Construct: ORF TRCN0000477106
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010654.1_s317c1
- Derived from:
- ccsbBroadEn_10179
- DNA Barcode:
- CCATCGTGCTTAGCACGAGCATCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MS4A13 (503497)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477106
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 503497 | MS4A13 | membrane spanning 4-domains... | NM_001012417.3 | 99.7% | 99.3% | 19A>G |
| 2 | human | 503497 | MS4A13 | membrane spanning 4-domains... | NM_001100909.2 | 73.4% | 73% | 19A>G;185_186ins120 |
| 3 | human | 503497 | MS4A13 | membrane spanning 4-domains... | NM_001278320.2 | 60.9% | 60.5% | 19A>G;129_130ins177 |
| 4 | human | 503497 | MS4A13 | membrane spanning 4-domains... | XR_949954.2 | 45.7% | 1_354del;373A>G;811_994del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 525
- ORF length:
- 456
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gattggcatc tttcacgttt tcatgtggta ctttctattg gttttgtata 121 tgggacaaat taaaGGAGCC TTTGGAACGT ATGAACCTGT AACTTACAAA ACAGGATGTA 181 CTTTATGGGG AATTTTTTTT ATCATTGCAG GAGTCTTCCT AATAAGAGTA ACAAAGTATC 241 CGACTCGATC TGGAATTATA AGTACTTTAA TCATAAACAT CATCTGCATA ATTACTACAA 301 TTACTGCAGT AACTCTAACA ATAATAGAGT TGTCTCATTT TAATTCTGTG TCATACAGGA 361 ATTATGGACA AGCAAAACTT GGGAGGGAAG TATCACGTAT TTTACTGTTC TTCTACGGTT 421 TGGAATTTTC TATTGCACTT ACACACTCAA TATACAGCTG TTCCAATTTG TTTCGAAGAC 481 AAAATGATCT TACATCTGTT ACTGAGGAAG CTGAGAGCAC TCCTTTGCCA ACTTTCTTGT 541 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 601 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 661 GAAAGGACGA CCATCGTGCT TAGCACGAGC ATCTACGCGT TAAGTCgaca atcaacctct 721 ggattacaaa atttgtgaaa gatt