Construct: ORF TRCN0000477239

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012277.1_s317c1
Derived from:
ccsbBroadEn_08841
DNA Barcode:
CCTTTAATGATATTGGAATTCTGT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NECAB1 (64168)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000477239
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 64168 NECAB1 N-terminal EF-hand calcium ... NM_022351.5 99.8% 99.7% 314A>G;843C>T
2 human 64168 NECAB1 N-terminal EF-hand calcium ... XM_011517213.2 91.5% 90% (many diffs)
3 mouse 69352 Necab1 N-terminal EF-hand calcium ... NM_178617.4 90.8% 96% (many diffs)
4 mouse 69352 Necab1 N-terminal EF-hand calcium ... XM_006538234.1 85% 89.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1122
ORF length:
1053
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggaagattcc caggagacat cgccgtcctc caacaactcc tcggaggagc
121tcagctctgc tctgcacctg tccaagggca tgtcgatctt cctcgacata ctgaggagag
181cagacaaaaa tgatgatgga aaattatcct ttgaagaatt caaagcatat tttgcagatg
241gtgttctcag tggagaagaa ttacacgagc ttttccatac cattgataca cataatacta
301ataatcttga cacagaagag ctatgtgaat atttttctca gcacttgggc gagtatgaga
361atgtactagc agcacttgaa ggcctgaatc tttccatcct gaaggcaatg ggcaaaacaa
421agaaagacta ccaagaagcc tccaatttgg aacaattcgt aactagattt ttattgaagg
481aaaccctgaa tcagctgcag tctctccaga attccctgga atgtgccatg gaaactactg
541aggagcaaac ccgtcaagaa aggcaagggc cagccaagcc agaagtcctg tcgattcaat
601ggcctggaaa acgatcaagc cgccgagtcc agagacacaa cagcttctcc ccaaacagcc
661ctcagtttaa tgtcagcggT CCAGGCTTAT TAGAAGAAGA CAACCAGTGG ATGACCCAGA
721TAAATAGACT CCAGAAATTA ATTGATAGAC TGGAAAAGAA GGATCTCAAA CTCGAACCAC
781CAGAAGAAGA AATTATTGAA GGGAATACTA AATCTCACAT CATGCTTGTG CAGCGGCAGA
841TGTCTGTGAT AGAAGAGGAC CTGGAAGAAT TCCAGCTCGC TCTGAAACAC TACGTGGAGA
901GTGCTTCCTC TCAAAGTGGA TGCTTGCGTA TTTCTATACA GAAGCTTTCA AATGAATCTC
961GCTACATGAT CTATGAGTTC TGGGAGAATA GTAGTGTATG GAATAGCCAC CTTCAGACAA
1021ATTATAGCAA GACATTCCAA AGAAGTAATG TGGATTTCTT GGAAACTCCA GAACTCACAT
1081CTACAATGCT AGTTCCTGCT TCGTGGTGGA TCCTGAACAA CTTGCCAACT TTCTTGTACA
1141AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT
1201AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA
1261AGGACGACCT TTAATGATAT TGGAATTCTG TACGCGTTAA GTCgacaatc aacctctgga
1321ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)