Construct: ORF TRCN0000477198
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018034.1_s317c1
- Derived from:
- ccsbBroadEn_05632
- DNA Barcode:
- TTTGACCGCTGGCTTCAAATACAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KNOP1 (400506)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477198
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 400506 | KNOP1 | lysine rich nucleolar prote... | NM_001012991.3 | 100% | 100% | |
| 2 | human | 400506 | KNOP1 | lysine rich nucleolar prote... | NM_001348528.2 | 100% | 100% | |
| 3 | human | 400506 | KNOP1 | lysine rich nucleolar prote... | NM_001348529.2 | 100% | 100% | |
| 4 | human | 400506 | KNOP1 | lysine rich nucleolar prote... | NM_001348530.2 | 100% | 100% | |
| 5 | human | 400506 | KNOP1 | lysine rich nucleolar prote... | NM_001348531.2 | 100% | 100% | |
| 6 | human | 400506 | KNOP1 | lysine rich nucleolar prote... | NM_001348532.2 | 94.9% | 94.9% | 916_917ins69 |
| 7 | human | 400506 | KNOP1 | lysine rich nucleolar prote... | NM_001348533.2 | 94.9% | 94.9% | 916_917ins69 |
| 8 | human | 400506 | KNOP1 | lysine rich nucleolar prote... | NM_001348534.2 | 94.9% | 94.9% | 916_917ins69 |
| 9 | human | 400506 | KNOP1 | lysine rich nucleolar prote... | NM_001348535.2 | 94.9% | 94.9% | 916_917ins69 |
| 10 | human | 400506 | KNOP1 | lysine rich nucleolar prote... | NM_001348536.2 | 94.9% | 94.9% | 916_917ins69 |
| 11 | human | 400506 | KNOP1 | lysine rich nucleolar prote... | NM_001348537.2 | 94.9% | 94.9% | 916_917ins69 |
| 12 | human | 400506 | KNOP1 | lysine rich nucleolar prote... | NM_001348527.2 | 88.4% | 88.4% | 1_180del |
| 13 | human | 400506 | KNOP1 | lysine rich nucleolar prote... | XM_024450278.1 | 83.9% | 83.9% | 1_180del;1096_1097ins69 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1443
- ORF length:
- 1374
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gatcaccaag acacacaaag tagaccttgg gctcccagag aagaaaaaga 121 agaagaaagt ggtcaaagaa ccagagactc gatactcagt tttaaacaat gatgattact 181 ttgctgatgt ttctccttta agagctacat ccccctctaa gagtgtggcc catgggcagg 241 cacctgagat gcctctagtg aagaaaaaga agaagaaaaa gaagggtgtc agcacccttt 301 gcgaggagca tgtagaacct gagaccacgc tgcctgctag acggacagag aagtcaccca 361 gcctcaggaa gcaggtgttt ggccacttgg agttcctcag tggggaaaag aaaaataaga 421 agtcacctct agccatgtcc catgcctctg gggtgaaaac ctccccagac cctagacagg 481 gtgaggagga aaccagagtt ggcaagaagc tcaaaaaaca caagaaggaa aaaaaggggg 541 cccaggaccc cacagccttc tcggtccagg acccttggtt ctgtgaggcc agggaggcca 601 gggatgttgg ggacacttgc tcagtgggga agaaggatga ggaacaggca gccttggggc 661 agaaacggaa gcggaagagc cccagagaac acaatgggaa ggtgaagaag aaaaaaaaaa 721 tccaccagga gggagatgcc ctcccaggcc actccaagcc ctccaggtcc atggagagca 781 gccctaggaa aggaagtaaa aagaagccag tcaaagttga ggctccggaa tacatcccca 841 taagtgatga ccctaaggcc tccgcaaaga aaaagatgaa gtccaaaaag aaggtagagc 901 agccagtcat cgaggagcca gctctgaaaa ggaagaaaaa gaagaagagg aaagagagtg 961 gggtagcagg agacccttgg aaggaggaaa cagacacgga cTTAGAGGTG GTGTTGGAAA 1021 AAAAAGGCAA CATGGATGAG GCGCACATAG ACCAGGTGAG GCGAAAGGCC TTGCAAGAAG 1081 AGATCGATCG CGAGTCAGGC AAAACGGAAG CTTCTGAAAC CAGGAAGTGG ACGGGAACCC 1141 AGTTTGGCCA GTGGGATACT GCTGGTTTTG AGAACGAGGA CCAAAAACTG AAATTTCTCA 1201 GACTTATGGG TGGCTTCAAA AACCTGTCCC CTTCGTTCAG CCGCCCCGCC AGCACGATTG 1261 CAAGGCCCAA CATGGCCCTC GGCAAGAAGG CGGCTGACAG CCTGCAGCAG AATCTGCAGC 1321 GGGACTACGA CCGGGCCATG AGCTGGAAGT ACAGCCGGGG AGCCGGCCTC GGCTTCTCCA 1381 CCGCCCCCAA CAAGATCTTT TACATTGACA GGAACGCTTC CAAGTCAGTC AAGCTGGAAG 1441 ATTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1501 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1561 GGCTTTATAT ATCTTGTGGA AAGGACGATT TGACCGCTGG CTTCAAATAC ACACGCGTTA 1621 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt