Construct: ORF TRCN0000477128
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012032.1_s317c1
- Derived from:
- ccsbBroadEn_15384
- DNA Barcode:
- CCTACATCAGCCTTACAGTTCGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHRNB3 (1142)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477128
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1142 | CHRNB3 | cholinergic receptor nicoti... | NM_000749.5 | 99.7% | 100% | 19C>T;615C>T;1209G>C |
| 2 | human | 1142 | CHRNB3 | cholinergic receptor nicoti... | NM_001347717.1 | 83.6% | 83.8% | 0_1ins222;393C>T;987G>C |
| 3 | human | 1142 | CHRNB3 | cholinergic receptor nicoti... | XM_011544390.2 | 71.6% | 71.8% | 0_1ins387;228C>T;822G>C |
| 4 | mouse | 108043 | Chrnb3 | cholinergic receptor, nicot... | NM_173212.4 | 79.3% | 84.9% | (many diffs) |
| 5 | mouse | 108043 | Chrnb3 | cholinergic receptor, nicot... | NM_027454.4 | 76.4% | 81.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1440
- ORF length:
- 1374
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct cccagatttt atgttggttc tcatcgtcct tggcatccct tcctcagcca 121 ccacaggttt caactcaatc gccgaaaatg aagatgccct cctcagacat ttgttccaag 181 gttatcagaa atgggtccgc cctgtattac attctaatga caccataaaa gtatattttg 241 gattgaaaat atcccagctt gtagatgtgg atgaaaagaa tcagctgatg acaaccaatg 301 tgtggctcaa acaggaatgg acagaccaca agttacgctg gaatcctgat gattatggtg 361 ggatccattc cattaaagtt ccatcagaat ctctgtggct tcctgacata gttctctttg 421 aaaatgctga cggccgcttc gaaggctccc tgatgaccaa ggtcatcgtg aaatcaaacg 481 gaactgttgt ctggacccct cccgccagct acaaaagctc ctgcaccatg gacgtcacgt 541 ttttcccgtt cgaccgacag aactgctcca tgaagtttgg atcctggact tatgatggca 601 ccatggttga cctcattttg atcaatgaaa atgtcgacag aaaagacttc ttcgataacg 661 gagaatggga aatactgaat gcaaagggga tgaaggggaa cagaagggac ggcgtgtact 721 cctatccctt tatcacgtat tccttcgtcc tgagacgcct gcctttattc tataccctct 781 ttctcatcat cccctgcctg gggctgtctt tcctaacagt tcttgtgttc tatttacctt 841 cggatgaagg agaaaaactt tcattatcca catcggtctt ggtttctctg acagttttcc 901 ttttagtgat tgaagaaatc atcccatcgt cttccaaagt cattcctctc attggagagt 961 acctgctgtt catcatgatt tttgtgaccc tgtccatcat tGTTACCGTG TTTGTCATTA 1021 ACGTTCACCA CAGATCTTCT TCCACGTACC ACCCCATGGC CCCCTGGGTT AAGAGGCTCT 1081 TTCTGCAGAA ACTTCCAAAA TTACTTTGCA TGAAAGATCA TGTGGATCGC TACTCATCCC 1141 CAGAGAAAGA GGAGAGTCAA CCAGTAGTGA AAGGCAAAGT CCTCGAAAAA AAGAAACAGA 1201 AACAGCTTAG TGATGGAGAA AAAGTTCTAG TTGCTTTTTT GGAAAAAGCT GCTGATTCCA 1261 TTAGATACAT TTCCAGACAT GTGAAGAAAG AACATTTTAT CAGCCAGGTA GTACAAGACT 1321 GGAAATTTGT AGCTCAAGTT CTTGACCGAA TCTTCCTGTG GCTCTTTCTG ATAGTGTCAG 1381 TAACAGGCTC GGTTCTGATT TTTACCCCTG CTTTGAAGAT GTGGCTACAT AGTTACCATT 1441 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1501 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1561 TTTATATATC TTGTGGAAAG GACGACCTAC ATCAGCCTTA CAGTTCGTGA CGCGTTAAGT 1621 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt