Construct: ORF TRCN0000477103
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018273.1_s317c1
- Derived from:
- ccsbBroadEn_10623
- DNA Barcode:
- CGCCAACAAGCAGGGGTACTCGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LOC389834 (389834)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477103
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 389834 | LOC389834 | ankyrin repeat domain 57 ps... | NR_027420.1 | 8.8% | 1_835del;1565_8205del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 798
- ORF length:
- 729
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcggagcgca gcattcacca tcccctccct gaaacagcgg tccccgaggt 121 gctccacagg cagggccgag ctgggcaagg gggagcccag cccctgcacg ggccgccctg 181 agcagcgggg acgcaggaag agctcgctgg ctccaccagc ccctacccca gatgcgggac 241 ctcagaccag caaggacctg gagcccccac cccacggttg ccaggaggcg gacaggggcg 301 gctcctgggg ggctaccacc tcgaggccgt tccgccagaa cttgagcgac ttgggaaggc 361 acagtgtcct gcccttgaag aggaacctgt gtcctggagg cagcagcctg ggagctcctc 421 ctctgaggac accgcagagg cgagtgactc tGGCGGCGCA GCGCTGGCTT TCCCGTCCGC 481 AGAGGAGAGC TGTGGGGCTG GGTGAGCTGG ACCAGGGAGC ACAGCTGGCT GCTCTCGGCC 541 TCCGATGGGG AGTGGACAGC TTAGGGGGTT GCCCCCGTGC CAGCCAGCCT GCTGGCCACT 601 CTGGGCTTCA TCACACCCTC ACCTGCCTGC GCAGGCACCT AGCACTGCAG GCTGGAGCTT 661 CTGGCCATGC TGGTCAACTT CCCCAACGAG CCTCTGCTGC CTGGGAACAG CAAGGCCAGA 721 GCTACACCGC CCTGCACTTG GCAGCCATGT ACCTTGGAGA TGGTGAAGCT GCTAGTGGGA 781 ACATAGGACG CCGATGTTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 841 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 901 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACGCCAAC AAGCAGGGGT 961 ACTCGTGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt