Construct: ORF TRCN0000477141
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013970.1_s317c1
- Derived from:
- ccsbBroadEn_04544
- DNA Barcode:
- GGAGAACCTTTACATCTGCAATCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RCCD1 (91433)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477141
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 91433 | RCCD1 | RCC1 domain containing 1 | NM_001017919.1 | 100% | 100% | |
| 2 | human | 91433 | RCCD1 | RCC1 domain containing 1 | NM_033544.2 | 100% | 100% | |
| 3 | human | 91433 | RCCD1 | RCC1 domain containing 1 | XR_001751415.1 | 22.7% | 1_208del;1158_1683del;1863_4954del | |
| 4 | human | 91433 | RCCD1 | RCC1 domain containing 1 | XR_001751417.1 | 22.7% | 1_223del;1173_1698del;1878_4969del | |
| 5 | human | 91433 | RCCD1 | RCC1 domain containing 1 | XR_001751414.1 | 22.4% | 1_284del;1234_1759del;1939_5030del | |
| 6 | human | 91433 | RCCD1 | RCC1 domain containing 1 | XR_001751416.1 | 22.3% | 1_307del;1257_1782del;1962_5053del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1197
- ORF length:
- 1128
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggaggag cggccggggg cctggttcgg cttcggtttc tgcggcttcg 121 ggcaggagct gggctccgga cgcgggcgcc aggtgcacag ccccagtccg ctgcgggcgg 181 gcgtcgacat ctgccgcgtg agcgcgagct ggagctacac cgctttcgtg acccgtggag 241 gccgcttgga gctgtcgggc tcagccagcg gcgcggcggg ccgctgcaag gacgcgtggg 301 cctcggaggg gctcctcgcg gtgctgcgcg ccgggccggg gccggaggcg ttactgcagg 361 tctgggcggc cgaatcggcg ctgcgtgggg agccattgtg ggcccagaat gtggtgcccg 421 aggccgaagg ggaagacgat ccggccggtg aggcccaggc tgggaggcta cccctgctgc 481 cctgcgcccg tgcctacgtg agcccgcggg cgcccttcta ccggcctctg gctccggagc 541 tgcgggcacg ccagctggag ctgggcgccg agcacgcgtt gctgctggac gctgctggcc 601 aggtgttctc ctggggcggg ggcaggcatg gacagctggg ccatgggacc ctggaggcag 661 agctggagcc acggctgttg gaggcgttgc agggcctagt catggctgag gtggccgcgg 721 ggggctggca ttctgtgtgt gtgagtgaga ctggggatat ttatatctgg ggctggaatg 781 aatcagggca gctggccctg cccaccagga acctggcaga ggatggagag acTGTCGCAA 841 GGGAAGCCAC AGAACTGAAT GAAGATGGTT CTCAGGTGAA GAGAACGGGT GGGGCTGAGG 901 ATGGAGCCCC TGCCCCCTTC ATAGCTGTCC AGCCCTTCCC GGCATTACTG GATCTCCCCA 961 TGGGCTCAGA TGCAGTCAAG GCCAGCTGTG GATCCCGGCA CACAGCTGTG GTGACACGAA 1021 CAGGGGAGCT CTACACCTGG GGCTGGGGTA AATATGGACA GCTGGGCCAC GAGGACACCA 1081 CCAGCTTGGA TCGGCCTCGC CGTGTGGAAT ACTTTGTAGA TAAGCAACTC CAAGTAAAGG 1141 CTGTCACCTG TGGGCCGTGG AACACCTACG TGTATGCTGT GGAGAAAGGG AAGAGCTTGC 1201 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1261 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1321 ATATATCTTG TGGAAAGGAC GAGGAGAACC TTTACATCTG CAATCCACGC GTTAAGTCga 1381 caatcaacct ctggattaca aaatttgtga aagatt