Construct: ORF TRCN0000477130
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016015.1_s317c1
- Derived from:
- ccsbBroadEn_08712
- DNA Barcode:
- GAATTGTTCGAGTCTCTATTCGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM63C (57156)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477130
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57156 | TMEM63C | transmembrane protein 63C | NM_020431.4 | 99.9% | 100% | 633A>G |
| 2 | mouse | 217733 | Tmem63c | transmembrane protein 63c | XM_006515727.3 | 52.4% | 52.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2487
- ORF length:
- 2418
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtctgcctca ccagacgacc tgagtacagg gggaaggtta cagaacatga 121 cagtggatga atgcttccag tctcggaaca ccgtcctcca ggggcagccc tttgggggtg 181 tccccaccgt gctgtgcctc aacatcgccc tgtgggtgct cgtccttgtg gtttactcct 241 tcctccggaa agctgcgtgg gactatgggc gcctggctct gctgatacac aatgacagcc 301 tgacctcgct gatctatggg gagcagagcg agaagacatc tccctcggag acttccttgg 361 agatggaacg cagagacaag ggattctgtt cctggttctt caacagcata acaatgaagg 421 acgaggatct gattaacaag tgtggggacg acgcgcgcat ctacatcgtg ttccagtacc 481 acctcatcat ctttgtgctc atcatctgta tcccctccct gggcatcatt ttgcccatca 541 actatactgg atctgttctg gactggagca gtcactttgc tcggaccacc attgtcaatg 601 tctccacaga gagcaagctc ctgtggctgc atagcctgct gtccttcttc tacttcatca 661 ccaacttcat gttcatggct catcactgcc tggggtttgc gcccaggaat agccaaaagg 721 tcacaaggac actaatgatc acctatgtgc ccaaggacat tgaagaccca gaactcatca 781 ttaagcattt tcacgaggcc tatccaggca gtgtcgtgac aagagtccac ttctgctacg 841 acgtcaggaa cctgatcgac ttggacgatc agaggcgcca tgccatgcgg ggccggcttt 901 tctatacagc caaggccaag aagactggga aggtgatgat caggatccac ccctgtgccc 961 gcctgtgctt ctgcaagtgc tggacctgct tcaaggaggt ggatgcagag cagtattaca 1021 gcgagctaga ggagcagcta acggacgagt tcaacgccga gctcaaccgc gtgccgctca 1081 agcggctgga cctgatcttt gtcaccttcc aggactccag gatggccaag cgtgtccgta 1141 aggattacaa gtatgtccag tgtggtgtgc aaccccagca gtcctcagtg accaccatcg 1201 tcaaatcata ttactggagg gtcactatgg ccccacaccc caaagacatt atttggaaac 1261 acctgtctgt ccgccgcttc ttttggtggg cccgctttat cgcaatcaac accttcctct 1321 tcttcctctt cttctttctc accacgcctg ccatcatcat gaacactatc gacatgtaca 1381 acgtcacccg ccccatcgag aagctgcaga acccaattgt gacccagttc ttcccctctg 1441 tgatgctctg gggcttcaca gtgatactgc ctctgattgt ctacttctcc gccttcctcg 1501 aggcccactg gaccagatca agtcagaatc tggtcatggt gcacaagtgc tacatctttc 1561 tggtgttcat ggtagtcatt ctgccctcta tgggactgac cagtttggat gtctttctcc 1621 gctggctctt tgacatctac tatctagagc aagcatccat caggttccag tgtgtgttcc 1681 tgccagacaa cggcgccttc tttgtcaact acgtgatcac ggcagcttta cttggcacag 1741 gcatggagct gctgcgtctg gggtcactct tctgctacag cacccgcctc ttcttctcta 1801 gatcagagcc agagagagtc aacatcagaa agaaccaggc catagacttc cagtttgggc 1861 gtgagtatgc gtggatgatg aacgtgttca gcgtggtgat ggcgtacagc atcacttgcc 1921 ccatcattgt gccttttggg ttgctctacc tgtgcatgaa gcacttgacg gatcgctata 1981 acatgtacta ctcctttgca cccaccaaac tgaacgagca gatccacatg gctgccgtct 2041 cccaggccat ctttgcgcca ctcttgggtc tgttctggat gctgttcttc tccatcctgc 2101 ggttgggttc tctccacgcc atcaccatct tttccctgtc caccctcctc attgccatgg 2161 tgattgcctt tgttggcatt tttctgggga agcttcggat ggttgccGAC TACGAGCCCG 2221 AGGAGGAGGA GATCCAGACA GTGTTTGACA TGGAGCCAAG CAGCACCTCC TCCACGCCCA 2281 CCTCCCTCCT GTATGTGGCC ACCGTGCTGC AAGAACCGGA GTTGAATCTG ACCCCCGCCT 2341 CCTCCCCAGC CAGGCACACC TATGGCACCA TGAACAACCA GCCGGAAGAG GGAGAAGAAG 2401 AGAGTGGTCT GAGGGGCTTT GCGAGGGAGC TAGACTCGGC CCAGTTCCAG GAAGGGCTGG 2461 AACTGGAGGG CCAGAACCAG TACCACTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 2521 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 2581 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGAATTGTT 2641 CGAGTCTCTA TTCGCGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 2701 aagatt