Construct: ORF TRCN0000477194
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002849.1_s317c1
- Derived from:
- ccsbBroadEn_10653
- DNA Barcode:
- ACTTCCTGGCCCAATTGGAAAAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LOC613266 (613266)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477194
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 613266 | LOC613266 | uncharacterized LOC613266 | NR_130925.1 | 18.3% | 1_348del;721_2028del | |
| 2 | human | 613266 | LOC613266 | uncharacterized LOC613266 | NR_130924.1 | 16% | 1_645del;1018_2325del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 441
- ORF length:
- 372
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttGGCACCAT GGGAAAGCGA GACCCTAGAG CCCAGCTATT CCTGCCCAGT GCAGGACTCC 121 TCCAGACTTC CAGACTGCAC TGCAGACTGA GGCACTTCCT AGCTGAGCCT CCTTCTTTTC 181 TTCTCTCCTT TCAGCTGCTC CTGCTCCATT GCCCATTTCT CTCACCTTTA TCCTGCACTT 241 CTGCTTCCAT CTTGGCATCT TCTTCCCTCA AGACCCAACC TGACACCATC AGTATTATTA 301 CACTCTTCAT TTCCCAGTGG AGGAGGTGGG GATCCAGGCA AGAAAGACAA AGCGTAAATA 361 TGCTCCCTTT AAAATTTTGT ACTGCTCTAT CGCTTCTGGG TCTTTGGGCT AAGATCAAGT 421 GTAAAAGTTT GTACTACTCT TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 481 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 541 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAACTT CCTGGCCCAA 601 TTGGAAAAGG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt