Construct: ORF TRCN0000477169
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013747.1_s317c1
- Derived from:
- ccsbBroadEn_02906
- DNA Barcode:
- TCCTTTGTCAGCGACACATACGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IPCEF1 (26034)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477169
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26034 | IPCEF1 | interaction protein for cyt... | NM_015553.2 | 100% | 100% | |
| 2 | human | 26034 | IPCEF1 | interaction protein for cyt... | NM_001130699.1 | 99.7% | 99.7% | 37_39delCAG |
| 3 | human | 26034 | IPCEF1 | interaction protein for cyt... | NM_001130700.1 | 99.7% | 99.7% | 37_39delCAG |
| 4 | mouse | 320495 | Ipcef1 | interaction protein for cyt... | XM_017313969.1 | 35.4% | 34% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1380
- ORF length:
- 1311
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gacatcatac atggctattg atggcagtgc tcttgttccc ttgcgtcaga 121 agcccaggag gaaaactcaa ggttttctca cgatgagtcg gaggaggata tcgtgtaaag 181 atctgggcca tgctgactgc caagggtggc tgtataagaa aaaggaaaag ggaagtttcc 241 taagcaacaa atggaaaaag ttctgggtga tactgaaggg gtcgtcactg tactggtata 301 gcaatcaaat ggcagagaaa gctgatggat ttgtcaacct gcctgatttc actgtggaaa 361 gagcatctga atgcaagaaa aagcatgctt ttaagatcag ccatccacag atcaagacct 421 tttattttgc agctgagaat gtgcaggaaa tgaacgtgtg gttaaataaa cttggatcgg 481 ctgtaatcca tcaggaatcc actacaaagg atgaagaatg ttacagtgaa agtgaacagg 541 aagatccaga aatagctgcg gagacaccac cccctcctca cgcttcccag actcagtctt 601 tgactgcaca gcaggcatct tcatcctcac ccagcctgag tggaacgtcg tattctttct 661 cttccctgga aaatacagtg aagacaccca gcagttttcc ttcctcctta tctaaagaga 721 gacaatcctt gcctgacaca gttaacagtt tgtctgctgc tgaagatgag ggacaaccaa 781 taacgtttgc tgtgcaagtt cattcacctg taccctcaga ggcaggcatc cacaaggccc 841 tggaaaacag ttttgtcaca tcagaaagtg gatttttgaa ctctttatct agtgatgata 901 cttcttcatt gagtagcaat catgaccatc ttactgtccc agataagcct gctGGATCAA 961 AGATCATGGA CAAAGAAGAG ACAAAAGTGT CTGAAGATGA TGAAATGGAG AAGCTGTACA 1021 AATCATTAGA GCAAGCTAGT CTATCTCCTC TTGGGGACCG ACGACCTTCG ACTAAAAAGG 1081 AGTTGAGAAA ATCCTTTGTT AAGCGGTGTA AAAATCCATC TATAAACGAG AAACTCCACA 1141 AAATCCGAAC ATTGAATAGC ACATTAAAGT GTAAAGAACA TGATCTGGCC ATGATTAACC 1201 AGTTGCTGGA TGACCCGAAG CTGACAGCCA GGAAATACAG AGAGTGGAAA GTCATGAACA 1261 CCCTGCTGAT CCAGGACATC TATCAGCAGC AGCGGGCTTC GCCTGCCCCT GATGACACTG 1321 ATGACACCCC CCAGGAACTC AAGAAATCAC CTTCTTCTCC CTCTGTTGAA AATTCCATTT 1381 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1441 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1501 TTTATATATC TTGTGGAAAG GACGATCCTT TGTCAGCGAC ACATACGACA CGCGTTAAGT 1561 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt