Construct: ORF TRCN0000477136
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008147.1_s317c1
- Derived from:
- ccsbBroadEn_09762
- DNA Barcode:
- TCAACCCAGCTGCACTGACCAATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RNF168 (165918)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477136
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 165918 | RNF168 | ring finger protein 168 | NM_152617.4 | 99.9% | 99.8% | 14A>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1779
- ORF length:
- 1713
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tctacccata gacgccatcc cctcgctgtc cgagtgccag tgcgggatct 121 gcatggaaat cctcgtggag cccgtcaccc tcccgtgtaa ccacacgctg tgtaaaccgt 181 gcttccagtc gaccgtcgaa aaggcgagtt tatgctgtcc cttctgtcgc cgccgggtat 241 cgtcgtggac tcggtaccat acccgaagaa attctctcgt caacgtggaa ctgtggacga 301 taattcaaaa acactatccc agggagtgca agcttagagc gtctggccaa gaatcagagg 361 aagtggctga tgactatcag ccagttcgtc tgctcagtaa acctggggaa ctgagaagag 421 aatatgaaga ggaaataagc aaggtggcgg cagagcgacg ggccagcgag gaagaagaaa 481 acaaagccag tgaagaatac atacagaggt tgttggcaga ggaggaagaa gaggaaaaaa 541 gacaggcaga aaaaaggcga agagcgatgg aagaacaact gaaaagtgat gaggaactgg 601 caagaaagct aagcattgat attaacaatt tctgtgaggg aagtatctcg gcttctccct 661 tgaattccag aaaatctgat ccagttacac ccaagtctga aaagaaaagt aagaacaaac 721 aaagaaacac tggagatatt cagaagtatt tgacaccgaa atctcagttt gggtcagcct 781 cacactctga agctgtacaa gaagtcagga aagactccgt atctaaggac attgacagta 841 gtgataggaa aagcccaaca gggcaagaca cagaaataga agatatgccg acactttctc 901 cacagatatc ccttggagtt ggagaacaag gtgcagattc ttcaatagag tcccctatgc 961 catggttatg tgcctgtggt gccgaatggt accatgaagg aaacgtcaaa acaagaccaa 1021 gcaatcatgg gaaagagtta tgtgtcttaa gtcacgagcg acctaaaacc agagttccct 1081 actcgaaaga aactgcagtt atgccttgtg gcagaacaga aagtgggtgc gcccccacat 1141 caggggtgac acagacaaat ggaaacaaca caggtgagac agaaaatgaa gagtcgtgcc 1201 tactgatcag taaggagatt tccaaaagaa aaaaccaaga atcttccttt gaagcagtca 1261 aggatccatg cttttctgca aaaagaagaa aagtgtcccc cgaatcttcc ccagatcaag 1321 aggaaacaga aataaacttt acccaaaaac tgatagattt ggagcatcta ctgtttgaga 1381 gacataaaca agaagaacag gacaggttat tGGCATTACA ACTTCAGAAG GAGGTGGATA 1441 AAGAGCAAAT GGTGCCAAAC CGGCAAAAAG GATCCCCAGA TGAGTATCAC TTACGCGCTA 1501 CATCCTCCCC TCCAGACAAA GTGCTAAATG GACAGAGGAA GAATCCCAAA GATGGGAACT 1561 TCAAAAGGCA AACTCACACA AAGCATCCAA CACCAGAGAG AGGCTCAAGG GACAAAAATA 1621 GGCAAGTGTC TTTAAAGATG CAGTTGAAGC AGTCAGTTAA TAGAAGAAAG ATGCCAAATT 1681 CTACTAGAGA TCACTGTAAG GTATCCAAAA GTGCTCACTC CCTACAGCCT AGCATTTCAC 1741 AGAAAAGTGT TTTTCAGATG TTTCAGAGAT GCACAAAGTA CCCAACTTTC TTGTACAAAG 1801 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1861 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1921 ACGATCAACC CAGCTGCACT GACCAATTAC GCGTTAAGTC gacaatcaac ctctggatta 1981 caaaatttgt gaaagatt