 | mouse | 14537 | Gcnt1 | glucosaminyl (N-acetyl) tra... | NM_173442.5 | 83.5% | 85% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1353
- ORF length:
- 1284
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gctgaggacg ttgctgcgaa ggagactttt ttcttatccc accaaatact 121 actttatggt tcttgtttta tccctaatca ccttctccgt tttaaggatt catcaaaagc 181 ctgaatttgt aagtgtcaga cacttggagc ttgctgggga gaatcctagt agtgatatta 241 attgcaccaa agttttacag ggtgatgtaa atgaaatcca aaaggtaaag cttgagatcc 301 taacagtgaa atttaaaaag cgccctcggt ggacacctga cgactatata aacatgacca 361 gtgactgttc ttctttcatc aagagacgca aatatattgt agaacccctt agtaaagaag 421 aggcggagtt tccaatagca tattctatag tggttcatca caagattgaa atgcttgaca 481 ggctgctgag ggccatctat atgcctcaga atttctattg cattcatgtg gacacaaaat 541 ccgaggattc ctatttagct gcagtgatgg gcatcgcttc ctgttttagt aatgtctttg 601 tggccagccg attggagagt gtggtttatg catcgtggag ccgggttcag gctgacctca 661 actgcatgaa ggatctctat gcaatgagtg caaactggaa gtacttgata aatctttgtg 721 gtatggattt tcccattaaa accaacctag aaattgtcag gaagctcaag ttgttaatgg 781 gagaaaacaa cctggaaacg gagaggatgc catcccataa agaagaaagg tggaagaagc 841 ggtatgaggt cgttaatgga aagctgacaa acacagggac tgtcaaaatg cttcctccac 901 tcgaaacacc tctcttttct ggcagtgcct ACTTCGTGGT CAGTAGGGAG TATGTGGGGT 961 ATGTACTACA GAATGAAAAA ATCCAAAAGT TGATGGAGTG GGCACAAGAC ACATACAGCC 1021 CTGATGAGTA TCTCTGGGCC ACCATCCAAA GGATTCCTGA AGTCCCGGGC TCACTCCCTG 1081 CCAGCCATAA GTATGATCTG TCTGACATGC AAGCAGTTGC CAGGTTTGTC AAGTGGCAGT 1141 ACTTTGAGGG TGATGTTTCC AAGGGTGCTC CCTACCCGCC CTGCGATGGA GTCCATGTGC 1201 GCTCAGTGTG CATTTTCGGA GCTGGTGACT TGAACTGGAT GCTGCGCAAA CACCACTTGT 1261 TTGCCAATAA GTTTGACGTG GATGTTGACC TCTTTGCCAT CCAGTGTTTG GATGAGCATT 1321 TGAGACACAA AGCTTTGGAG ACATTAAAAC ACTTGCCAAC TTTCTTGTAC AAAGTGGTTG 1381 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1441 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACA 1501 AATGAGACTA TAAGTTACAC GTACGCGTTA AGTCgacaat caacctctgg attacaaaat 1561 ttgtgaaaga tt