Construct: ORF TRCN0000477217
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004973.1_s317c1
- Derived from:
- ccsbBroadEn_10660
- DNA Barcode:
- GTGTGAATCAACGAGTAAATCGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LOC646938 (646938)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477217
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 646938 | LOC646938 | TBC1 domain family member 2... | NR_036495.1 | 52.5% | (many diffs) | |
| 2 | human | 23102 | TBC1D2B | TBC1 domain family member 2B | XM_017022022.1 | 10.5% | 8.7% | (many diffs) |
| 3 | human | 23102 | TBC1D2B | TBC1 domain family member 2B | XM_011521387.2 | 8.3% | 6.9% | (many diffs) |
| 4 | human | 23102 | TBC1D2B | TBC1 domain family member 2B | NM_144572.1 | 8% | 6.6% | (many diffs) |
| 5 | human | 23102 | TBC1D2B | TBC1 domain family member 2B | XR_001751162.1 | 4.5% | (many diffs) | |
| 6 | human | 23102 | TBC1D2B | TBC1 domain family member 2B | XR_001751163.1 | 3.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 158
- ORF end:
- 413
- ORF length:
- 255
- Sequence:
-
1 CACTTGATGT AATTCTCCTT GGAATTTGCC CTTTTTGAGT TTGGATCTTG GTTCATTCTC 61 AAGCCTCAGA CAGTGGTTCA AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AGGCTAGCAT 121 CGATTGATCA ACAAGTTTGT ACAAAAAAGT TGGCACCATG GGGACAGGCA GGAAGTCCTT 181 CGGGGCATTT CTGTTTCATC ACAGCTTTTC CTGGGGCTGG AAGCTGATCA GTATCTCCTT 241 TGGGGACCTG AACCCTTTCC CCGTACGCCA GATCCGGAAC CAACGCGCCT ACCACTTGGA 301 GAAAGTCCGG CTGGAGCTGA CCGAGCTGGA GGCCATCCGT GAGGACTTCC TGCATGAGCG 361 GGACACCAGC CCTGACAAGG GTGAGCTGGT CAGTGACGAG GAGGAGGATA CCTTGCCAAC 421 TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA 481 TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT 541 ATCTTGTGGA AAGGACGAGT GTGAATCAAC GAGTAAATCG CCACGCGTTA AGTCgacaat 601 caacctctgg attacaaaat ttgtgaaaga tt