Construct: ORF TRCN0000477239
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012277.1_s317c1
- Derived from:
- ccsbBroadEn_08841
- DNA Barcode:
- CCTTTAATGATATTGGAATTCTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NECAB1 (64168)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477239
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64168 | NECAB1 | N-terminal EF-hand calcium ... | NM_022351.5 | 99.8% | 99.7% | 314A>G;843C>T |
| 2 | human | 64168 | NECAB1 | N-terminal EF-hand calcium ... | XM_011517213.2 | 91.5% | 90% | (many diffs) |
| 3 | mouse | 69352 | Necab1 | N-terminal EF-hand calcium ... | NM_178617.4 | 90.8% | 96% | (many diffs) |
| 4 | mouse | 69352 | Necab1 | N-terminal EF-hand calcium ... | XM_006538234.1 | 85% | 89.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1122
- ORF length:
- 1053
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaagattcc caggagacat cgccgtcctc caacaactcc tcggaggagc 121 tcagctctgc tctgcacctg tccaagggca tgtcgatctt cctcgacata ctgaggagag 181 cagacaaaaa tgatgatgga aaattatcct ttgaagaatt caaagcatat tttgcagatg 241 gtgttctcag tggagaagaa ttacacgagc ttttccatac cattgataca cataatacta 301 ataatcttga cacagaagag ctatgtgaat atttttctca gcacttgggc gagtatgaga 361 atgtactagc agcacttgaa ggcctgaatc tttccatcct gaaggcaatg ggcaaaacaa 421 agaaagacta ccaagaagcc tccaatttgg aacaattcgt aactagattt ttattgaagg 481 aaaccctgaa tcagctgcag tctctccaga attccctgga atgtgccatg gaaactactg 541 aggagcaaac ccgtcaagaa aggcaagggc cagccaagcc agaagtcctg tcgattcaat 601 ggcctggaaa acgatcaagc cgccgagtcc agagacacaa cagcttctcc ccaaacagcc 661 ctcagtttaa tgtcagcggT CCAGGCTTAT TAGAAGAAGA CAACCAGTGG ATGACCCAGA 721 TAAATAGACT CCAGAAATTA ATTGATAGAC TGGAAAAGAA GGATCTCAAA CTCGAACCAC 781 CAGAAGAAGA AATTATTGAA GGGAATACTA AATCTCACAT CATGCTTGTG CAGCGGCAGA 841 TGTCTGTGAT AGAAGAGGAC CTGGAAGAAT TCCAGCTCGC TCTGAAACAC TACGTGGAGA 901 GTGCTTCCTC TCAAAGTGGA TGCTTGCGTA TTTCTATACA GAAGCTTTCA AATGAATCTC 961 GCTACATGAT CTATGAGTTC TGGGAGAATA GTAGTGTATG GAATAGCCAC CTTCAGACAA 1021 ATTATAGCAA GACATTCCAA AGAAGTAATG TGGATTTCTT GGAAACTCCA GAACTCACAT 1081 CTACAATGCT AGTTCCTGCT TCGTGGTGGA TCCTGAACAA CTTGCCAACT TTCTTGTACA 1141 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1201 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1261 AGGACGACCT TTAATGATAT TGGAATTCTG TACGCGTTAA GTCgacaatc aacctctgga 1321 ttacaaaatt tgtgaaagat t