Construct: ORF TRCN0000477124
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016372.1_s317c1
- Derived from:
- ccsbBroadEn_07908
- DNA Barcode:
- ACTCTTGCAGACGAATGCTCCTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PADI4 (23569)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477124
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23569 | PADI4 | peptidyl arginine deiminase 4 | NM_012387.3 | 99.6% | 99.5% | (many diffs) |
| 2 | human | 23569 | PADI4 | peptidyl arginine deiminase 4 | XM_011541150.1 | 90.3% | 90.1% | (many diffs) |
| 3 | human | 23569 | PADI4 | peptidyl arginine deiminase 4 | XM_011541151.1 | 84.6% | 84.4% | (many diffs) |
| 4 | human | 23569 | PADI4 | peptidyl arginine deiminase 4 | XM_011541152.1 | 72.9% | 73% | 0_1ins537;552G>A;612C>A |
| 5 | human | 23569 | PADI4 | peptidyl arginine deiminase 4 | XM_011541155.1 | 58.5% | 57.5% | (many diffs) |
| 6 | human | 23569 | PADI4 | peptidyl arginine deiminase 4 | XM_011541153.1 | 58.4% | 57.8% | (many diffs) |
| 7 | human | 23569 | PADI4 | peptidyl arginine deiminase 4 | XM_011541154.2 | 58.4% | 57.8% | (many diffs) |
| 8 | human | 23569 | PADI4 | peptidyl arginine deiminase 4 | XM_011541156.1 | 57.8% | 57.6% | (many diffs) |
| 9 | human | 23569 | PADI4 | peptidyl arginine deiminase 4 | XM_011541157.1 | 55.1% | 55.2% | 0_1ins891;198G>A;258C>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2055
- ORF length:
- 1989
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ccaggggaca ttgatccgtg tgaccccaga gcagcccacc catgccgtgt 121 gtgtgctggg caccttgact cagcttgaca tctgcagctc tgcccctgag gactgcacgt 181 ccttcagcat caacgcctcc ccaggggtgg tcgtggatat tgcccacagc cctccagcca 241 agaagaaatc cacaggttcc tccacatggc ccctggaccc tggggtagag gtgaccctga 301 cgatgaaagc ggccagtggt agcacaggcg accagaaggt tcagatttca tactacggac 361 ccaagactcc accagtcaaa gctctactct acctcaccgc ggtggaaatc tccctgtgcg 421 cagacatcac ccgcaccggc aaagtgaagc caaccagagc tgtgaaagat cagaggacct 481 ggacctgggg cccttgtgga cagggtgcca tcctgctggt gaactgtgac agagacaatc 541 tcgaatcttc tgccatggac tgcgaggatg atgaagtgct tgacagcgaa gacctgcagg 601 acatgtcgct gatgaccctg agcacgaaga cccccaagga cttcttcaca aaccatacac 661 tggtgctcca cgtggccagg tctgagatgg acaaagtgag ggtgtttcag gccacacggg 721 gcaaactgtc ctccaagtgc agcgtagtct tgggtcccaa gtggccctct cactacctga 781 tggtccccgg tggaaagcac aacatggact tctacgtgga ggccctcgct ttcccggaca 841 ccgacttccc ggggctcatt accctcacca tctccctgct ggacacgtcc aacctggagc 901 tccccgaggc tgtggtgttc caagacagcg tggtcttccg cgtggcgccc tggatcatga 961 cccccaacac ccagcccccg caggaggtgt acgcgtgcag tatttttgaa aatgaggact 1021 tcctgaagtc agtgactact ctggccatga aagccaagtg caagctgacc atctgccctg 1081 aggaggagaa catggatgac cagtggatgc aggatgaaat ggagatcggc tacatccaag 1141 ccccacacaa aacactgccc gtggtcttcg actctccaag gaacagaggc ctgaaggagt 1201 ttcccatcaa acgagtgatg ggtccagatt ttggctatgt aactcgaggg ccccaaacag 1261 ggggtatcag tggactggac tcctttggga acctggaagt gagcccccca gtcacagtca 1321 ggggcaagga atacccgctg ggcaggattc tcttcgggga cagctgttat cccagcaatg 1381 acagccggca gatgcaccag gccctgcagg acttcctcag tgcccagcag gtgcaggccc 1441 ctgtgaagct ctattctgac tggctgtccg tgggccacgt ggacgagttc ctgagctttg 1501 tgccagcacc cgacaggaag ggcttccggc tgctcctggc cagccccagg tcctgctaca 1561 aactgttcca ggagcagcag aatgagggcc acggggaggc cctgctgttc gaagggatca 1621 agaaaaaaaa acagcagaaa ataaagaaCA TTCTGTCAAA CAAGACATTG AGAGAACATA 1681 ATTCATTTGT GGAGAGATGC ATCGACTGGA ACCGCGAGCT GCTGAAGCGG GAGCTGGGCC 1741 TGGCCGAGAG TGACATCATT GACATCCCGC AGCTCTTCAA GCTCAAAGAG TTCTCTAAGG 1801 CGGAAGCTTT TTTCCCCAAC ATGGTGAACA TGCTGGTGCT AGGGAAGCAC CTGGGCATCC 1861 CCAAGCCCTT CGGGCCCGTC ATCAACGGCC GCTGCTGCCT GGAGGAGAAG GTGTGTTCCC 1921 TGCTGGAGCC ACTGGGCCTC CAGTGCACCT TCATCAACGA CTTCTTCACC TACCACATCA 1981 GGCATGGGGA GGTGCACTGC GGCACCAACG TGCGCAGAAA GCCCTTCTCC TTCAAGTGGT 2041 GGAACATGGT GCCCTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 2101 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 2161 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA ACTCTTGCAG ACGAATGCTC 2221 CTGCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt