Construct: ORF TRCN0000477155
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002746.1_s317c1
- Derived from:
- ccsbBroadEn_10429
- DNA Barcode:
- CGTCGACTTCACATCGCGCGATCT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FMO4 (2329)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477155
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2329 | FMO4 | flavin containing dimethyla... | NM_002022.3 | 99.8% | 99.8% | 1668_1669delGC |
| 2 | human | 2329 | FMO4 | flavin containing dimethyla... | XM_005245045.3 | 90.9% | 90.8% | 0_1ins150;1518_1519delGC |
| 3 | human | 2329 | FMO4 | flavin containing dimethyla... | XM_005245046.3 | 88.5% | 88.5% | 132_133ins189;1479_1480delGC |
| 4 | human | 2329 | FMO4 | flavin containing dimethyla... | XM_011509347.2 | 80.1% | 74.1% | (many diffs) |
| 5 | human | 2329 | FMO4 | flavin containing dimethyla... | XM_011509348.2 | 74.2% | 74.1% | 0_1ins429;1239_1240delGC |
| 6 | human | 2329 | FMO4 | flavin containing dimethyla... | XM_006711243.3 | 68.1% | 68.1% | 483_484ins531;1137_1138delGC |
| 7 | human | 2329 | FMO4 | flavin containing dimethyla... | XR_002959842.1 | 59.2% | 1_431del;1257_1258ins188;1678_1679ins237 | |
| 8 | human | 2329 | FMO4 | flavin containing dimethyla... | XM_005245048.3 | 58.3% | 58.2% | 483_484ins696;972_973delGC |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1740
- ORF length:
- 1674
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc caagaaagtt gcagtgattg gagctggtgt gagtggcctc tcctccatca 121 aatgctgtgt ggatgaggac ctggagccca cctgctttga gagaagtgat gacattgggg 181 gattatggaa gtttactgaa tcttccaaag atgggatgac cagggtctat aagtcattag 241 tgacaaatgt ctgtaaggaa atgtcatgtt acagtgactt ccctttccac gaagattatc 301 ctaatttcat gaaccatgaa aaattttggg actatctcca agaatttgct gagcactttg 361 acctcctgaa atacattcag tttaagacca ctgtgtgcag cataacgaag cgtccagact 421 tctccgaaac tggtcagtgg gatgttgtca cagagacaga gggcaagcaa aatagagctg 481 tctttgatgc tgttatggtt tgcactggac atttcctgaa tccccattta cctttggaag 541 cctttcctgg aattcataag tttaaaggtc agatcctgca tagtcaagag tacaagatcc 601 cagaaggctt tcagggcaaa cgcgtcttgg tgattggtct tgggaacact ggaggagaca 661 ttgctgtgga actcagtcga acggcagctc aggtacttct cagtactaga actggtacct 721 gggttcttgg gcgctcttca gattggggct atccttataa tatgatggtt acaagaagat 781 gctgtagttt tattgcacaa gttctgcctt cacgttttct aaactggatt caagaaagga 841 agttgaataa gagatttaat catgaggatt atggattaag tattaccaaa gggaaaaaag 901 caaaattcat tgtgaatgat gagctgccaa actgtatcct ctgtggggca atcactatga 961 aaaccagcgt gattgaattt acagaaacct ctgctgtctt tgaagatggg acagtggaag 1021 aaaacattga tgttgtgatc ttcactacag gatatacatt ttcttttcca ttttttgaag 1081 aacctcttaa aagcctctgt acaaagaaga tatttctata caagcaagtc tttcccttaa 1141 acctagagag agcgacatta gccatcatcg gccttatcgg ccttaaagga tccatcttat 1201 caggcacaga gctccaagca cgatgggtca caagagtatt caaaggactc tgtaagatac 1261 ctccatccca aaaattgatg atggaggcta ctgaaaagga acagctcatt aaaaggggag 1321 tgtttaaaga caccagcaaa gacaaatttg actacattgc ctacatgGAT GATATCGCTG 1381 CCTGCATAGG CACAAAGCCC AGCATCCCAC TTCTGTTCCT CAAGGATCCC AGACTAGCTT 1441 GGGAAGTTTT CTTTGGACCA TGTACTCCTT ATCAGTACCG CCTCATGGGC CCTGGAAAAT 1501 GGGATGGAGC CAGAAATGCC ATCCTGACCC AGTGGGACAG AACATTGAAA CCTTTAAAAA 1561 CTCGAATTGT CCCTGATTCC TCCAAGCCTG CCTCCATGTC ACATTATTTA AAAGCCTGGG 1621 GGGCACCTGT CCTACTTGCC TCTCTTCTAC TTATCTGTAA ATCTTCACTT TTCTTGAAAT 1681 TGGTGAGAGA TAAACTACAG GACAGAATGT CCCCTTACCT AGTAAGTCTT TGGAGGATAC 1741 CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT 1801 CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT 1861 TATATATCTT GTGGAAAGGA CGACGTCGAC TTCACATCGC GCGATCTACG CGTTAAGTCg 1921 acaatcaacc tctggattac aaaatttgtg aaagatt