Transcript: Human XM_011544390.2

PREDICTED: Homo sapiens cholinergic receptor nicotinic beta 3 subunit (CHRNB3), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
CHRNB3 (1142)
Length:
2158
CDS:
380..1369

Additional Resources:

NCBI RefSeq record:
XM_011544390.2
NBCI Gene record:
CHRNB3 (1142)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011544390.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000304031 ACGCTGGAATCCTGATGATTA pLKO_005 262 5UTR 100% 13.200 18.480 N CHRNB3 n/a
2 TRCN0000061262 CCTATCCCTTTATCACGTATT pLKO.1 648 CDS 100% 10.800 15.120 N CHRNB3 n/a
3 TRCN0000331351 ATGGGAGAAACTCTGGTAAAT pLKO_005 1502 3UTR 100% 13.200 10.560 N CHRNB3 n/a
4 TRCN0000370043 GTAGTACAAGACTGGAAATTT pLKO_005 1235 CDS 100% 15.000 10.500 N CHRNB3 n/a
5 TRCN0000304094 TGACCAAGGTCATCGTGAAAT pLKO_005 381 CDS 100% 13.200 9.240 N CHRNB3 n/a
6 TRCN0000377445 CATCGTCTTCCAAAGTCATTC pLKO_005 852 CDS 100% 10.800 7.560 N CHRNB3 n/a
7 TRCN0000061261 GCTGATTCCATTAGATACATT pLKO.1 1178 CDS 100% 5.625 3.938 N CHRNB3 n/a
8 TRCN0000061259 GCTTTGAAGATGTGGCTACAT pLKO.1 1337 CDS 100% 4.950 3.465 N CHRNB3 n/a
9 TRCN0000061258 GCCTTTATTCTATACCCTCTT pLKO.1 688 CDS 100% 4.050 2.835 N CHRNB3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011544390.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06003 pDONR223 100% 71.6% 71.8% None 0_1ins387;228C>T;822G>C n/a
2 ccsbBroad304_06003 pLX_304 0% 71.6% 71.8% V5 0_1ins387;228C>T;822G>C n/a
3 TRCN0000476858 AATCTGCGAAGGACGCACACCTCT pLX_317 34.9% 71.6% 71.8% V5 0_1ins387;228C>T;822G>C n/a
4 ccsbBroadEn_15384 pDONR223 0% 71.6% 71.8% None 0_1ins387;228C>T;822G>C n/a
5 ccsbBroad304_15384 pLX_304 0% 71.6% 71.8% V5 0_1ins387;228C>T;822G>C n/a
6 TRCN0000477128 CCTACATCAGCCTTACAGTTCGTG pLX_317 32.2% 71.6% 71.8% V5 0_1ins387;228C>T;822G>C n/a
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